BacMap

Definition	Eubacterium eligens ATCC 27750 chromosome, complete genome.
Accession	NC_012778
Length	2,144,190

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The map label for this gene is uxuB [H]

Identifier: 238916607

GI number: 238916607

Start: 664557

End: 665423

Strand: Reverse

Name: uxuB [H]

Synonym: EUBELI_00664

Alternate gene names: 238916607

Gene position: 665423-664557 (Counterclockwise)

Preceding gene: 238916608

Following gene: 238916602

Centisome position: 31.03

GC content: 40.83

Gene sequence:

>867_bases
ATGAAGGTACCATTTAATATTAATCTTTCAGGTAAAACTGCTATTGTAACCGGAGGAAGCGGAACTCTCTGCTCAGCCAT
GGCATATGGACTTGCCGTATGCGGTGCTAAGGTTGCAATAATCGGACGTAACAAAGAAAAGCTTGCTTCTGTTTCTGAGA
AGCTTACTAAAGATGCTCTGGATGAATATAACAAAAATGTTACTGTTAAAGGCTACAGCTGCAATGTAATTAATAAAGAT
GAGCTTTCAGCTGCTTATGAAACTATTAAGAATGAACTCGGCAGCTGTGATATTCTCATTAATGGTGCAGGCGGCAACCA
GCCTGGTGCAATTACTTCCATCGAGATGTTAAAGAAAGAAAAAGAAGATTCTGACTATTCTTTCTGGAATCTTGATGAAG
ACAGAATACGCGATGTTATGGATCTTAATTACATGGGAACTCTTCTTCCTATTCAGGTATTTACTAAGGATATGGTTGAA
AAAAGAAGTGGCAGTATTATCAATATTGCTTCTGTAACTTCTATTCTTCCTCTTACTAAGGTTATGGCTTATGGTAATGC
CAAGAGTGCTATTCTTAATCTTACACAGTGGCTTGCTGTACATTTTGGCGAAAGCGGCATACGCTGCAATGCAATTGCGC
CAGGATTCTACGCTGCCGAGCAGAATCATGATCTCTTATTCAATGCTGATGGCTCATATACAGACCGTGCCAGAAAGATT
ATTGCCGGAACACCTATGGGACGCTTTGGCAATCCTGAAGAGCTTATTGGTGCTGCACTCTTTCTTGCAAGCGATGAAAC
AGCCAGCTTTGTCAATGGTATAGTGTTACCTGTTGACGGCGGATATAGTGCTTATAGCGGAGTGTAG

Upstream 100 bases:

>100_bases
GTATACATGCCTTCTGGCGTAACCCATGGTGTTACATGTCTTGAAGAAGGAATCCTCTGTGATGTATTCAATCCTATGAG
AGAGGATTTTCTTAAGTAAA

Downstream 100 bases:

>100_bases
GATTCTTTAGTTGGAAACTGTATATAATTCAAGGCGATGTCAGTTTTGACATCGCCCTTTTTGTTGCGTTTTTTGTTCAT
TGGACTTTTTGCGTGTTTTT

Product: hypothetical protein

Products: D-Gluconate; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKVPFNINLSGKTAIVTGGSGTLCSAMAYGLAVCGAKVAIIGRNKEKLASVSEKLTKDALDEYNKNVTVKGYSCNVINKD
ELSAAYETIKNELGSCDILINGAGGNQPGAITSIEMLKKEKEDSDYSFWNLDEDRIRDVMDLNYMGTLLPIQVFTKDMVE
KRSGSIINIASVTSILPLTKVMAYGNAKSAILNLTQWLAVHFGESGIRCNAIAPGFYAAEQNHDLLFNADGSYTDRARKI
IAGTPMGRFGNPEELIGAALFLASDETASFVNGIVLPVDGGYSAYSGV

Sequences:

>Translated_288_residues
MKVPFNINLSGKTAIVTGGSGTLCSAMAYGLAVCGAKVAIIGRNKEKLASVSEKLTKDALDEYNKNVTVKGYSCNVINKD
ELSAAYETIKNELGSCDILINGAGGNQPGAITSIEMLKKEKEDSDYSFWNLDEDRIRDVMDLNYMGTLLPIQVFTKDMVE
KRSGSIINIASVTSILPLTKVMAYGNAKSAILNLTQWLAVHFGESGIRCNAIAPGFYAAEQNHDLLFNADGSYTDRARKI
IAGTPMGRFGNPEELIGAALFLASDETASFVNGIVLPVDGGYSAYSGV
>Mature_288_residues
MKVPFNINLSGKTAIVTGGSGTLCSAMAYGLAVCGAKVAIIGRNKEKLASVSEKLTKDALDEYNKNVTVKGYSCNVINKD
ELSAAYETIKNELGSCDILINGAGGNQPGAITSIEMLKKEKEDSDYSFWNLDEDRIRDVMDLNYMGTLLPIQVFTKDMVE
KRSGSIINIASVTSILPLTKVMAYGNAKSAILNLTQWLAVHFGESGIRCNAIAPGFYAAEQNHDLLFNADGSYTDRARKI
IAGTPMGRFGNPEELIGAALFLASDETASFVNGIVLPVDGGYSAYSGV

Specific function: Catalyzes A Reversible Reduction Of 5-Ketoglutanate To Form D-Gluconate. Dependent On Nadp, Almost Inactive With NAD. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=270, Percent_Identity=25.5555555555556, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI19923817, Length=279, Percent_Identity=28.3154121863799, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI66933014, Length=277, Percent_Identity=28.8808664259928, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI15277342, Length=158, Percent_Identity=31.0126582278481, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI59889578, Length=209, Percent_Identity=28.2296650717703, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI10190704, Length=280, Percent_Identity=26.7857142857143, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI32483357, Length=275, Percent_Identity=25.4545454545455, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7705925, Length=278, Percent_Identity=24.8201438848921, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI126723750, Length=273, Percent_Identity=25.2747252747253, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4503301, Length=277, Percent_Identity=26.3537906137184, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1790717, Length=274, Percent_Identity=32.8467153284672, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI1787335, Length=275, Percent_Identity=30.1818181818182, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789208, Length=278, Percent_Identity=32.0143884892086, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87082160, Length=283, Percent_Identity=32.1554770318021, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082100, Length=287, Percent_Identity=26.8292682926829, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787905, Length=277, Percent_Identity=26.7148014440433, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788459, Length=277, Percent_Identity=28.8808664259928, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786812, Length=279, Percent_Identity=25.4480286738351, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI2367175, Length=156, Percent_Identity=33.974358974359, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=282, Percent_Identity=28.7234042553192, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17536025, Length=287, Percent_Identity=27.8745644599303, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=288, Percent_Identity=29.8611111111111, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=277, Percent_Identity=27.7978339350181, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17561272, Length=276, Percent_Identity=28.6231884057971, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17551412, Length=289, Percent_Identity=28.3737024221453, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560220, Length=292, Percent_Identity=26.7123287671233, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17562906, Length=291, Percent_Identity=27.1477663230241, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=292, Percent_Identity=25.6849315068493, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560676, Length=284, Percent_Identity=25, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=290, Percent_Identity=26.8965517241379, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562910, Length=292, Percent_Identity=27.0547945205479, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=290, Percent_Identity=25.5172413793103, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=280, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536651, Length=287, Percent_Identity=26.1324041811847, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17538182, Length=212, Percent_Identity=31.1320754716981, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=291, Percent_Identity=25.7731958762887, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=269, Percent_Identity=27.8810408921933, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17538486, Length=282, Percent_Identity=25.177304964539, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=290, Percent_Identity=26.551724137931, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=287, Percent_Identity=26.4808362369338, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=292, Percent_Identity=24.3150684931507, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=284, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538480, Length=282, Percent_Identity=25.886524822695, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=270, Percent_Identity=27.037037037037, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=283, Percent_Identity=24.3816254416961, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=281, Percent_Identity=25.2669039145907, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324126, Length=273, Percent_Identity=28.9377289377289, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI23397609, Length=275, Percent_Identity=29.0909090909091, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24644337, Length=278, Percent_Identity=28.4172661870504, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571526, Length=275, Percent_Identity=29.4545454545455, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24644339, Length=278, Percent_Identity=28.4172661870504, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357041, Length=284, Percent_Identity=29.5774647887324, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24639444, Length=275, Percent_Identity=28, Blast_Score=88, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355319, Length=277, Percent_Identity=26.7148014440433, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24584744, Length=231, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574402, Length=231, Percent_Identity=23.8095238095238, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161076933, Length=229, Percent_Identity=24.4541484716157, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584748, Length=229, Percent_Identity=24.4541484716157, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.69 [C]

Molecular weight: Translated: 30820; Mature: 30820

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVPFNINLSGKTAIVTGGSGTLCSAMAYGLAVCGAKVAIIGRNKEKLASVSEKLTKDAL
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHH
DEYNKNVTVKGYSCNVINKDELSAAYETIKNELGSCDILINGAGGNQPGAITSIEMLKKE
HHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
KEDSDYSFWNLDEDRIRDVMDLNYMGTLLPIQVFTKDMVEKRSGSIINIASVTSILPLTK
CCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
VMAYGNAKSAILNLTQWLAVHFGESGIRCNAIAPGFYAAEQNHDLLFNADGSYTDRARKI
HHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCEEEECCCCHHHHHHEE
IAGTPMGRFGNPEELIGAALFLASDETASFVNGIVLPVDGGYSAYSGV
EECCCCCCCCCHHHHHHHHHEEECCCHHHHHCCEEEEECCCCHHCCCC
>Mature Secondary Structure
MKVPFNINLSGKTAIVTGGSGTLCSAMAYGLAVCGAKVAIIGRNKEKLASVSEKLTKDAL
CCCCEEEECCCCEEEEECCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHH
DEYNKNVTVKGYSCNVINKDELSAAYETIKNELGSCDILINGAGGNQPGAITSIEMLKKE
HHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHH
KEDSDYSFWNLDEDRIRDVMDLNYMGTLLPIQVFTKDMVEKRSGSIINIASVTSILPLTK
CCCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHH
VMAYGNAKSAILNLTQWLAVHFGESGIRCNAIAPGFYAAEQNHDLLFNADGSYTDRARKI
HHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEECCCCCCEEEECCCCHHHHHHEE
IAGTPMGRFGNPEELIGAALFLASDETASFVNGIVLPVDGGYSAYSGV
EECCCCCCCCCHHHHHHHHHEEECCCHHHHHCCEEEEECCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+; NADH; NADP+; NADPH [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.02 {NADP+}} 20 {D-gluconate}} 0.006 {NADPH}} 0.9 {5-keto-D-gluconate}} [C]

Substrates: 5-Dehydro-D-gluconate; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: 5-Dehydro-D-gluconate + H+ + Nicotinamide adenine dinucleotide phosphate - reduced <==> D-Gluconate + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: Cd2+; Cs2+; Cu2+; Divalent metal ions; Hg2+; Ni2+; p-Chloromercuribenzoate; Sn2+; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579062; 9384377 [H]