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The map label for this gene is moeZ [H]

Identifier: 238916586

GI number: 238916586

Start: 648215

End: 649021

Strand: Direct

Name: moeZ [H]

Synonym: EUBELI_00643

Alternate gene names: 238916586

Gene position: 648215-649021 (Clockwise)

Preceding gene: 238916585

Following gene: 238916587

Centisome position: 30.23

GC content: 41.14

Gene sequence:

>807_bases
ATGGCGTTTACTAATGAACAGCTTGAAAGGTATTCACGCCACATTATTTTAAAGGAAGTTGGAGCTAAAGGCCAGAAGAA
GCTTCTTAATGCGAGCGTGCTTATAATCGGTGCAGGCGGACTTGGTGCTCCTGCAGCTATGTATCTGGCAGCAGCCGGAG
TTGGAACAATCGGTATTGTTGATGCTGATGAGGTTGATTTATCTAATCTTCAGAGACAGATTATCCATTCAACAGCAGAT
ATCGGTAAGGCTAAGGTTAAATCTGCTAAAGAAACAATGGAAGCAATCAATCCTGATGTAACAGTTAAGACTTACAGAGA
ATTCGTTACATCTGAGAATGTTATGGAGCTTATCAAGGATTATGATTTCATCATTGATGGAACAGATAATTTCCCTGCAA
AATTCCTTATTAACGATGCGTGTGTTATGGCTGGAAAGCCTTTCTCTCATGCCGGAATTATAAGATTCAAGGGTCAGCTT
ATGACTTATGTACCAGGAGAAGGACCATGCTACAGATGTGTATTCAAGAACCCACCACCAAAGGACGCAGTTCCTACATG
TAAGCAGGCAGGCGTTATCGGAGCAATGGGTGGTGTTATCGGAAGCCTTCAGGCAATGGAAGCTATCAAGTACATCATTG
GCAAGGGAGACCTTCTTACAGGAAGACTTCTTACATATGATGCTTTAAAGATGGAATTCCATACAATTAAGCTTCCAAAG
GACCATCATTGTGCTATATGCGGAGATAATCCTACAATTCATGAGCTTATTGATTATGAACAGGCTGAATGTGATATGCA
TATGTAG

Upstream 100 bases:

>100_bases
TTAATGATGATTTTGTTGAGAGAGATGATTTTGAGACAACAAAGTTAAATGAAGGCGATGCAGTTGAGTTCTTATATTTC
ATGGGAGGAGGAGCATACTA

Downstream 100 bases:

>100_bases
GAGAAAGATATATCAAGGCAGAATAATGGGATAATCATGTGCAGGCACGCTTTCGCTACTTGTCGTTCACAGCGGTACTA
AGCTCGCCAACGCCGAAGGC

Product: thiamine biosynthesis protein ThiF

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MAFTNEQLERYSRHIILKEVGAKGQKKLLNASVLIIGAGGLGAPAAMYLAAAGVGTIGIVDADEVDLSNLQRQIIHSTAD
IGKAKVKSAKETMEAINPDVTVKTYREFVTSENVMELIKDYDFIIDGTDNFPAKFLINDACVMAGKPFSHAGIIRFKGQL
MTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGKGDLLTGRLLTYDALKMEFHTIKLPK
DHHCAICGDNPTIHELIDYEQAECDMHM

Sequences:

>Translated_268_residues
MAFTNEQLERYSRHIILKEVGAKGQKKLLNASVLIIGAGGLGAPAAMYLAAAGVGTIGIVDADEVDLSNLQRQIIHSTAD
IGKAKVKSAKETMEAINPDVTVKTYREFVTSENVMELIKDYDFIIDGTDNFPAKFLINDACVMAGKPFSHAGIIRFKGQL
MTYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGKGDLLTGRLLTYDALKMEFHTIKLPK
DHHCAICGDNPTIHELIDYEQAECDMHM
>Mature_267_residues
AFTNEQLERYSRHIILKEVGAKGQKKLLNASVLIIGAGGLGAPAAMYLAAAGVGTIGIVDADEVDLSNLQRQIIHSTADI
GKAKVKSAKETMEAINPDVTVKTYREFVTSENVMELIKDYDFIIDGTDNFPAKFLINDACVMAGKPFSHAGIIRFKGQLM
TYVPGEGPCYRCVFKNPPPKDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGKGDLLTGRLLTYDALKMEFHTIKLPKD
HHCAICGDNPTIHELIDYEQAECDMHM

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=260, Percent_Identity=44.2307692307692, Blast_Score=229, Evalue=3e-60,
Organism=Homo sapiens, GI13376212, Length=257, Percent_Identity=28.7937743190661, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4885649, Length=156, Percent_Identity=28.8461538461538, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI38327032, Length=251, Percent_Identity=28.2868525896414, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI38045942, Length=172, Percent_Identity=29.0697674418605, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI38045944, Length=172, Percent_Identity=29.0697674418605, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI23510338, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI23510340, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI150417996, Length=206, Percent_Identity=26.6990291262136, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787048, Length=248, Percent_Identity=40.3225806451613, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI87082356, Length=240, Percent_Identity=40.8333333333333, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789177, Length=133, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=252, Percent_Identity=44.047619047619, Blast_Score=229, Evalue=9e-61,
Organism=Caenorhabditis elegans, GI193203301, Length=164, Percent_Identity=27.4390243902439, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71984476, Length=185, Percent_Identity=31.3513513513514, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI86565433, Length=176, Percent_Identity=26.7045454545455, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI86565431, Length=176, Percent_Identity=26.7045454545455, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508819, Length=236, Percent_Identity=23.728813559322, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=254, Percent_Identity=39.3700787401575, Blast_Score=187, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322639, Length=189, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=29.4117647058824, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325323, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24582879, Length=266, Percent_Identity=46.9924812030075, Blast_Score=260, Evalue=9e-70,
Organism=Drosophila melanogaster, GI24660640, Length=167, Percent_Identity=32.3353293413174, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28573937, Length=186, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20129973, Length=174, Percent_Identity=29.3103448275862, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24641311, Length=257, Percent_Identity=27.2373540856031, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 29105; Mature: 28974

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFTNEQLERYSRHIILKEVGAKGQKKLLNASVLIIGAGGLGAPAAMYLAAAGVGTIGIV
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCEEEEE
DADEVDLSNLQRQIIHSTADIGKAKVKSAKETMEAINPDVTVKTYREFVTSENVMELIKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHC
YDFIIDGTDNFPAKFLINDACVMAGKPFSHAGIIRFKGQLMTYVPGEGPCYRCVFKNPPP
CCEEEECCCCCCCEEEECCHHEECCCCCCCCCEEEEECEEEEEECCCCCEEEEEECCCCC
KDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGKGDLLTGRLLTYDALKMEFHTIKLPK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHEEEEEEEECCC
DHHCAICGDNPTIHELIDYEQAECDMHM
CCEEEEECCCCHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
AFTNEQLERYSRHIILKEVGAKGQKKLLNASVLIIGAGGLGAPAAMYLAAAGVGTIGIV
CCCHHHHHHHHHHHHHHHHCCCHHHHHHCCEEEEEECCCCCCHHHHHHHHCCCCEEEEE
DADEVDLSNLQRQIIHSTADIGKAKVKSAKETMEAINPDVTVKTYREFVTSENVMELIKD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHC
YDFIIDGTDNFPAKFLINDACVMAGKPFSHAGIIRFKGQLMTYVPGEGPCYRCVFKNPPP
CCEEEECCCCCCCEEEECCHHEECCCCCCCCCEEEEECEEEEEECCCCCEEEEEECCCCC
KDAVPTCKQAGVIGAMGGVIGSLQAMEAIKYIIGKGDLLTGRLLTYDALKMEFHTIKLPK
CCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHEEEEEEEECCC
DHHCAICGDNPTIHELIDYEQAECDMHM
CCEEEEECCCCHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]