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The map label for this gene is nirB [C]

Identifier: 238916582

GI number: 238916582

Start: 646487

End: 647386

Strand: Direct

Name: nirB [C]

Synonym: EUBELI_00639

Alternate gene names: 238916582

Gene position: 646487-647386 (Clockwise)

Preceding gene: 238916581

Following gene: 238916583

Centisome position: 30.15

GC content: 42.22

Gene sequence:

>900_bases
ATGAATAGTGCATGGCACGGAAATGAGGATATTATGGCAGAGAAAGTTGATTATGGAACACTTAAGAAGGGCGGATTCAT
GAGACAGAAGCAGAAGAATAACTTCTCACTTCGTCTTGCAGTTGTAGGAGGATATCTTACAGCCGAGAATCTTACCAAGA
TTGCTGAAGTTGCAGAAAAATATGGAGACGGACATGTCCATCTTACATCAAGACAGGGCGTTGAGATTCCATTTATTAAA
CTTAAAGATATAGATGCTGTTAAGGAAGAACTTGCAGAGGGCGGCTGCAGACCAGGTGTGTGTGGTCCGAGAGTGCGTAC
AGTCACAGCCTGTCAGGGCAATACAATATGTCCTAGCGGTAACATAGATTCATATGACATTGCAGTTAAGTTAGACGAAA
GATACTTCGGAAGAGAACTTCCACATAAGTTCAAATTCGGTGTTACAGGATGTCAGAACAACTGTCTTAAGGCAGAGGAG
AATGATGTTGGTATCAAGGGTGCTGCTGATGTTAAGTGGATTGAAGACAAATGTATTGGCTGTGGTGTATGTGAGAAGGC
CTGTCGTACAGGTGCAATCACAATGCAGGATGGCAAGGTTGCAGTTGATTATGACAAATGTAATTACTGCGGCAGATGTG
CTAAATCTTGTCCAACAGATGCGTGGGACGCACCTTCTGCATATATCATTTCATTTGCAGGTACATTTGGTAATTCTATA
AGCAAGGGTGAATCACCACTTCCGCTTATCAGAAATGAAGAACAGTTATTCCGTGTCTGTGATGCAGCAATCCAGTTCTT
TGCAGACAACGCAAAGCCAAGTGAAAGATTTAAGTTCACACTCGACAGAGTTGGAAGAGAAGAATTCTATAAGAAGATTA
AGGAGGCATACAATGGCTGA

Upstream 100 bases:

>100_bases
GCTTCAGGAGTGTATGACGATCTGATAAGAGTCAGCGTAGGAATAGAGGATATAGATGATCTTAAGGAAGATTTCAGTGC
AGCCTTAGAGTCTACTGATA

Downstream 100 bases:

>100_bases
TTTTGAGATTGATGATACAGTAGATATTACAGATGTGGTCTGCCCAACAACATTTGTTAAGGCAAAGGTAGCAATTGAGG
AGTTAGATGACGGACAGATT

Product: coenzyme F420 hydrogenase

Products: reduced coenzyme F420

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNSAWHGNEDIMAEKVDYGTLKKGGFMRQKQKNNFSLRLAVVGGYLTAENLTKIAEVAEKYGDGHVHLTSRQGVEIPFIK
LKDIDAVKEELAEGGCRPGVCGPRVRTVTACQGNTICPSGNIDSYDIAVKLDERYFGRELPHKFKFGVTGCQNNCLKAEE
NDVGIKGAADVKWIEDKCIGCGVCEKACRTGAITMQDGKVAVDYDKCNYCGRCAKSCPTDAWDAPSAYIISFAGTFGNSI
SKGESPLPLIRNEEQLFRVCDAAIQFFADNAKPSERFKFTLDRVGREEFYKKIKEAYNG

Sequences:

>Translated_299_residues
MNSAWHGNEDIMAEKVDYGTLKKGGFMRQKQKNNFSLRLAVVGGYLTAENLTKIAEVAEKYGDGHVHLTSRQGVEIPFIK
LKDIDAVKEELAEGGCRPGVCGPRVRTVTACQGNTICPSGNIDSYDIAVKLDERYFGRELPHKFKFGVTGCQNNCLKAEE
NDVGIKGAADVKWIEDKCIGCGVCEKACRTGAITMQDGKVAVDYDKCNYCGRCAKSCPTDAWDAPSAYIISFAGTFGNSI
SKGESPLPLIRNEEQLFRVCDAAIQFFADNAKPSERFKFTLDRVGREEFYKKIKEAYNG
>Mature_299_residues
MNSAWHGNEDIMAEKVDYGTLKKGGFMRQKQKNNFSLRLAVVGGYLTAENLTKIAEVAEKYGDGHVHLTSRQGVEIPFIK
LKDIDAVKEELAEGGCRPGVCGPRVRTVTACQGNTICPSGNIDSYDIAVKLDERYFGRELPHKFKFGVTGCQNNCLKAEE
NDVGIKGAADVKWIEDKCIGCGVCEKACRTGAITMQDGKVAVDYDKCNYCGRCAKSCPTDAWDAPSAYIISFAGTFGNSI
SKGESPLPLIRNEEQLFRVCDAAIQFFADNAKPSERFKFTLDRVGREEFYKKIKEAYNG

Specific function: Nitrate assimilation (denitrification); second step. [C]

COG id: COG2221

COG function: function code C; Dissimilatory sulfite reductase (desulfoviridin), alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1789765, Length=142, Percent_Identity=31.6901408450704, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR007516
- InterPro:   IPR007525
- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF00037 Fer4; PF04432 FrhB_FdhB_C; PF04422 FrhB_FdhB_N; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: 1.12.98.1

Molecular weight: Translated: 32965; Mature: 32965

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00365 NIR_SIR ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAWHGNEDIMAEKVDYGTLKKGGFMRQKQKNNFSLRLAVVGGYLTAENLTKIAEVAEK
CCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHH
YGDGHVHLTSRQGVEIPFIKLKDIDAVKEELAEGGCRPGVCGPRVRTVTACQGNTICPSG
HCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEECCCC
NIDSYDIAVKLDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGAADVKWIEDKCIG
CCCCEEEEEEECCHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCCC
CGVCEKACRTGAITMQDGKVAVDYDKCNYCGRCAKSCPTDAWDAPSAYIISFAGTFGNSI
CHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEECHHCCCC
SKGESPLPLIRNEEQLFRVCDAAIQFFADNAKPSERFKFTLDRVGREEFYKKIKEAYNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSAWHGNEDIMAEKVDYGTLKKGGFMRQKQKNNFSLRLAVVGGYLTAENLTKIAEVAEK
CCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHH
YGDGHVHLTSRQGVEIPFIKLKDIDAVKEELAEGGCRPGVCGPRVRTVTACQGNTICPSG
HCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEECCCC
NIDSYDIAVKLDERYFGRELPHKFKFGVTGCQNNCLKAEENDVGIKGAADVKWIEDKCIG
CCCCEEEEEEECCHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCCC
CGVCEKACRTGAITMQDGKVAVDYDKCNYCGRCAKSCPTDAWDAPSAYIISFAGTFGNSI
CHHHHHHHHCCCEEEECCEEEEEECCCCHHHHHHHCCCCCCCCCCCEEEEEEECHHCCCC
SKGESPLPLIRNEEQLFRVCDAAIQFFADNAKPSERFKFTLDRVGREEFYKKIKEAYNG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2; coenzyme F420

Specific reaction: H2 + coenzyme F420 = reduced coenzyme F420

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]