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The map label for this gene is rlmI [H]

Identifier: 238916542

GI number: 238916542

Start: 611746

End: 613008

Strand: Direct

Name: rlmI [H]

Synonym: EUBELI_00599

Alternate gene names: 238916542

Gene position: 611746-613008 (Clockwise)

Preceding gene: 238916541

Following gene: 238916546

Centisome position: 28.53

GC content: 41.33

Gene sequence:

>1263_bases
ATGAATAATTATGATAAATGTCAGAAAAATCTGGCAGGAAGGAACAGAATGGAAGATTTATTCAGCGTATGCGGAAAGAT
ACGATTAAGAAAAGGTGAAGGAAGAACCTTAAAGGCAGGCGGTGCCTGGGTATATGATAACGAAATTGAATGGATGTCTG
ACGGAATAATTGACGGACATCTTGTTGAGGTGCTCGATTTTGATGATTATTTCATGGGAATCGGATTTATTAACAGAAAA
TCCAAGATTACAGTAAGAATGCTTGCAAGACACACTGATGTTGTTGATGAGGCATTTATTGAGGAGAGAGTAAGGGCTGC
GGTTGAGTACAGATTTGACACAGTAGATACCTCTGCCTGCCGTCTTATATTTGGAGAGGCAGATTTTCTGCCGGGGCTTG
TTATAGATAAGTATTCAGATGTGCTTGTTGTAGAATCGCTTGCTTTAGGAATTGACAGATTTAAGCCACTTATTGTCGAA
AAGGTGAAGGAAATCCTTCATGAAAAAGGCATTGATATCCGTGGTGTATATGAAAGAAGCGATGCCAAAGTCCGTACTCT
TGAAGGGATGGAGAGAGTAAAGGGCTTTATTGGTGATGAATTTGACACTAATGTTGAGATAGTTGAGAATGGTGTCCACT
ACATGATAGATGTTGTGAATGGACAGAAAACCGGGTTCTTTCTTGATCAGAAGTACAACAGACTTGCAATGCAGAAGCTG
TGCAAGGACAAGAGGGTATTAGACTGTTTCACCCATATGGGTACATTTGCATTAAATGCAGGAATTGCAGGTGCTAAAGA
AGTTACTGGACTTGATATATCAGAATATGCTGTTGAACAGGCGAGAGCCAATGCCAAGCTTAACGGACTTGAGGATACAG
TACATTTTAAATGTGCAAATGTATTGGACGAGCTGCCAAAGCTTAACGAAGCAGGTGAGAAATATGATGTTGTAATACTT
GACCCGCCGGCATTTACCAAATCGAGGGAAGCTACAAAGAACGCAATCAAGGGCTACAGGGAAATCAATATGAAGGGACT
TAAACTTGTCAAAGACGGCGGCTATCTTGCAAGCTGTTCATGCTCACACTTCATGACCCAGGAGCTTCTTACCAAGACTA
TTAAGGAAGCCGCTAAAGCTGTACATAAAAGACTCCGTCAGGTCGAGTTCAGGACACAGTCACCTGACCACCCAATACTG
TGGGAGGCGCAGGAGAGCTATTATCTGAAATTCTTTATCTTTCAGGTTGTTGATGAAAAATGA

Upstream 100 bases:

>100_bases
GACTACATTTTCATGGATTAAGCTCGTGTCGCTTCTTATTACGGTTGCAGGAGTATGTATGTTTTATTTTCTTGGTAATC
CGTCAACGGATTAATATATT

Downstream 100 bases:

>100_bases
TACAGGCATTAATTTAAACTATCCAGGACCGTTAATCTGAGTCTTTTATAATCTTTTATGATGTTTTGTTATAATTAATT
ATATGTATCCGCAAACTATT

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNNYDKCQKNLAGRNRMEDLFSVCGKIRLRKGEGRTLKAGGAWVYDNEIEWMSDGIIDGHLVEVLDFDDYFMGIGFINRK
SKITVRMLARHTDVVDEAFIEERVRAAVEYRFDTVDTSACRLIFGEADFLPGLVIDKYSDVLVVESLALGIDRFKPLIVE
KVKEILHEKGIDIRGVYERSDAKVRTLEGMERVKGFIGDEFDTNVEIVENGVHYMIDVVNGQKTGFFLDQKYNRLAMQKL
CKDKRVLDCFTHMGTFALNAGIAGAKEVTGLDISEYAVEQARANAKLNGLEDTVHFKCANVLDELPKLNEAGEKYDVVIL
DPPAFTKSREATKNAIKGYREINMKGLKLVKDGGYLASCSCSHFMTQELLTKTIKEAAKAVHKRLRQVEFRTQSPDHPIL
WEAQESYYLKFFIFQVVDEK

Sequences:

>Translated_420_residues
MNNYDKCQKNLAGRNRMEDLFSVCGKIRLRKGEGRTLKAGGAWVYDNEIEWMSDGIIDGHLVEVLDFDDYFMGIGFINRK
SKITVRMLARHTDVVDEAFIEERVRAAVEYRFDTVDTSACRLIFGEADFLPGLVIDKYSDVLVVESLALGIDRFKPLIVE
KVKEILHEKGIDIRGVYERSDAKVRTLEGMERVKGFIGDEFDTNVEIVENGVHYMIDVVNGQKTGFFLDQKYNRLAMQKL
CKDKRVLDCFTHMGTFALNAGIAGAKEVTGLDISEYAVEQARANAKLNGLEDTVHFKCANVLDELPKLNEAGEKYDVVIL
DPPAFTKSREATKNAIKGYREINMKGLKLVKDGGYLASCSCSHFMTQELLTKTIKEAAKAVHKRLRQVEFRTQSPDHPIL
WEAQESYYLKFFIFQVVDEK
>Mature_420_residues
MNNYDKCQKNLAGRNRMEDLFSVCGKIRLRKGEGRTLKAGGAWVYDNEIEWMSDGIIDGHLVEVLDFDDYFMGIGFINRK
SKITVRMLARHTDVVDEAFIEERVRAAVEYRFDTVDTSACRLIFGEADFLPGLVIDKYSDVLVVESLALGIDRFKPLIVE
KVKEILHEKGIDIRGVYERSDAKVRTLEGMERVKGFIGDEFDTNVEIVENGVHYMIDVVNGQKTGFFLDQKYNRLAMQKL
CKDKRVLDCFTHMGTFALNAGIAGAKEVTGLDISEYAVEQARANAKLNGLEDTVHFKCANVLDELPKLNEAGEKYDVVIL
DPPAFTKSREATKNAIKGYREINMKGLKLVKDGGYLASCSCSHFMTQELLTKTIKEAAKAVHKRLRQVEFRTQSPDHPIL
WEAQESYYLKFFIFQVVDEK

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=400, Percent_Identity=32, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1787181, Length=207, Percent_Identity=28.9855072463768, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 47748; Mature: 47748

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYDKCQKNLAGRNRMEDLFSVCGKIRLRKGEGRTLKAGGAWVYDNEIEWMSDGIIDGH
CCCHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCEEECCCEEEECCCCHHHHCCCCCCC
LVEVLDFDDYFMGIGFINRKSKITVRMLARHTDVVDEAFIEERVRAAVEYRFDTVDTSAC
EEEEECCCCHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RLIFGEADFLPGLVIDKYSDVLVVESLALGIDRFKPLIVEKVKEILHEKGIDIRGVYERS
EEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DAKVRTLEGMERVKGFIGDEFDTNVEIVENGVHYMIDVVNGQKTGFFLDQKYNRLAMQKL
CCHHHHHHHHHHHHHHHCCCCCCCHHEECCCCEEEEEEECCCCCCEEHHHHHHHHHHHHH
CKDKRVLDCFTHMGTFALNAGIAGAKEVTGLDISEYAVEQARANAKLNGLEDTVHFKCAN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VLDELPKLNEAGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKLVKDGGYLASCS
HHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECC
CSHFMTQELLTKTIKEAAKAVHKRLRQVEFRTQSPDHPILWEAQESYYLKFFIFQVVDEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHEEEHHHCCC
>Mature Secondary Structure
MNNYDKCQKNLAGRNRMEDLFSVCGKIRLRKGEGRTLKAGGAWVYDNEIEWMSDGIIDGH
CCCHHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCEEECCCEEEECCCCHHHHCCCCCCC
LVEVLDFDDYFMGIGFINRKSKITVRMLARHTDVVDEAFIEERVRAAVEYRFDTVDTSAC
EEEEECCCCHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
RLIFGEADFLPGLVIDKYSDVLVVESLALGIDRFKPLIVEKVKEILHEKGIDIRGVYERS
EEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECC
DAKVRTLEGMERVKGFIGDEFDTNVEIVENGVHYMIDVVNGQKTGFFLDQKYNRLAMQKL
CCHHHHHHHHHHHHHHHCCCCCCCHHEECCCCEEEEEEECCCCCCEEHHHHHHHHHHHHH
CKDKRVLDCFTHMGTFALNAGIAGAKEVTGLDISEYAVEQARANAKLNGLEDTVHFKCAN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHH
VLDELPKLNEAGEKYDVVILDPPAFTKSREATKNAIKGYREINMKGLKLVKDGGYLASCS
HHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECC
CSHFMTQELLTKTIKEAAKAVHKRLRQVEFRTQSPDHPILWEAQESYYLKFFIFQVVDEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA