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The map label for this gene is prfC [H]

Identifier: 238916500

GI number: 238916500

Start: 566151

End: 567743

Strand: Direct

Name: prfC [H]

Synonym: EUBELI_00557

Alternate gene names: 238916500

Gene position: 566151-567743 (Clockwise)

Preceding gene: 238916499

Following gene: 238916501

Centisome position: 26.4

GC content: 39.99

Gene sequence:

>1593_bases
GTGAGTTTAGAAAGTGAAATTAAGAAAAGAAGAACTTTTGCCATTATCTCTCACCCGGATGCTGGTAAGACAACATTAAC
AGAGAAGTTCCTGCTTTATGGAGGAGCCATTAACCTTGCAGGTTCTGTAAAGGGTAAGAAGACTGCCAAGCATGCAGTTT
CCGACTGGATGGAGATTGAGAAGGAAAGAGGTATTTCGGTAACTTCTTCTGTTCTTCAGTTTAACTATGAAGGTTACTGT
ATCAATATTCTTGATACTCCGGGACATGAGGATTTCTCAGAGGATACCTACAGAACTCTTATGGCAGCAGATTCAGCAGT
CATGGTTATTGATGCTTCTAAGGGTGTTGAGAAACAGACTATCAAGCTTTTCAAGGTATGTGTTATGAGACACATTCCTA
TATTTACATTTATCAATAAGATGGATAGAGAAGCTAATGATCCATTTGAGCTTCTTGATGAAATTGAGAGTGTACTTGGA
ATCTCTACATGCCCAATTAACTGGCCTATCGGATGCGGTAAGGAATTCAAGGGTGTGTATGACCGTAAAGAGAAAAATGT
TTCTTTATTCAAGGCAGCCATGAATGGTCAGAAGGAAGTTGATACTGAGATTGTTGATGCGTCAGACGAGGCAGTTTTTA
AGGACAGAATTGGTGATGCTTTCTATGACAAGCTTCAGGAAGATATTGAGCTGTTAGACGGAGCAAGTGCGGAGTTTGAT
CAGGAGATGGTAAGTGCAGGTGACCTTACACCTGTATTCTTTGGTTCAGCGCTTACTAACTTTGGTGTACAGACTTTCCT
TGAGCATTTCTTATCAATGACAACTTCACCACTTCCAAGAAAGTCTGATCAGGGAATTATTGATCCATTTACTGAGGATT
TCTCAGCATTTGTATTCAAGATTCAGGCTAACATGAATAAAGCACATAGAGACAGAATTGCTTTCATGAGAATATGTTCC
GGTAAGTTTGAAGCCGGAATGGAAGCCAACCATGTTCAGGGTGGCAAGAAAATAAGACTCAGCCAGCCACAGCAGATGAT
GGCGGAGGAGAGACATATTGTAGATGAGGCATATGCAGGAGATATTATAGGTGTATTTGATCCGGGTATTTTCTCTATTG
GTGATACTATATGCAAGTCTAATAAGAAATTTGCATTTGAGGGTATACCTACATTTGCACCAGAACATTTTGCAAGAGTA
AGACAGCTCGATACTATGAAGAGAAAGCAGTTTATCAAGGGTATTAACCAGATTGCACAGGAAGGTGCAATTCAGATATT
CCAGGAGTTCAATACAGGAATGGAAGAGATTATCGTGGGAGTTGTCGGTGTTCTTCAGTTTGAGGTTCTTGAATACCGTC
TTAAGAATGAATACGGTGTTGATATCAGACTTGAGCCGCTTCCATATGAGCATATCCGCTGGATTGAGAATCCTGAGGAA
ATTGATGTGAACAGAATTGTCGGTACATCAGATATGAAGAAGATTAAAGACCTTAAGGGCAATCCACTTCTTGTATTTGC
CAATAGCTGGTCTGTTAATATGGTACTTGAGAGAAATGAGGGCTTAAAGCTTTCAGAATTTGGAAGAAACTAG

Upstream 100 bases:

>100_bases
TGAAGCTAAGACTGCGTCACAGGCTTATATTATGTATATGCCGGGAAAGGCATGAATTATTTGATAAGCTATGTATTATG
TGATATAAAGGAGTAAAACA

Downstream 100 bases:

>100_bases
TTGTATGATACATTTAGGAGTCAGGTGATTACATGAAATCAGTATACAAATGTAGTGTAAAAAGAATAGCAGCACTGGCA
GTGATGGTATGTATGCTTGT

Product: peptide chain release factor RF-3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSLESEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSVKGKKTAKHAVSDWMEIEKERGISVTSSVLQFNYEGYC
INILDTPGHEDFSEDTYRTLMAADSAVMVIDASKGVEKQTIKLFKVCVMRHIPIFTFINKMDREANDPFELLDEIESVLG
ISTCPINWPIGCGKEFKGVYDRKEKNVSLFKAAMNGQKEVDTEIVDASDEAVFKDRIGDAFYDKLQEDIELLDGASAEFD
QEMVSAGDLTPVFFGSALTNFGVQTFLEHFLSMTTSPLPRKSDQGIIDPFTEDFSAFVFKIQANMNKAHRDRIAFMRICS
GKFEAGMEANHVQGGKKIRLSQPQQMMAEERHIVDEAYAGDIIGVFDPGIFSIGDTICKSNKKFAFEGIPTFAPEHFARV
RQLDTMKRKQFIKGINQIAQEGAIQIFQEFNTGMEEIIVGVVGVLQFEVLEYRLKNEYGVDIRLEPLPYEHIRWIENPEE
IDVNRIVGTSDMKKIKDLKGNPLLVFANSWSVNMVLERNEGLKLSEFGRN

Sequences:

>Translated_530_residues
MSLESEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSVKGKKTAKHAVSDWMEIEKERGISVTSSVLQFNYEGYC
INILDTPGHEDFSEDTYRTLMAADSAVMVIDASKGVEKQTIKLFKVCVMRHIPIFTFINKMDREANDPFELLDEIESVLG
ISTCPINWPIGCGKEFKGVYDRKEKNVSLFKAAMNGQKEVDTEIVDASDEAVFKDRIGDAFYDKLQEDIELLDGASAEFD
QEMVSAGDLTPVFFGSALTNFGVQTFLEHFLSMTTSPLPRKSDQGIIDPFTEDFSAFVFKIQANMNKAHRDRIAFMRICS
GKFEAGMEANHVQGGKKIRLSQPQQMMAEERHIVDEAYAGDIIGVFDPGIFSIGDTICKSNKKFAFEGIPTFAPEHFARV
RQLDTMKRKQFIKGINQIAQEGAIQIFQEFNTGMEEIIVGVVGVLQFEVLEYRLKNEYGVDIRLEPLPYEHIRWIENPEE
IDVNRIVGTSDMKKIKDLKGNPLLVFANSWSVNMVLERNEGLKLSEFGRN
>Mature_529_residues
SLESEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSVKGKKTAKHAVSDWMEIEKERGISVTSSVLQFNYEGYCI
NILDTPGHEDFSEDTYRTLMAADSAVMVIDASKGVEKQTIKLFKVCVMRHIPIFTFINKMDREANDPFELLDEIESVLGI
STCPINWPIGCGKEFKGVYDRKEKNVSLFKAAMNGQKEVDTEIVDASDEAVFKDRIGDAFYDKLQEDIELLDGASAEFDQ
EMVSAGDLTPVFFGSALTNFGVQTFLEHFLSMTTSPLPRKSDQGIIDPFTEDFSAFVFKIQANMNKAHRDRIAFMRICSG
KFEAGMEANHVQGGKKIRLSQPQQMMAEERHIVDEAYAGDIIGVFDPGIFSIGDTICKSNKKFAFEGIPTFAPEHFARVR
QLDTMKRKQFIKGINQIAQEGAIQIFQEFNTGMEEIIVGVVGVLQFEVLEYRLKNEYGVDIRLEPLPYEHIRWIENPEEI
DVNRIVGTSDMKKIKDLKGNPLLVFANSWSVNMVLERNEGLKLSEFGRN

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=484, Percent_Identity=29.9586776859504, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI19923640, Length=505, Percent_Identity=27.3267326732673, Blast_Score=165, Evalue=9e-41,
Organism=Homo sapiens, GI25306283, Length=390, Percent_Identity=30.5128205128205, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI25306287, Length=290, Percent_Identity=33.1034482758621, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=35.6687898089172, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI94966754, Length=149, Percent_Identity=32.2147651006711, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=30.2013422818792, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI310132016, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI310110807, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI310123363, Length=125, Percent_Identity=33.6, Blast_Score=70, Evalue=7e-12,
Organism=Escherichia coli, GI1790835, Length=471, Percent_Identity=50.1061571125265, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1789738, Length=492, Percent_Identity=26.8292682926829, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=34.375, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI48994988, Length=394, Percent_Identity=24.1116751269036, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=488, Percent_Identity=28.0737704918033, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI17556745, Length=495, Percent_Identity=25.0505050505051, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=139, Percent_Identity=34.5323741007194, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.8136645962733, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=30.0751879699248, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=491, Percent_Identity=26.4765784114053, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=528, Percent_Identity=27.0833333333333, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6323320, Length=166, Percent_Identity=28.9156626506024, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=29.746835443038, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=29.746835443038, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582462, Length=493, Percent_Identity=28.1947261663286, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI221458488, Length=530, Percent_Identity=24.1509433962264, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=33.75, Blast_Score=106, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24585709, Length=158, Percent_Identity=31.0126582278481, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585711, Length=158, Percent_Identity=31.0126582278481, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585713, Length=158, Percent_Identity=31.0126582278481, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59728; Mature: 59597

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLESEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSVKGKKTAKHAVSDWMEIE
CCCHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHH
KERGISVTSSVLQFNYEGYCINILDTPGHEDFSEDTYRTLMAADSAVMVIDASKGVEKQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHH
IKLFKVCVMRHIPIFTFINKMDREANDPFELLDEIESVLGISTCPINWPIGCGKEFKGVY
HHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
DRKEKNVSLFKAAMNGQKEVDTEIVDASDEAVFKDRIGDAFYDKLQEDIELLDGASAEFD
HHCCCCHHHHHHHHCCCHHCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHH
QEMVSAGDLTPVFFGSALTNFGVQTFLEHFLSMTTSPLPRKSDQGIIDPFTEDFSAFVFK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEE
IQANMNKAHRDRIAFMRICSGKFEAGMEANHVQGGKKIRLSQPQQMMAEERHIVDEAYAG
EECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFSIGDTICKSNKKFAFEGIPTFAPEHFARVRQLDTMKRKQFIKGINQIAQ
CEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAIQIFQEFNTGMEEIIVGVVGVLQFEVLEYRLKNEYGVDIRLEPLPYEHIRWIENPEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCC
IDVNRIVGTSDMKKIKDLKGNPLLVFANSWSVNMVLERNEGLKLSEFGRN
CCHHHEECCHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHCCCCC
>Mature Secondary Structure 
SLESEIKKRRTFAIISHPDAGKTTLTEKFLLYGGAINLAGSVKGKKTAKHAVSDWMEIE
CCHHHHHHCCEEEEEECCCCCCHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHH
KERGISVTSSVLQFNYEGYCINILDTPGHEDFSEDTYRTLMAADSAVMVIDASKGVEKQT
HHCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHH
IKLFKVCVMRHIPIFTFINKMDREANDPFELLDEIESVLGISTCPINWPIGCGKEFKGVY
HHHHHHHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHH
DRKEKNVSLFKAAMNGQKEVDTEIVDASDEAVFKDRIGDAFYDKLQEDIELLDGASAEFD
HHCCCCHHHHHHHHCCCHHCCHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHH
QEMVSAGDLTPVFFGSALTNFGVQTFLEHFLSMTTSPLPRKSDQGIIDPFTEDFSAFVFK
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHEEEE
IQANMNKAHRDRIAFMRICSGKFEAGMEANHVQGGKKIRLSQPQQMMAEERHIVDEAYAG
EECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCC
DIIGVFDPGIFSIGDTICKSNKKFAFEGIPTFAPEHFARVRQLDTMKRKQFIKGINQIAQ
CEEEECCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGAIQIFQEFNTGMEEIIVGVVGVLQFEVLEYRLKNEYGVDIRLEPLPYEHIRWIENPEE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHCCCCCC
IDVNRIVGTSDMKKIKDLKGNPLLVFANSWSVNMVLERNEGLKLSEFGRN
CCHHHEECCHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA