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The map label for this gene is pyrE [H]

Identifier: 238916486

GI number: 238916486

Start: 549234

End: 549965

Strand: Direct

Name: pyrE [H]

Synonym: EUBELI_00543

Alternate gene names: 238916486

Gene position: 549234-549965 (Clockwise)

Preceding gene: 238916485

Following gene: 238916487

Centisome position: 25.61

GC content: 42.08

Gene sequence:

>732_bases
ATGAAGCAGTGCCGCCGGACAGCGGGGCTGAATAAGGAGGATATAATGGAGCAGTATAAGCAGGAATTTATAGATTTCAT
GGTAGAGAGTGATGTGTTAAAGTTTGGAGATTTTACTTTAAAGAGTGGAAGAAAGTCACCTTTCTTCATGAACGCAGGTG
CGTATGTTACAGGAACACAGCTTGCAAGACTTGGTGAGTATTATGCAAAGGCTATTCATGAAACATATGGTGATGATTTT
GATGTACTGTTCGGACCAGCATATAAGGGAATTCCAATCAGCGTAGTTACAGCAGTTGCATATGCAAAGCTTTACGGCAA
GGAAGTAAGATACTGCTCAGACCGTAAGGAAGAGAAGGACCATGGTGCTGATAAGGGCGGATTTCTCGGAAGCAAGTTAA
AGGATGGCGACAGAGTCATTATGATAGAAGATGTAACAACATCTGGTAAGTCGATGGAAGAGACAGTTCCTAAGGTAAGA
GGGGCAGCAGATGTTACTATTGTAGGACTTATGGTTTCACTTAACCGTATGGAAGTAGGCCTTGGCGGAAAGGTGAGTGC
GCTTGAGGAGATTAAGGAAAGATACGGTTTCCCAACAAGCGCAATTGTATCTATGGCAGATGTTGTAGAACACCTTTACA
ATAAAGAGTGTGAGGGTAAGGTAGTTATTGATGATGCACTAAAAGCAGCAATTGACAAGTATTACGCAGAATATGGTGTT
AAGAACGCATAA

Upstream 100 bases:

>100_bases
ATAGGATTTTGATGAGCAGATTTTAATATGCAGATTTAATGTTCCAAAGACGCATTGAAACGAGAAAACATTTATGATAA
AATAAAAATCAATATTGAAT

Downstream 100 bases:

>100_bases
AACTGATGTTAATGGAGGGTTAACTATGGAAGAGAATCTTTATAAGAAATTAAAGTCAGTAGGAGCTTCTAATCTTGTAT
TTGGTATCCTGATAATACTC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKQCRRTAGLNKEDIMEQYKQEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGTQLARLGEYYAKAIHETYGDDF
DVLFGPAYKGIPISVVTAVAYAKLYGKEVRYCSDRKEEKDHGADKGGFLGSKLKDGDRVIMIEDVTTSGKSMEETVPKVR
GAADVTIVGLMVSLNRMEVGLGGKVSALEEIKERYGFPTSAIVSMADVVEHLYNKECEGKVVIDDALKAAIDKYYAEYGV
KNA

Sequences:

>Translated_243_residues
MKQCRRTAGLNKEDIMEQYKQEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGTQLARLGEYYAKAIHETYGDDF
DVLFGPAYKGIPISVVTAVAYAKLYGKEVRYCSDRKEEKDHGADKGGFLGSKLKDGDRVIMIEDVTTSGKSMEETVPKVR
GAADVTIVGLMVSLNRMEVGLGGKVSALEEIKERYGFPTSAIVSMADVVEHLYNKECEGKVVIDDALKAAIDKYYAEYGV
KNA
>Mature_243_residues
MKQCRRTAGLNKEDIMEQYKQEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGTQLARLGEYYAKAIHETYGDDF
DVLFGPAYKGIPISVVTAVAYAKLYGKEVRYCSDRKEEKDHGADKGGFLGSKLKDGDRVIMIEDVTTSGKSMEETVPKVR
GAADVTIVGLMVSLNRMEVGLGGKVSALEEIKERYGFPTSAIVSMADVVEHLYNKECEGKVVIDDALKAAIDKYYAEYGV
KNA

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790073, Length=225, Percent_Identity=43.1111111111111, Blast_Score=176, Evalue=9e-46,
Organism=Saccharomyces cerevisiae, GI6323530, Length=235, Percent_Identity=36.1702127659575, Blast_Score=146, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323927, Length=231, Percent_Identity=35.9307359307359, Blast_Score=142, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 26891; Mature: 26891

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQCRRTAGLNKEDIMEQYKQEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGTQ
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEECCCCEEHHHH
LARLGEYYAKAIHETYGDDFDVLFGPAYKGIPISVVTAVAYAKLYGKEVRYCSDRKEEKD
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HGADKGGFLGSKLKDGDRVIMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLNRMEVG
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEC
LGGKVSALEEIKERYGFPTSAIVSMADVVEHLYNKECEGKVVIDDALKAAIDKYYAEYGV
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCC
KNA
CCC
>Mature Secondary Structure
MKQCRRTAGLNKEDIMEQYKQEFIDFMVESDVLKFGDFTLKSGRKSPFFMNAGAYVTGTQ
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEECCCCEEHHHH
LARLGEYYAKAIHETYGDDFDVLFGPAYKGIPISVVTAVAYAKLYGKEVRYCSDRKEEKD
HHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
HGADKGGFLGSKLKDGDRVIMIEDVTTSGKSMEETVPKVRGAADVTIVGLMVSLNRMEVG
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHEEC
LGGKVSALEEIKERYGFPTSAIVSMADVVEHLYNKECEGKVVIDDALKAAIDKYYAEYGV
CCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHCC
KNA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA