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The map label for this gene is ispF

Identifier: 238916432

GI number: 238916432

Start: 496636

End: 497187

Strand: Direct

Name: ispF

Synonym: EUBELI_00488

Alternate gene names: 238916432

Gene position: 496636-497187 (Clockwise)

Preceding gene: 238916431

Following gene: 238916433

Centisome position: 23.16

GC content: 39.13

Gene sequence:

>552_bases
ATGAGAGTTGGAATGGGATATGATGTTCATAAACTGGTTAAAGATCGTAAGCTTATCATGGGCGGAGTTGAGATTCCATA
TGAATTAGGTCTGCTTGGACACTCTGATGCAGATGTGCTTCTTCATGCCATTATGGATGCATTACTGGGTGCTGCTGCAC
TTGGAGATATAGGAAAGCATTTCCCGGATACAGATGACAGATACAAGGGAATATCAAGTATCAAGCTTCTGGAAGAGGTT
GGAAAGAAGATAGATGAGGCTAATTATATTATAGAAAATATAGATGCTACTATTATTGCACAAAAACCTAAGATGCGCCC
ATATATTGATGATATGAGAAAGAATATCGCAGATGCTTTAAAGATTGATGTTGACAGAGTCAATGTTAAGGCTACTACAG
AAGAAGGACTTGGATTTACCGGAAGTGGAGAGGGTATTTCTTCACAGGCAATATGCTCACTTAATTCTGTTTCTAATTAT
ATATATGGTGCGGCTGATATGACAAACTGTAGATCTGACTGTGGTGGCTGCATGGGCTGTCAGAACAAATAG

Upstream 100 bases:

>100_bases
AGCTGGTGTCAATTTCACTTGATAATCCAGCTAAAATGTGCTTTACTTATATGGACTAGGTAAACAATTAATGTTGCAAT
CAAGATTAAGGAGGAGCTAT

Downstream 100 bases:

>100_bases
AACTGACAATACATTCGCAGCTTTAATTCATATAATATAATTAAGAATAGAAAAGTCAGGATTAACAGATTATGAAATAT
AGAGATTATGTAAAGCAGGA

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEV
GKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNSVSNY
IYGAADMTNCRSDCGGCMGCQNK

Sequences:

>Translated_183_residues
MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEV
GKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNSVSNY
IYGAADMTNCRSDCGGCMGCQNK
>Mature_183_residues
MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKHFPDTDDRYKGISSIKLLEEV
GKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADALKIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNSVSNY
IYGAADMTNCRSDCGGCMGCQNK

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=154, Percent_Identity=58.4415584415584, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_EUBE2 (C4Z3N3)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929949.1
- GeneID:   7957601
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00488
- OMA:   HKFGGEG
- ProtClustDB:   CLSK2506399
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 19786; Mature: 19786

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADAL
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
KIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNSVSNYIYGAADMTNCRSDCGGCMGC
HHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
QNK
CCC
>Mature Secondary Structure
MRVGMGYDVHKLVKDRKLIMGGVEIPYELGLLGHSDADVLLHAIMDALLGAAALGDIGKH
CCCCCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FPDTDDRYKGISSIKLLEEVGKKIDEANYIIENIDATIIAQKPKMRPYIDDMRKNIADAL
CCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHH
KIDVDRVNVKATTEEGLGFTGSGEGISSQAICSLNSVSNYIYGAADMTNCRSDCGGCMGC
HHHHHHEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCC
QNK
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA