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The map label for this gene is pfkA3 [H]

Identifier: 238916427

GI number: 238916427

Start: 492838

End: 493914

Strand: Direct

Name: pfkA3 [H]

Synonym: EUBELI_00483

Alternate gene names: 238916427

Gene position: 492838-493914 (Clockwise)

Preceding gene: 238916426

Following gene: 238916428

Centisome position: 22.98

GC content: 39.65

Gene sequence:

>1077_bases
ATGAAGATAGGTATGCTTACAAGTGGTGGAGACTGCCAGTCTCTTAATGCGGCAATGAGAGGTGTTGCCAAAACTTTGTA
TAATAATACAAATGATGTTGAAATATACGGATTTCTTGATGGTTACAAAGGAATGATTACTAAGAATTACAAACTGATGA
AAGAAGAGGATTTCAGTGGGATTCTCACAGAGGGAGGAACAATTCTTGGAACTTCCAGAACACCTTTCAAATATATTAAT
GAGCCTGATAAGGAAGGGCGCGATAAAGTTGCAGGCATGATTAAGACTTATAAGGATTTAAAACTCGACTGCCTGGTAAT
GCTTGGCGGAAACGGTTCACATAAGACTGCGGCTCTTTTATCGCAGAAGGGTTTAAATGTTGTTACTCTTCCTAAGACTA
TTGATAATGATATAGAAGGAACTGAGATGAGCTTTGGATTTCAGTCAGCTATAGATGTTGCGACAAGAACTATAGATGAG
ATTCATACAACGGCAGCCTCACACAGCAGAGTGTTTATTGTTGAGATTATGGGACATAAAACAGGCTGGCTGACATTGCA
TTCAGGAATTGCAGGCGGTGCTGATATCATACTTATACCTGAAATTCCATATAATGTTGATGAGGTGTTGAATACAATCA
GAAAGCGTGAAAAGCAGGGTAAGAAATTTACTATAATCGCAATGGCTGAGGGTGCAATATCAGATGAGGATGCAAAGCTG
TCAAAGAAAGAGCTGAAAGAAAAGAGAAAGACATATACATTTCCATCAGTAGCATATCAGTTGAGTGATGAGCTGCAGAA
AAAAGCCGGTCAGGAGGTAAGAGTAACAATTCCCGGACATGTTCAGCGTGGCGGAATTCCAAGTGCATCTGACAGAACAC
TGGCAACAAGAATAGGCGTTGAGGCTGCAGGACTTATATTAAGAAAAGATTATGGCAATATGGTATGCATCAAAGAAGGA
AAGTTCTCTAAGATACCACTTGAAGAGGTTGCAGGTAAAACGAAGATGGTTAACGTAGATAATGAACTTATAAGACAGGC
TGAAAGCTTAGGCATTAGTCTTGGCAGAAAGGCATAA

Upstream 100 bases:

>100_bases
TATTAATAATCCTAAGATTGATTCACAGGAAACACTATATGAAGAAAGCTATGAAGCGGCAGCCAGTGCAGAATAATTAA
GAAAATGGAGGCTAACAGGT

Downstream 100 bases:

>100_bases
GTGGTATATAATACAAAGCAAATGCTTCGGAAAGAAGGGAAATGCTTTGAAGAAATCAGACAGACTAAAGATAGTTATTC
TTGGAATAACCGGATTACTT

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase 3; Phosphohexokinase 3 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKIGMLTSGGDCQSLNAAMRGVAKTLYNNTNDVEIYGFLDGYKGMITKNYKLMKEEDFSGILTEGGTILGTSRTPFKYIN
EPDKEGRDKVAGMIKTYKDLKLDCLVMLGGNGSHKTAALLSQKGLNVVTLPKTIDNDIEGTEMSFGFQSAIDVATRTIDE
IHTTAASHSRVFIVEIMGHKTGWLTLHSGIAGGADIILIPEIPYNVDEVLNTIRKREKQGKKFTIIAMAEGAISDEDAKL
SKKELKEKRKTYTFPSVAYQLSDELQKKAGQEVRVTIPGHVQRGGIPSASDRTLATRIGVEAAGLILRKDYGNMVCIKEG
KFSKIPLEEVAGKTKMVNVDNELIRQAESLGISLGRKA

Sequences:

>Translated_358_residues
MKIGMLTSGGDCQSLNAAMRGVAKTLYNNTNDVEIYGFLDGYKGMITKNYKLMKEEDFSGILTEGGTILGTSRTPFKYIN
EPDKEGRDKVAGMIKTYKDLKLDCLVMLGGNGSHKTAALLSQKGLNVVTLPKTIDNDIEGTEMSFGFQSAIDVATRTIDE
IHTTAASHSRVFIVEIMGHKTGWLTLHSGIAGGADIILIPEIPYNVDEVLNTIRKREKQGKKFTIIAMAEGAISDEDAKL
SKKELKEKRKTYTFPSVAYQLSDELQKKAGQEVRVTIPGHVQRGGIPSASDRTLATRIGVEAAGLILRKDYGNMVCIKEG
KFSKIPLEEVAGKTKMVNVDNELIRQAESLGISLGRKA
>Mature_358_residues
MKIGMLTSGGDCQSLNAAMRGVAKTLYNNTNDVEIYGFLDGYKGMITKNYKLMKEEDFSGILTEGGTILGTSRTPFKYIN
EPDKEGRDKVAGMIKTYKDLKLDCLVMLGGNGSHKTAALLSQKGLNVVTLPKTIDNDIEGTEMSFGFQSAIDVATRTIDE
IHTTAASHSRVFIVEIMGHKTGWLTLHSGIAGGADIILIPEIPYNVDEVLNTIRKREKQGKKFTIIAMAEGAISDEDAKL
SKKELKEKRKTYTFPSVAYQLSDELQKKAGQEVRVTIPGHVQRGGIPSASDRTLATRIGVEAAGLILRKDYGNMVCIKEG
KFSKIPLEEVAGKTKMVNVDNELIRQAESLGISLGRKA

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=369, Percent_Identity=34.4173441734417, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI266453768, Length=369, Percent_Identity=33.0623306233062, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI266453748, Length=369, Percent_Identity=33.0623306233062, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4505749, Length=369, Percent_Identity=33.0623306233062, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI266453619, Length=369, Percent_Identity=33.0623306233062, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI48762920, Length=385, Percent_Identity=32.7272727272727, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=35.7366771159875, Blast_Score=191, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI25147584, Length=370, Percent_Identity=30.8108108108108, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17558788, Length=392, Percent_Identity=27.5510204081633, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI72003601, Length=320, Percent_Identity=26.5625, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321679, Length=383, Percent_Identity=34.9869451697128, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323861, Length=369, Percent_Identity=32.2493224932249, Blast_Score=159, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24652337, Length=369, Percent_Identity=31.9783197831978, Blast_Score=158, Evalue=7e-39,
Organism=Drosophila melanogaster, GI17647809, Length=369, Percent_Identity=31.9783197831978, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28573326, Length=369, Percent_Identity=31.7073170731707, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR022953
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 39096; Mature: 39096

Theoretical pI: Translated: 9.15; Mature: 9.15

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGMLTSGGDCQSLNAAMRGVAKTLYNNTNDVEIYGFLDGYKGMITKNYKLMKEEDFSG
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCEEEEECCCCCE
ILTEGGTILGTSRTPFKYINEPDKEGRDKVAGMIKTYKDLKLDCLVMLGGNGSHKTAALL
EEECCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
SQKGLNVVTLPKTIDNDIEGTEMSFGFQSAIDVATRTIDEIHTTAASHSRVFIVEIMGHK
HHCCCCEEECCHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
TGWLTLHSGIAGGADIILIPEIPYNVDEVLNTIRKREKQGKKFTIIAMAEGAISDEDAKL
CCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
SKKELKEKRKTYTFPSVAYQLSDELQKKAGQEVRVTIPGHVQRGGIPSASDRTLATRIGV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCEEHHHHCC
EAAGLILRKDYGNMVCIKEGKFSKIPLEEVAGKTKMVNVDNELIRQAESLGISLGRKA
HHHCEEEEECCCCEEEEECCCCCCCCHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKIGMLTSGGDCQSLNAAMRGVAKTLYNNTNDVEIYGFLDGYKGMITKNYKLMKEEDFSG
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCEEEEECCCCCE
ILTEGGTILGTSRTPFKYINEPDKEGRDKVAGMIKTYKDLKLDCLVMLGGNGSHKTAALL
EEECCCEEEECCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHH
SQKGLNVVTLPKTIDNDIEGTEMSFGFQSAIDVATRTIDEIHTTAASHSRVFIVEIMGHK
HHCCCCEEECCHHHCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCC
TGWLTLHSGIAGGADIILIPEIPYNVDEVLNTIRKREKQGKKFTIIAMAEGAISDEDAKL
CCEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCHHH
SKKELKEKRKTYTFPSVAYQLSDELQKKAGQEVRVTIPGHVQRGGIPSASDRTLATRIGV
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCEEHHHHCC
EAAGLILRKDYGNMVCIKEGKFSKIPLEEVAGKTKMVNVDNELIRQAESLGISLGRKA
HHHCEEEEECCCCEEEEECCCCCCCCHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]