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The map label for this gene is purB [H]

Identifier: 238916407

GI number: 238916407

Start: 473378

End: 474826

Strand: Direct

Name: purB [H]

Synonym: EUBELI_00461

Alternate gene names: 238916407

Gene position: 473378-474826 (Clockwise)

Preceding gene: 238916406

Following gene: 238916408

Centisome position: 22.08

GC content: 40.92

Gene sequence:

>1449_bases
ATGAAAGGCGGATACGAGATGAACGACAGATATCAGACACCACTTGCAGAAAGATACGCAAGCAAGGAAATGCAGTACAT
TTTCTCTCCTGACATGAAGTTTAAGACATGGAGAAGACTCTGGATTGCATTAGCAGAGACAGAGCATGAACTTGGACTTC
CTATTACACAGGAGCAGATTGATGAGTTAAAGGCTCACAAGGATGATATTAACTATGAGGATGCCAAGAGACGAGAGAAG
GAAGTAAGACATGATGTTATGTCTCATGTATATGCTTACGGACTTCAGTGCCCTAAGGCTAAAGGTATCATACATCTTGG
AGCAACTTCATGCTATGTTGGTGATAATACTGATATCATCATCATGACAGAAGGCTTAAAGCTTGTTCGTAAGAAGCTTG
TTAATGTTATTGATGAGCTTTCTAAGTTCGCAGATAAATATAAGGCACAGCCTACACTGGCTTTCACACATTTTCAGCCA
GCACAGCCAACTACAGTTGGTAAGAGAGCTACATTATGGCTCAATGAATTAGTTATGGACCTTGAAGACCTTGATTATGT
ACTCTCTACAATGAAGCTTTTAGGCTGCAAGGGTACAACAGGTACACAGGCTTCTTTCCTTGAACTTTTCAATGGAGATC
AGGATAAGATCCGCCAGATTGACGGAAAGATTGCAGAAAAGATGGGATTTGACGCATGTGTTCCAGTTTCAGGACAGACA
TATTCAAGAAAAGTTGATACAAGAGTGCTTAATGTACTTGCAGGAATTGCTGCGAGCGCACACAAGTTCTCTAATGATAT
CAGATTATTACAGCATCTCAAGGAAGTTGAAGAGCCATTTGAAAAGTCACAGATTGGTTCATCAGCTATGGCTTACAAGC
GTAATCCTATGAGAAGTGAGCGTATGGCTTCACTTGCAGATTTCGTTATCAGTGATGCACTTAACCCTGCTATTGTTTCA
TCTACACAGTGGTTTGAGAGAACACTTGATGATTCAGCTAACAAGAGAATGTCAGTTCCAGAGGGATTTCTCGCTGTTGA
CGGAATCCTTGACCTCTACTTGAATGTTGTTGACGGACTTGTTGTATATGACAAGGTTATCATCAAACATATGATGGCAG
AGCTTCCATTCATGGCAACTGAGAATATTATGATGGACGCTGTTAAGGCTGGTGGAGACAGACAGGAGCTTCATGAGAAG
ATCAGAGAGCTTTCTATGATAGCAGGAAAGAGAGTTAAGCAGGAAGGTCTTGATAATAACCTTCTTGAGCTTATTGCAGC
AGAGCCAATGTTTGGTGTTACTCTTGAAGAGCTACAGTCAAAGCTTGACCCTATGAAGTATGTTGGCCGTTCTAAAGAAC
AGGTTGACGAATATCTTGCTGATGTTATCAAGCCTATCCTTGATGCTAACAAGGAAGAACTTGGTATGAAGGCAGAGATT
AATGTTTAA

Upstream 100 bases:

>100_bases
AAGAATATAAAATAAAGGCATAAATTGCAAACGCAGTTCTGCTATGATATACTTAATCTGTATATAGCCCTGCGATGGCT
GAATAATGAATTTTTGATAT

Downstream 100 bases:

>100_bases
TTAATAATTTGGAGGTGTTATAACATGAAGGTTGTATTAGTTAATGGCAGCAGAAGAGATGCAGGCTGTACATATACAGC
ACTTTCTGTCGTTGCTAAGG

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKGGYEMNDRYQTPLAERYASKEMQYIFSPDMKFKTWRRLWIALAETEHELGLPITQEQIDELKAHKDDINYEDAKRREK
EVRHDVMSHVYAYGLQCPKAKGIIHLGATSCYVGDNTDIIIMTEGLKLVRKKLVNVIDELSKFADKYKAQPTLAFTHFQP
AQPTTVGKRATLWLNELVMDLEDLDYVLSTMKLLGCKGTTGTQASFLELFNGDQDKIRQIDGKIAEKMGFDACVPVSGQT
YSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEVEEPFEKSQIGSSAMAYKRNPMRSERMASLADFVISDALNPAIVS
STQWFERTLDDSANKRMSVPEGFLAVDGILDLYLNVVDGLVVYDKVIIKHMMAELPFMATENIMMDAVKAGGDRQELHEK
IRELSMIAGKRVKQEGLDNNLLELIAAEPMFGVTLEELQSKLDPMKYVGRSKEQVDEYLADVIKPILDANKEELGMKAEI
NV

Sequences:

>Translated_482_residues
MKGGYEMNDRYQTPLAERYASKEMQYIFSPDMKFKTWRRLWIALAETEHELGLPITQEQIDELKAHKDDINYEDAKRREK
EVRHDVMSHVYAYGLQCPKAKGIIHLGATSCYVGDNTDIIIMTEGLKLVRKKLVNVIDELSKFADKYKAQPTLAFTHFQP
AQPTTVGKRATLWLNELVMDLEDLDYVLSTMKLLGCKGTTGTQASFLELFNGDQDKIRQIDGKIAEKMGFDACVPVSGQT
YSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEVEEPFEKSQIGSSAMAYKRNPMRSERMASLADFVISDALNPAIVS
STQWFERTLDDSANKRMSVPEGFLAVDGILDLYLNVVDGLVVYDKVIIKHMMAELPFMATENIMMDAVKAGGDRQELHEK
IRELSMIAGKRVKQEGLDNNLLELIAAEPMFGVTLEELQSKLDPMKYVGRSKEQVDEYLADVIKPILDANKEELGMKAEI
NV
>Mature_482_residues
MKGGYEMNDRYQTPLAERYASKEMQYIFSPDMKFKTWRRLWIALAETEHELGLPITQEQIDELKAHKDDINYEDAKRREK
EVRHDVMSHVYAYGLQCPKAKGIIHLGATSCYVGDNTDIIIMTEGLKLVRKKLVNVIDELSKFADKYKAQPTLAFTHFQP
AQPTTVGKRATLWLNELVMDLEDLDYVLSTMKLLGCKGTTGTQASFLELFNGDQDKIRQIDGKIAEKMGFDACVPVSGQT
YSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEVEEPFEKSQIGSSAMAYKRNPMRSERMASLADFVISDALNPAIVS
STQWFERTLDDSANKRMSVPEGFLAVDGILDLYLNVVDGLVVYDKVIIKHMMAELPFMATENIMMDAVKAGGDRQELHEK
IRELSMIAGKRVKQEGLDNNLLELIAAEPMFGVTLEELQSKLDPMKYVGRSKEQVDEYLADVIKPILDANKEELGMKAEI
NV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=478, Percent_Identity=54.3933054393305, Blast_Score=537, Evalue=1e-153,
Organism=Homo sapiens, GI183227688, Length=393, Percent_Identity=57.7608142493639, Blast_Score=478, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI17508577, Length=463, Percent_Identity=38.6609071274298, Blast_Score=337, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI32564234, Length=344, Percent_Identity=43.3139534883721, Blast_Score=294, Evalue=6e-80,
Organism=Saccharomyces cerevisiae, GI6323391, Length=457, Percent_Identity=54.0481400437637, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24647570, Length=474, Percent_Identity=52.5316455696203, Blast_Score=502, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54489; Mature: 54489

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGGYEMNDRYQTPLAERYASKEMQYIFSPDMKFKTWRRLWIALAETEHELGLPITQEQI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHH
DELKAHKDDINYEDAKRREKEVRHDVMSHVYAYGLQCPKAKGIIHLGATSCYVGDNTDII
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEE
IMTEGLKLVRKKLVNVIDELSKFADKYKAQPTLAFTHFQPAQPTTVGKRATLWLNELVMD
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
LEDLDYVLSTMKLLGCKGTTGTQASFLELFNGDQDKIRQIDGKIAEKMGFDACVPVSGQT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCC
YSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEVEEPFEKSQIGSSAMAYKRNPMRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHH
RMASLADFVISDALNPAIVSSTQWFERTLDDSANKRMSVPEGFLAVDGILDLYLNVVDGL
HHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VVYDKVIIKHMMAELPFMATENIMMDAVKAGGDRQELHEKIRELSMIAGKRVKQEGLDNN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLELIAAEPMFGVTLEELQSKLDPMKYVGRSKEQVDEYLADVIKPILDANKEELGMKAEI
HHHHHHCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEC
NV
CC
>Mature Secondary Structure
MKGGYEMNDRYQTPLAERYASKEMQYIFSPDMKFKTWRRLWIALAETEHELGLPITQEQI
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCCCCHHHH
DELKAHKDDINYEDAKRREKEVRHDVMSHVYAYGLQCPKAKGIIHLGATSCYVGDNTDII
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEE
IMTEGLKLVRKKLVNVIDELSKFADKYKAQPTLAFTHFQPAQPTTVGKRATLWLNELVMD
EEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
LEDLDYVLSTMKLLGCKGTTGTQASFLELFNGDQDKIRQIDGKIAEKMGFDACVPVSGQT
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEECCCCCC
YSRKVDTRVLNVLAGIAASAHKFSNDIRLLQHLKEVEEPFEKSQIGSSAMAYKRNPMRSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHH
RMASLADFVISDALNPAIVSSTQWFERTLDDSANKRMSVPEGFLAVDGILDLYLNVVDGL
HHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VVYDKVIIKHMMAELPFMATENIMMDAVKAGGDRQELHEKIRELSMIAGKRVKQEGLDNN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
LLELIAAEPMFGVTLEELQSKLDPMKYVGRSKEQVDEYLADVIKPILDANKEELGMKAEI
HHHHHHCCCCCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEC
NV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]