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The map label for this gene is gatB

Identifier: 238916394

GI number: 238916394

Start: 458191

End: 459642

Strand: Direct

Name: gatB

Synonym: EUBELI_00448

Alternate gene names: 238916394

Gene position: 458191-459642 (Clockwise)

Preceding gene: 238916393

Following gene: 238916395

Centisome position: 21.37

GC content: 40.98

Gene sequence:

>1452_bases
ATGAAAGATTATGAAGTTGTAATCGGACTTGAAGTCCATGTTGAATTAGCTACTAAGACAAAGATTTTCTGCGGATGCTC
AACTGAGTTTGGTGGAGCACCTAACTCACATACTTGCCCTGTGTGTACAGGTATGCCGGGTTCTCTTCCTGTTCTTAATA
AGAAGGTTGTTGAATATGCAGCAGCAGTAGGACTTGCAACTAACTGTACAATTACACAGGACTGCAAGTTTGACAGAAAG
AATTATTTCTATCCTGATAATCCACAGAATTATCAGATTTCACAGCTTTATCTTCCAATCTGCCGTAACGGACATGTTGA
TGTTACATTATCAGATGGAACAGAAAAGACAATAAGAATCCACGAGATTCATATGGAAGAGGATGCAGGAAAGCTTGTTC
ATGATGAGTGGGAAGGTGTGTCATATGTAGATTATAACCGTTCAGGTGTGCCACTTATCGAAATTGTTTCTGAACCGGAT
ATGCGTTCAGCAGAAGAAGTTATTGCTTACCTTACTAAGTTAAGAACAACAATCCAGTATCTTGGTGCTTCTGACTGCAA
GCTTCAGGAAGGTTCTATGAGAGCAGATGTCAACCTCTCTGTAAGAGAGCGTGGTTCAAACGAGTTCGGAACAAGAACAG
AAACTAAGAACCTCAATTCATTTGCAGCGATTGAAAGAGCAATTAAGGCCGAGACAGCTAGACAGATTGACTTAATCGAA
GCAGGCGAGAAGGTTGTGCAGGAAACAAGAAGATGGAATGATGACAAGGAATATTCATATGCTATGCGTTCAAAGGAAGA
TGCGCAGGATTACAGATATTTCCCAGATCCAGACCTTGTTCCAATTCACATCAGTGATGAGTATTTAGCAGAACTTAAGG
CAAAACAGCCAGAGTTCAAGGACGAGAAGAAGGCACGTTATATTGAAGAATTCGGACTTCCTGAATATGATGCAGAGATT
CTTACAGATTCTAAGAAGTTCACAGAAATCTTTGAGGAAGCTACAGCCATCTGCAATCAGCCAAAGAAGGTTTCCAACTG
GATTATGGGCGAGACAATGCGACTCATGAAGGAAGAATCAGCTAAGACTGGAAGAGAATTCAGAGCCGAGGAATTAACAT
TTTCACCTGAAAGCCTTGCCAAGATTATTACTCTTGTTGAAAAGAAGGAAATCAACAATGCAGCAGCTAAAGAAATATTC
GAGCATGTATTTGCTGAAAATGTTGATGTTGACGCATATGTTGAGGAACACGGATTAAAGCAGGTTAATGATGAAGGCGC
GCTCAGAGCAACAGTCGAGAAGGTTATCGCTGATAATCCACAGTCAGTTGCTGATTATAAGGGCGGTAAGAAGCAGGCAA
TCGGTTATCTTGTAGGCCAGACTATGAAGGCAATGCAGGGAAAAGCTAATCCGGGAATGGTTAATGCTCTTTTACAGGAG
CTTTTAAAGTAA

Upstream 100 bases:

>100_bases
ACAGCTTATTGGCGACTGCTTTAAAGAGAATAATATTATCCGTGCGGGCTACGCTTATGAGCAGGCACGAGGAAGATTTG
AATAGGAGGGAGGCATAAGT

Downstream 100 bases:

>100_bases
CACATGAACTTAAAACTAGAAGGAGATGTGGAATGAAAAAAGTTAATTTTGATGTAAAACTCCCAGCATTTGTGGAGAGA
ATAGTATATGTTGATAACTA

Product: aspartyl-tRNA(Asn)/glutamyl-tRNA (Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKDYEVVIGLEVHVELATKTKIFCGCSTEFGGAPNSHTCPVCTGMPGSLPVLNKKVVEYAAAVGLATNCTITQDCKFDRK
NYFYPDNPQNYQISQLYLPICRNGHVDVTLSDGTEKTIRIHEIHMEEDAGKLVHDEWEGVSYVDYNRSGVPLIEIVSEPD
MRSAEEVIAYLTKLRTTIQYLGASDCKLQEGSMRADVNLSVRERGSNEFGTRTETKNLNSFAAIERAIKAETARQIDLIE
AGEKVVQETRRWNDDKEYSYAMRSKEDAQDYRYFPDPDLVPIHISDEYLAELKAKQPEFKDEKKARYIEEFGLPEYDAEI
LTDSKKFTEIFEEATAICNQPKKVSNWIMGETMRLMKEESAKTGREFRAEELTFSPESLAKIITLVEKKEINNAAAKEIF
EHVFAENVDVDAYVEEHGLKQVNDEGALRATVEKVIADNPQSVADYKGGKKQAIGYLVGQTMKAMQGKANPGMVNALLQE
LLK

Sequences:

>Translated_483_residues
MKDYEVVIGLEVHVELATKTKIFCGCSTEFGGAPNSHTCPVCTGMPGSLPVLNKKVVEYAAAVGLATNCTITQDCKFDRK
NYFYPDNPQNYQISQLYLPICRNGHVDVTLSDGTEKTIRIHEIHMEEDAGKLVHDEWEGVSYVDYNRSGVPLIEIVSEPD
MRSAEEVIAYLTKLRTTIQYLGASDCKLQEGSMRADVNLSVRERGSNEFGTRTETKNLNSFAAIERAIKAETARQIDLIE
AGEKVVQETRRWNDDKEYSYAMRSKEDAQDYRYFPDPDLVPIHISDEYLAELKAKQPEFKDEKKARYIEEFGLPEYDAEI
LTDSKKFTEIFEEATAICNQPKKVSNWIMGETMRLMKEESAKTGREFRAEELTFSPESLAKIITLVEKKEINNAAAKEIF
EHVFAENVDVDAYVEEHGLKQVNDEGALRATVEKVIADNPQSVADYKGGKKQAIGYLVGQTMKAMQGKANPGMVNALLQE
LLK
>Mature_483_residues
MKDYEVVIGLEVHVELATKTKIFCGCSTEFGGAPNSHTCPVCTGMPGSLPVLNKKVVEYAAAVGLATNCTITQDCKFDRK
NYFYPDNPQNYQISQLYLPICRNGHVDVTLSDGTEKTIRIHEIHMEEDAGKLVHDEWEGVSYVDYNRSGVPLIEIVSEPD
MRSAEEVIAYLTKLRTTIQYLGASDCKLQEGSMRADVNLSVRERGSNEFGTRTETKNLNSFAAIERAIKAETARQIDLIE
AGEKVVQETRRWNDDKEYSYAMRSKEDAQDYRYFPDPDLVPIHISDEYLAELKAKQPEFKDEKKARYIEEFGLPEYDAEI
LTDSKKFTEIFEEATAICNQPKKVSNWIMGETMRLMKEESAKTGREFRAEELTFSPESLAKIITLVEKKEINNAAAKEIF
EHVFAENVDVDAYVEEHGLKQVNDEGALRATVEKVIADNPQSVADYKGGKKQAIGYLVGQTMKAMQGKANPGMVNALLQE
LLK

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=503, Percent_Identity=33.7972166998012, Blast_Score=296, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI115534094, Length=313, Percent_Identity=35.7827476038339, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=511, Percent_Identity=30.5283757338552, Blast_Score=223, Evalue=7e-59,
Organism=Drosophila melanogaster, GI45550808, Length=499, Percent_Identity=31.2625250501002, Blast_Score=267, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_EUBE2 (C4Z3J5)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929911.1
- GeneID:   7957563
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00448
- OMA:   LRYLGTC
- ProtClustDB:   CLSK2506101
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 54427; Mature: 54427

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYEVVIGLEVHVELATKTKIFCGCSTEFGGAPNSHTCPVCTGMPGSLPVLNKKVVEYA
CCCCEEEEEEEEEEEEEECEEEEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
AAVGLATNCTITQDCKFDRKNYFYPDNPQNYQISQLYLPICRNGHVDVTLSDGTEKTIRI
HHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCEEEEECCCCCCEEEE
HEIHMEEDAGKLVHDEWEGVSYVDYNRSGVPLIEIVSEPDMRSAEEVIAYLTKLRTTIQY
EEEEECCCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGASDCKLQEGSMRADVNLSVRERGSNEFGTRTETKNLNSFAAIERAIKAETARQIDLIE
HCCCCCEECCCCEEECEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AGEKVVQETRRWNDDKEYSYAMRSKEDAQDYRYFPDPDLVPIHISDEYLAELKAKQPEFK
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCC
DEKKARYIEEFGLPEYDAEILTDSKKFTEIFEEATAICNQPKKVSNWIMGETMRLMKEES
HHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AKTGREFRAEELTFSPESLAKIITLVEKKEINNAAAKEIFEHVFAENVDVDAYVEEHGLK
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCH
QVNDEGALRATVEKVIADNPQSVADYKGGKKQAIGYLVGQTMKAMQGKANPGMVNALLQE
HCCCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLK
HHC
>Mature Secondary Structure
MKDYEVVIGLEVHVELATKTKIFCGCSTEFGGAPNSHTCPVCTGMPGSLPVLNKKVVEYA
CCCCEEEEEEEEEEEEEECEEEEEECCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHH
AAVGLATNCTITQDCKFDRKNYFYPDNPQNYQISQLYLPICRNGHVDVTLSDGTEKTIRI
HHHCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEEEEHHCCCCEEEEECCCCCCEEEE
HEIHMEEDAGKLVHDEWEGVSYVDYNRSGVPLIEIVSEPDMRSAEEVIAYLTKLRTTIQY
EEEEECCCCCCHHHHCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
LGASDCKLQEGSMRADVNLSVRERGSNEFGTRTETKNLNSFAAIERAIKAETARQIDLIE
HCCCCCEECCCCEEECEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AGEKVVQETRRWNDDKEYSYAMRSKEDAQDYRYFPDPDLVPIHISDEYLAELKAKQPEFK
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHCCCCCCCCEEEEEECHHHHHHHHHCCCCCC
DEKKARYIEEFGLPEYDAEILTDSKKFTEIFEEATAICNQPKKVSNWIMGETMRLMKEES
HHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AKTGREFRAEELTFSPESLAKIITLVEKKEINNAAAKEIFEHVFAENVDVDAYVEEHGLK
HHCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHCCCH
QVNDEGALRATVEKVIADNPQSVADYKGGKKQAIGYLVGQTMKAMQGKANPGMVNALLQE
HCCCCCHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LLK
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA