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The map label for this gene is gatA

Identifier: 238916393

GI number: 238916393

Start: 456730

End: 458175

Strand: Direct

Name: gatA

Synonym: EUBELI_00447

Alternate gene names: 238916393

Gene position: 456730-458175 (Clockwise)

Preceding gene: 238916392

Following gene: 238916394

Centisome position: 21.3

GC content: 41.08

Gene sequence:

>1446_bases
ATGAGTTTATTAGATTATACAGCTGTTGAACTGGCTGCTAAGATTAAAGCGGGTGAGACAACAGCAACAGAGGCTATGGA
AGCTGTTATTGCACAGATTGAAAAATCTGAAGATGAGCTTAACTGTTATGTCACATTTGACAAGGAAAAGGCTCTTGCAG
CAGCAAAGAAAGCAGATGAGGATATCAAGGCAGGTAAGCTTACAGGACCTTTAGCCGGAGTACCATTTGCAATCAAAGAT
AATATGTGTACAGAGGGCATGCTTACTACATGCTCATCAAAGATACTTGGTAATTTCGTTCCAACTTACACAGCAGACGC
TGTAGAAAGACTTCAGAATGCAGGAGCTGTAATTATCGGAAAGACTAACATGGATGAATTCGCGATGGGTTCTACAACTG
AGACAAGTGCATTTGGTGCAACTAAGAATCCACGCAATCCTGAGCATGTACCTGGTGGTTCATCAGGTGGTTCAGCAGCA
GCAGTAGCAGCTAATGAGTGTTTTGCAGCACTTGGTTCTGATACAGGTGGATCAATAAGACAGCCAGCTTCTTACTGTGG
TGTTGTAGGCTTAAAGCCAACTTATGGAACAGTTTCACGTTACGGACTTATCGCTTATGGTTCATCATTAGACCAGATTG
GACCTTTATGTAAGGATGTAACTGACTGTGCAACTATCATGGAAGCTATCGCAGGCAAGGACGATAAGGATTCAACATCT
ATCGGAAGGGATGATTATTCATTTACAAATGCATTAGTTGATGATGTTAAGGGAATGAAAATCGGTATTCCAAGAGATTA
TTTTGGAGAAGGTCTTGACCCAGAGGTTAAGGAAGCTGTTCTTAACGCTGCCAAAGTTCTTGAGAGCAAGGGCGCAATCG
TTGAAGAGTTTGATTTAAGCCTTGTTGAGTACGCAATTCCTACTTATTATACAATCGCAGCAGCAGAAGCAAGCTCTAAC
CTTGAAAGATTCGATGGTGTTAAATATGGATACAGAACTAAAGAATACGAAGGACTCCACAACATGTACAAGAAGACACG
TTCAGAGGGCTTTGGACCAGAGGTTAAGAGAAGAATTATGCTCGGTTCTTTCGTATTAAGTTCAGGATATTATGACGCTT
ATTATCTTAAGGCATTAAGAGTTAAGGCTCTTATTAAGAAAGCATTTGATGAAGCATTTGCAAAATACGATGTAATTCTT
GGACCTGTTGCACCTACAACAGCACCAAAGCTTGGTTCATCACTTTCTGACCCTATTAAAATGTATCTTGGAGATATCTA
TACTATTTCTGTAAATCTTGCCGGACTTCCGGGACTTTCTGTTCCATGCGGTAAGGACAAGAATGGTCTTCCAATCGGCT
TACAGCTTATTGGCGACTGCTTTAAAGAGAATAATATTATCCGTGCGGGCTACGCTTATGAGCAGGCACGAGGAAGATTT
GAATAG

Upstream 100 bases:

>100_bases
CTAATGGTGACGGAAGCGAGGCAACTCTTGCTAATGCACCTGAATGTAATGAATCAGGATTTATTGTACCTAAAACTGTT
GAATCATAGAAAGGACGCAT

Downstream 100 bases:

>100_bases
GAGGGAGGCATAAGTATGAAAGATTATGAAGTTGTAATCGGACTTGAAGTCCATGTTGAATTAGCTACTAAGACAAAGAT
TTTCTGCGGATGCTCAACTG

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSLLDYTAVELAAKIKAGETTATEAMEAVIAQIEKSEDELNCYVTFDKEKALAAAKKADEDIKAGKLTGPLAGVPFAIKD
NMCTEGMLTTCSSKILGNFVPTYTADAVERLQNAGAVIIGKTNMDEFAMGSTTETSAFGATKNPRNPEHVPGGSSGGSAA
AVAANECFAALGSDTGGSIRQPASYCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPLCKDVTDCATIMEAIAGKDDKDSTS
IGRDDYSFTNALVDDVKGMKIGIPRDYFGEGLDPEVKEAVLNAAKVLESKGAIVEEFDLSLVEYAIPTYYTIAAAEASSN
LERFDGVKYGYRTKEYEGLHNMYKKTRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDEAFAKYDVIL
GPVAPTTAPKLGSSLSDPIKMYLGDIYTISVNLAGLPGLSVPCGKDKNGLPIGLQLIGDCFKENNIIRAGYAYEQARGRF
E

Sequences:

>Translated_481_residues
MSLLDYTAVELAAKIKAGETTATEAMEAVIAQIEKSEDELNCYVTFDKEKALAAAKKADEDIKAGKLTGPLAGVPFAIKD
NMCTEGMLTTCSSKILGNFVPTYTADAVERLQNAGAVIIGKTNMDEFAMGSTTETSAFGATKNPRNPEHVPGGSSGGSAA
AVAANECFAALGSDTGGSIRQPASYCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPLCKDVTDCATIMEAIAGKDDKDSTS
IGRDDYSFTNALVDDVKGMKIGIPRDYFGEGLDPEVKEAVLNAAKVLESKGAIVEEFDLSLVEYAIPTYYTIAAAEASSN
LERFDGVKYGYRTKEYEGLHNMYKKTRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDEAFAKYDVIL
GPVAPTTAPKLGSSLSDPIKMYLGDIYTISVNLAGLPGLSVPCGKDKNGLPIGLQLIGDCFKENNIIRAGYAYEQARGRF
E
>Mature_480_residues
SLLDYTAVELAAKIKAGETTATEAMEAVIAQIEKSEDELNCYVTFDKEKALAAAKKADEDIKAGKLTGPLAGVPFAIKDN
MCTEGMLTTCSSKILGNFVPTYTADAVERLQNAGAVIIGKTNMDEFAMGSTTETSAFGATKNPRNPEHVPGGSSGGSAAA
VAANECFAALGSDTGGSIRQPASYCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPLCKDVTDCATIMEAIAGKDDKDSTSI
GRDDYSFTNALVDDVKGMKIGIPRDYFGEGLDPEVKEAVLNAAKVLESKGAIVEEFDLSLVEYAIPTYYTIAAAEASSNL
ERFDGVKYGYRTKEYEGLHNMYKKTRSEGFGPEVKRRIMLGSFVLSSGYYDAYYLKALRVKALIKKAFDEAFAKYDVILG
PVAPTTAPKLGSSLSDPIKMYLGDIYTISVNLAGLPGLSVPCGKDKNGLPIGLQLIGDCFKENNIIRAGYAYEQARGRFE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=40.2414486921529, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=25.7606490872211, Blast_Score=136, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI17543272, Length=450, Percent_Identity=37.5555555555556, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=27.3092369477912, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17556264, Length=297, Percent_Identity=28.6195286195286, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71990152, Length=321, Percent_Identity=26.4797507788162, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=306, Percent_Identity=26.4705882352941, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=27.7070063694268, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=314, Percent_Identity=27.7070063694268, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=327, Percent_Identity=25.3822629969419, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=459, Percent_Identity=34.4226579520697, Blast_Score=226, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=420, Percent_Identity=31.1904761904762, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320448, Length=238, Percent_Identity=30.672268907563, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648113, Length=490, Percent_Identity=39.7959183673469, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=27.1084337349398, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=27.1084337349398, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=27.1084337349398, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=27.1084337349398, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24644968, Length=510, Percent_Identity=26.8627450980392, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI161078093, Length=500, Percent_Identity=27, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24648435, Length=483, Percent_Identity=25.4658385093168, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=483, Percent_Identity=25.4658385093168, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI45550774, Length=483, Percent_Identity=25.4658385093168, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356731, Length=472, Percent_Identity=24.5762711864407, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24648441, Length=408, Percent_Identity=24.7549019607843, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648439, Length=408, Percent_Identity=24.7549019607843, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_EUBE2 (C4Z3J4)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929910.1
- GeneID:   7957562
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00447
- OMA:   ICTVIAN
- ProtClustDB:   CLSK2506100
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51314; Mature: 51183

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 79-79 ACT_SITE 154-154 ACT_SITE 178-178

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLDYTAVELAAKIKAGETTATEAMEAVIAQIEKSEDELNCYVTFDKEKALAAAKKADE
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCC
DIKAGKLTGPLAGVPFAIKDNMCTEGMLTTCSSKILGNFVPTYTADAVERLQNAGAVIIG
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEE
KTNMDEFAMGSTTETSAFGATKNPRNPEHVPGGSSGGSAAAVAANECFAALGSDTGGSIR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCC
QPASYCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPLCKDVTDCATIMEAIAGKDDKDSTS
CCHHHHCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IGRDDYSFTNALVDDVKGMKIGIPRDYFGEGLDPEVKEAVLNAAKVLESKGAIVEEFDLS
CCCCCHHHHHHHHHHCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
LVEYAIPTYYTIAAAEASSNLERFDGVKYGYRTKEYEGLHNMYKKTRSEGFGPEVKRRIM
HHHHHCCCEEEEEEHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGSFVLSSGYYDAYYLKALRVKALIKKAFDEAFAKYDVILGPVAPTTAPKLGSSLSDPIK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCCCCHHHH
MYLGDIYTISVNLAGLPGLSVPCGKDKNGLPIGLQLIGDCFKENNIIRAGYAYEQARGRF
HHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHCCCC
E
C
>Mature Secondary Structure 
SLLDYTAVELAAKIKAGETTATEAMEAVIAQIEKSEDELNCYVTFDKEKALAAAKKADE
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHCCC
DIKAGKLTGPLAGVPFAIKDNMCTEGMLTTCSSKILGNFVPTYTADAVERLQNAGAVIIG
HHCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEE
KTNMDEFAMGSTTETSAFGATKNPRNPEHVPGGSSGGSAAAVAANECFAALGSDTGGSIR
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCCCCC
QPASYCGVVGLKPTYGTVSRYGLIAYGSSLDQIGPLCKDVTDCATIMEAIAGKDDKDSTS
CCHHHHCCCCCCCCCCCHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
IGRDDYSFTNALVDDVKGMKIGIPRDYFGEGLDPEVKEAVLNAAKVLESKGAIVEEFDLS
CCCCCHHHHHHHHHHCCCCEECCCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
LVEYAIPTYYTIAAAEASSNLERFDGVKYGYRTKEYEGLHNMYKKTRSEGFGPEVKRRIM
HHHHHCCCEEEEEEHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHH
LGSFVLSSGYYDAYYLKALRVKALIKKAFDEAFAKYDVILGPVAPTTAPKLGSSLSDPIK
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHCCCCCHHHH
MYLGDIYTISVNLAGLPGLSVPCGKDKNGLPIGLQLIGDCFKENNIIRAGYAYEQARGRF
HHHCCEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA