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The map label for this gene is aspS [H]

Identifier: 238916390

GI number: 238916390

Start: 454129

End: 455874

Strand: Direct

Name: aspS [H]

Synonym: EUBELI_00444

Alternate gene names: 238916390

Gene position: 454129-455874 (Clockwise)

Preceding gene: 238916389

Following gene: 238916392

Centisome position: 21.18

GC content: 40.55

Gene sequence:

>1746_bases
ATGGCAAACATGTACAGAGATGTTAACTTAAGTGAAGTTAATGAAAGTTACATCGGCAAGGAGTTAAAGGTTGCAGGATG
GGTTGAGAACATCCGTGACCACGGAGGAGTTTCATTTATCGATTTAAGAGATATGTATGGAGTACTTCAGGTAGTACTTC
GTGATACAACATTACTTGATGGTATTCATAAGGAAGAGTGTATCGCTATCGAGGGTATTATGGAGCAGAGAGATGAGGAA
ACTTATAATCCTAAGATTCCTTCAGGAACAATCGAGCTTGAAGCTAAGAAGATTACAATACTTGGACAGGTATACCAGCA
GCTTCCATTTGAAGTACAGACTTCTAAGGAAGTAAGAGAGGATGTACGTCTTAAGTACAGATATCTTGATTTAAGAAACC
AGAAGGTTAAGGATAACATGATATTCAGGTCAAAGGTTATCGCATTCCTTCGTCAGAAGATGACAGACATGGGATTCCTT
GAAATCCAGACACCTATTCTTTGTGCTTCATCACCAGAGGGTGCAAGAGATTATATCGTACCTTCAAGAAAATATAAGGG
CAAATTCTATGCTCTTCCACAGGCACCACAGCAGTATAAGCAGTTACTTATGGTATCTGGTTTTGATAAATATTTCCAGA
TTGCACCTTGCTTCAGAGATGAGGATGCAAGAGCTGACCGTTCACCAGGAGAATTCTACCAGTTAGATTTCGAGATGAGC
TTTGCTACACAGGAAGATGTATTCAGAATCGGTGAAGAGGTTCTCACAGAAACATTTGCAAAATTCGCACCAGAAGGATA
CGAAGTTACACAGGCACCTTATCCAATCATCAGCTACAAGCAGGCAATGCTTGAGTTTGGTTCAGATAAGCCAGATCTTC
GTAATCCACTTAGAATTATTGACCTTTCAGAATTCTTTAACAAATGTACATTCAAGCCTTTCCAGAACAAGACTGTAAGA
GCAATCAATGTACATGCAAAGCTTTCAAAGGGACAGCATGAGAAGTTACTTAAGTTCGCTACAGGAATCGGAATGGGCGG
ACTTGGATACCTTGAGGTTCTTGAAGACGGAAGCTACAAGGGACCAATTGATAAATTTATTCCAGAAGAACTCAAGGGAG
AAATCAAAGACCTTGCAGGACTTCAGCCAGGAGATACAATCTTCTTCATCGCTGATAAGGAAGACAGAGCTGCGTACTTC
GCAGGACAAATCAGAAACGAACTTGGTGAGAGACTTGACCTCATTGAGAAAAATGCATACAGATTCTGTTATGTTAATGA
CTTCCCAATGTTTGAAAGAGATCCAGAAACTAAGAAGATTGGATTTACACATAATCCATTCTCAATGCCACAGGGTGGAC
TTGAGGCACTTAATACAAAAGATCCACTTGATATTCTTGCATACCAGTATGATATCGTATGCAACGGTGTAGAGTTATCA
TCAGGAGCAGTCCGTAACCATGACATGCAGATAATGGTTAAGGCATTCGAAATTGCCGGATATGACGAAGAAGTATTAAA
GGCAAAATTTGGAGCATTATACAATGCATTCCAGTTTGGTGCACCACCACATGCAGGTATGGCACCAGGTGTTGACCGTA
TGATTATGCTCCTTAGAAATGAAGAGAATATCCGTGAGATAATTCCATTCCCAATGAGCGGAACAGCACAGGATTTAATG
TGCGGAGCACCAAATGAGGTAACAGAGCAGCAGCTTAGGGAAGTTCATATTAAGGTTAGGGATTAG

Upstream 100 bases:

>100_bases
GGCAGATAACTGTCTGGCAAAGAAACAGCGCGGGATGTTAGATTTTATGATTTGACAGGACACAACATTTTTACCTGTCC
AGAAAAGAAAGGAGATTGCA

Downstream 100 bases:

>100_bases
GAGACATACGGACGGCTGCAGGATTTTTATAAATTCACCGCAGGCACGCTCTCGCTGCTTGGAGACTCGCAGCGGTACTA
AACTACTTGGAATTTGGCTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 581; Mature: 580

Protein sequence:

>581_residues
MANMYRDVNLSEVNESYIGKELKVAGWVENIRDHGGVSFIDLRDMYGVLQVVLRDTTLLDGIHKEECIAIEGIMEQRDEE
TYNPKIPSGTIELEAKKITILGQVYQQLPFEVQTSKEVREDVRLKYRYLDLRNQKVKDNMIFRSKVIAFLRQKMTDMGFL
EIQTPILCASSPEGARDYIVPSRKYKGKFYALPQAPQQYKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMS
FATQEDVFRIGEEVLTETFAKFAPEGYEVTQAPYPIISYKQAMLEFGSDKPDLRNPLRIIDLSEFFNKCTFKPFQNKTVR
AINVHAKLSKGQHEKLLKFATGIGMGGLGYLEVLEDGSYKGPIDKFIPEELKGEIKDLAGLQPGDTIFFIADKEDRAAYF
AGQIRNELGERLDLIEKNAYRFCYVNDFPMFERDPETKKIGFTHNPFSMPQGGLEALNTKDPLDILAYQYDIVCNGVELS
SGAVRNHDMQIMVKAFEIAGYDEEVLKAKFGALYNAFQFGAPPHAGMAPGVDRMIMLLRNEENIREIIPFPMSGTAQDLM
CGAPNEVTEQQLREVHIKVRD

Sequences:

>Translated_581_residues
MANMYRDVNLSEVNESYIGKELKVAGWVENIRDHGGVSFIDLRDMYGVLQVVLRDTTLLDGIHKEECIAIEGIMEQRDEE
TYNPKIPSGTIELEAKKITILGQVYQQLPFEVQTSKEVREDVRLKYRYLDLRNQKVKDNMIFRSKVIAFLRQKMTDMGFL
EIQTPILCASSPEGARDYIVPSRKYKGKFYALPQAPQQYKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMS
FATQEDVFRIGEEVLTETFAKFAPEGYEVTQAPYPIISYKQAMLEFGSDKPDLRNPLRIIDLSEFFNKCTFKPFQNKTVR
AINVHAKLSKGQHEKLLKFATGIGMGGLGYLEVLEDGSYKGPIDKFIPEELKGEIKDLAGLQPGDTIFFIADKEDRAAYF
AGQIRNELGERLDLIEKNAYRFCYVNDFPMFERDPETKKIGFTHNPFSMPQGGLEALNTKDPLDILAYQYDIVCNGVELS
SGAVRNHDMQIMVKAFEIAGYDEEVLKAKFGALYNAFQFGAPPHAGMAPGVDRMIMLLRNEENIREIIPFPMSGTAQDLM
CGAPNEVTEQQLREVHIKVRD
>Mature_580_residues
ANMYRDVNLSEVNESYIGKELKVAGWVENIRDHGGVSFIDLRDMYGVLQVVLRDTTLLDGIHKEECIAIEGIMEQRDEET
YNPKIPSGTIELEAKKITILGQVYQQLPFEVQTSKEVREDVRLKYRYLDLRNQKVKDNMIFRSKVIAFLRQKMTDMGFLE
IQTPILCASSPEGARDYIVPSRKYKGKFYALPQAPQQYKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMSF
ATQEDVFRIGEEVLTETFAKFAPEGYEVTQAPYPIISYKQAMLEFGSDKPDLRNPLRIIDLSEFFNKCTFKPFQNKTVRA
INVHAKLSKGQHEKLLKFATGIGMGGLGYLEVLEDGSYKGPIDKFIPEELKGEIKDLAGLQPGDTIFFIADKEDRAAYFA
GQIRNELGERLDLIEKNAYRFCYVNDFPMFERDPETKKIGFTHNPFSMPQGGLEALNTKDPLDILAYQYDIVCNGVELSS
GAVRNHDMQIMVKAFEIAGYDEEVLKAKFGALYNAFQFGAPPHAGMAPGVDRMIMLLRNEENIREIIPFPMSGTAQDLMC
GAPNEVTEQQLREVHIKVRD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=599, Percent_Identity=32.220367278798, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI45439306, Length=259, Percent_Identity=28.1853281853282, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI5031815, Length=259, Percent_Identity=31.2741312741313, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI194272210, Length=259, Percent_Identity=31.2741312741313, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI226491502, Length=251, Percent_Identity=24.7011952191235, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1788173, Length=583, Percent_Identity=39.1080617495712, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1790571, Length=277, Percent_Identity=26.3537906137184, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1789256, Length=270, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=587, Percent_Identity=30.8347529812606, Blast_Score=251, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI71994340, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17535925, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17535927, Length=254, Percent_Identity=27.5590551181102, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17551876, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325153, Length=628, Percent_Identity=31.5286624203822, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6323011, Length=306, Percent_Identity=27.7777777777778, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=297, Percent_Identity=22.2222222222222, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=34.1666666666667, Blast_Score=316, Evalue=4e-86,
Organism=Drosophila melanogaster, GI17136276, Length=249, Percent_Identity=31.7269076305221, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640849, Length=248, Percent_Identity=26.2096774193548, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640851, Length=248, Percent_Identity=26.2096774193548, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19922416, Length=331, Percent_Identity=25.0755287009063, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 66309; Mature: 66178

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANMYRDVNLSEVNESYIGKELKVAGWVENIRDHGGVSFIDLRDMYGVLQVVLRDTTLLD
CCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIHKEECIAIEGIMEQRDEETYNPKIPSGTIELEAKKITILGQVYQQLPFEVQTSKEVRE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHCCCEECCHHHHHH
DVRLKYRYLDLRNQKVKDNMIFRSKVIAFLRQKMTDMGFLEIQTPILCASSPEGARDYIV
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCC
PSRKYKGKFYALPQAPQQYKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMS
CCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEEEE
FATQEDVFRIGEEVLTETFAKFAPEGYEVTQAPYPIISYKQAMLEFGSDKPDLRNPLRII
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEE
DLSEFFNKCTFKPFQNKTVRAINVHAKLSKGQHEKLLKFATGIGMGGLGYLEVLEDGSYK
EHHHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
GPIDKFIPEELKGEIKDLAGLQPGDTIFFIADKEDRAAYFAGQIRNELGERLDLIEKNAY
CCHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCE
RFCYVNDFPMFERDPETKKIGFTHNPFSMPQGGLEALNTKDPLDILAYQYDIVCNGVELS
EEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCHHHCCCCCCHHHHEEEEEEEECCEEEC
SGAVRNHDMQIMVKAFEIAGYDEEVLKAKFGALYNAFQFGAPPHAGMAPGVDRMIMLLRN
CCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
EENIREIIPFPMSGTAQDLMCGAPNEVTEQQLREVHIKVRD
CCCHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHEEEECC
>Mature Secondary Structure 
ANMYRDVNLSEVNESYIGKELKVAGWVENIRDHGGVSFIDLRDMYGVLQVVLRDTTLLD
CCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHC
GIHKEECIAIEGIMEQRDEETYNPKIPSGTIELEAKKITILGQVYQQLPFEVQTSKEVRE
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEHHHHHHHHHCCCEECCHHHHHH
DVRLKYRYLDLRNQKVKDNMIFRSKVIAFLRQKMTDMGFLEIQTPILCASSPEGARDYIV
HHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCCCCCCCCCC
PSRKYKGKFYALPQAPQQYKQLLMVSGFDKYFQIAPCFRDEDARADRSPGEFYQLDFEMS
CCCCCCCEEEECCCCHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEEEE
FATQEDVFRIGEEVLTETFAKFAPEGYEVTQAPYPIISYKQAMLEFGSDKPDLRNPLRII
CCCHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHCCCCCCCCCCCEEEE
DLSEFFNKCTFKPFQNKTVRAINVHAKLSKGQHEKLLKFATGIGMGGLGYLEVLEDGSYK
EHHHHHHHCCCCCCCCCCEEEEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCC
GPIDKFIPEELKGEIKDLAGLQPGDTIFFIADKEDRAAYFAGQIRNELGERLDLIEKNAY
CCHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCE
RFCYVNDFPMFERDPETKKIGFTHNPFSMPQGGLEALNTKDPLDILAYQYDIVCNGVELS
EEEEEECCCCCCCCCCCCEECCCCCCCCCCCCCHHHCCCCCCHHHHEEEEEEEECCEEEC
SGAVRNHDMQIMVKAFEIAGYDEEVLKAKFGALYNAFQFGAPPHAGMAPGVDRMIMLLRN
CCCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
EENIREIIPFPMSGTAQDLMCGAPNEVTEQQLREVHIKVRD
CCCHHHHCCCCCCCCHHHHHCCCCCHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA