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The map label for this gene is ispD

Identifier: 238916347

GI number: 238916347

Start: 410990

End: 411673

Strand: Direct

Name: ispD

Synonym: EUBELI_00381

Alternate gene names: 238916347

Gene position: 410990-411673 (Clockwise)

Preceding gene: 238916346

Following gene: 238916348

Centisome position: 19.17

GC content: 38.01

Gene sequence:

>684_bases
ATGAATACGGCTATAGTTCTTGCGGGTGGAAGTGGAAAAAGAATGCATAGTGAAATTCCAAAGCAGTATATGCAGCTTAA
TGGAAAGCCTGTTATATATTATTCGCTGAAAGCATTTGAAGAAAGTGAACATGTGGATGAGATTATTCTTGTTACAGCAA
AGGAATATATAGAATATGTCAGAAATGAAATTGCAGGTTCTCAATTTGCTAAATTAACAAAAATCATTGAGGGCGGCAAA
GAACGTTTTGATTCAGTGTGGGCAGGTTTACAGCAGATTAGTGAAAAAGGGTATGTATATATACATGATGGGGCAAGACC
ATGTATAAATCAGAATATAATAAATGCGTGCTGGGAAACGGTCGTCCAGACTGATGCGTGTGTTGCGGCAGCACCGGTTA
AAGACACAATAAAAGTTGCAGATGCTGACGAGTATGCAATAAATACACCGGACAGAAAAACATTATGGCAGATTCAGACG
CCACAGGTCTTTTCAGCAGATGTAATTAAAGAAGCATACAGCAGGCTCTATGAACAGAATTGCTTTGATGGGGTTACAGA
TGATGCGATGGTTGTGGAAAGATATGGCAACTCAAAAATAAAACTGGTTAATTGTGGTTACTGTAATATTAAAATAACCA
CGCCAGAAGATATGGAGATTGCGGGCATATTTTTAAAAGGTTGA

Upstream 100 bases:

>100_bases
CTATGATAGGGGCAGCAGCATATTATGAATATATTAAGGGGGTACGTCATGGCTATGATTTAAATGCTGTGCCGAACCTT
AAATTAGGAGAACGCATATA

Downstream 100 bases:

>100_bases
CGAGTTATCAAAATGATAATATAATTAATAAAAATTTTATAAAATAAGGAGAGAGGTATGAAAAACTTTTCAGTAAAAAA
CATTAATTTTGTTGGTGCTG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MNTAIVLAGGSGKRMHSEIPKQYMQLNGKPVIYYSLKAFEESEHVDEIILVTAKEYIEYVRNEIAGSQFAKLTKIIEGGK
ERFDSVWAGLQQISEKGYVYIHDGARPCINQNIINACWETVVQTDACVAAAPVKDTIKVADADEYAINTPDRKTLWQIQT
PQVFSADVIKEAYSRLYEQNCFDGVTDDAMVVERYGNSKIKLVNCGYCNIKITTPEDMEIAGIFLKG

Sequences:

>Translated_227_residues
MNTAIVLAGGSGKRMHSEIPKQYMQLNGKPVIYYSLKAFEESEHVDEIILVTAKEYIEYVRNEIAGSQFAKLTKIIEGGK
ERFDSVWAGLQQISEKGYVYIHDGARPCINQNIINACWETVVQTDACVAAAPVKDTIKVADADEYAINTPDRKTLWQIQT
PQVFSADVIKEAYSRLYEQNCFDGVTDDAMVVERYGNSKIKLVNCGYCNIKITTPEDMEIAGIFLKG
>Mature_227_residues
MNTAIVLAGGSGKRMHSEIPKQYMQLNGKPVIYYSLKAFEESEHVDEIILVTAKEYIEYVRNEIAGSQFAKLTKIIEGGK
ERFDSVWAGLQQISEKGYVYIHDGARPCINQNIINACWETVVQTDACVAAAPVKDTIKVADADEYAINTPDRKTLWQIQT
PQVFSADVIKEAYSRLYEQNCFDGVTDDAMVVERYGNSKIKLVNCGYCNIKITTPEDMEIAGIFLKG

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=232, Percent_Identity=26.7241379310345, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1789104, Length=226, Percent_Identity=32.7433628318584, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_EUBE2 (C4Z312)

Other databases:

- EMBL:   CP001104
- RefSeq:   YP_002929864.1
- GeneID:   7958149
- GenomeReviews:   CP001104_GR
- KEGG:   eel:EUBELI_00381
- OMA:   PSNIKVT
- HAMAP:   MF_00108
- InterPro:   IPR001228
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25421; Mature: 25421

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAIVLAGGSGKRMHSEIPKQYMQLNGKPVIYYSLKAFEESEHVDEIILVTAKEYIEYV
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCEEEEEHHHHHHHH
RNEIAGSQFAKLTKIIEGGKERFDSVWAGLQQISEKGYVYIHDGARPCINQNIINACWET
HHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
VVQTDACVAAAPVKDTIKVADADEYAINTPDRKTLWQIQTPQVFSADVIKEAYSRLYEQN
HHHCCCCEEECCCCHHEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CFDGVTDDAMVVERYGNSKIKLVNCGYCNIKITTPEDMEIAGIFLKG
CCCCCCCCHHHHHHCCCCEEEEEECCEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MNTAIVLAGGSGKRMHSEIPKQYMQLNGKPVIYYSLKAFEESEHVDEIILVTAKEYIEYV
CCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCCCCCCCEEEEEHHHHHHHH
RNEIAGSQFAKLTKIIEGGKERFDSVWAGLQQISEKGYVYIHDGARPCINQNIINACWET
HHHHCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHH
VVQTDACVAAAPVKDTIKVADADEYAINTPDRKTLWQIQTPQVFSADVIKEAYSRLYEQN
HHHCCCCEEECCCCHHEEEECCCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
CFDGVTDDAMVVERYGNSKIKLVNCGYCNIKITTPEDMEIAGIFLKG
CCCCCCCCHHHHHHCCCCEEEEEECCEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA