BacMap

Definition	Eubacterium eligens ATCC 27750 chromosome, complete genome.
Accession	NC_012778
Length	2,144,190

Click here to switch to the map view.

The map label for this gene is yafB [C]

Identifier: 238916155

GI number: 238916155

Start: 216863

End: 217273

Strand: Direct

Name: yafB [C]

Synonym: EUBELI_00189

Alternate gene names: 238916155

Gene position: 216863-217273 (Clockwise)

Preceding gene: 238916154

Following gene: 238916156

Centisome position: 10.11

GC content: 45.5

Gene sequence:

>411_bases
ATGAGTGTTTTGAGCAAAAATTATACATTGAACAACGGTGTGGAGATACCAAAGCTTGGACTTGGAACATGGTTTATTGA
TGATGCGGACGCGGCAGATGCAGTTAGGGCTGCTGTTGCAACGGGCTACCGTCATATTGATACAGCGCAGGCTTACGGTA
ATGAGCGCGGTGTCGGTGAAGGTGTCAGAACCTGTGGTGTGCCGAGAGAGCAGCTTTTTGTCGTATCGAAGGTCGCTGCG
GAGCATAAGACCAATGAGTCGGCAGCCAAGAGTATTGATGAGACTTTGGAAAAAATGGGTCTGGATTATCTGGTCATGAT
GATCATTCATTCGCCACAGCCCCTTTTCACAACAAAAAAAGGTCAATTCACAACATTCCAGATAGATATTGCAAGAACTT
ATCCGATATAA

Upstream 100 bases:

>100_bases
ATAACAGATTAGGATAATGTCTTATATGGCAGGAATGGAGGATTACAATGGCTAAGGAAGTATGGAAAGAAGATTAATTG
AAAGGTTTAGGTAAGAACTG

Downstream 100 bases:

>100_bases
TAGCTATAAATATATGTAAGCAAGGAAGTGACAATATGCAAATAGCTATCTGTGATGATGAAGTATCTATGGTTCAGATA
CTGGAGAAAAAGATAAAAAA

Product: hypothetical protein

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 136; Mature: 135

Protein sequence:

>136_residues
MSVLSKNYTLNNGVEIPKLGLGTWFIDDADAADAVRAAVATGYRHIDTAQAYGNERGVGEGVRTCGVPREQLFVVSKVAA
EHKTNESAAKSIDETLEKMGLDYLVMMIIHSPQPLFTTKKGQFTTFQIDIARTYPI

Sequences:

>Translated_136_residues
MSVLSKNYTLNNGVEIPKLGLGTWFIDDADAADAVRAAVATGYRHIDTAQAYGNERGVGEGVRTCGVPREQLFVVSKVAA
EHKTNESAAKSIDETLEKMGLDYLVMMIIHSPQPLFTTKKGQFTTFQIDIARTYPI
>Mature_135_residues
SVLSKNYTLNNGVEIPKLGLGTWFIDDADAADAVRAAVATGYRHIDTAQAYGNERGVGEGVRTCGVPREQLFVVSKVAAE
HKTNESAAKSIDETLEKMGLDYLVMMIIHSPQPLFTTKKGQFTTFQIDIARTYPI

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=108, Percent_Identity=37.962962962963, Blast_Score=81, Evalue=2e-16,
Organism=Homo sapiens, GI5174391, Length=108, Percent_Identity=37.962962962963, Blast_Score=81, Evalue=2e-16,
Organism=Homo sapiens, GI223468663, Length=108, Percent_Identity=37.962962962963, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI4502049, Length=79, Percent_Identity=45.5696202531646, Blast_Score=72, Evalue=2e-13,
Organism=Homo sapiens, GI24497583, Length=113, Percent_Identity=34.5132743362832, Blast_Score=71, Evalue=3e-13,
Organism=Homo sapiens, GI310109922, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=5e-13,
Organism=Homo sapiens, GI310109926, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=6e-13,
Organism=Homo sapiens, GI93277124, Length=103, Percent_Identity=34.9514563106796, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI310109920, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=7e-13,
Organism=Homo sapiens, GI207028673, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=8e-13,
Organism=Homo sapiens, GI45446745, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=8e-13,
Organism=Homo sapiens, GI4503285, Length=110, Percent_Identity=33.6363636363636, Blast_Score=70, Evalue=8e-13,
Organism=Homo sapiens, GI5453543, Length=110, Percent_Identity=33.6363636363636, Blast_Score=69, Evalue=8e-13,
Organism=Homo sapiens, GI300116271, Length=120, Percent_Identity=35.8333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Homo sapiens, GI300116273, Length=120, Percent_Identity=35.8333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Homo sapiens, GI5174695, Length=120, Percent_Identity=35.8333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Homo sapiens, GI24497585, Length=141, Percent_Identity=28.3687943262411, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1786400, Length=99, Percent_Identity=39.3939393939394, Blast_Score=85, Evalue=2e-18,
Organism=Escherichia coli, GI87082198, Length=106, Percent_Identity=38.6792452830189, Blast_Score=82, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17564128, Length=119, Percent_Identity=37.8151260504202, Blast_Score=83, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17537079, Length=108, Percent_Identity=38.8888888888889, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17537077, Length=114, Percent_Identity=40.3508771929825, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17537075, Length=114, Percent_Identity=40.3508771929825, Blast_Score=79, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17550248, Length=116, Percent_Identity=36.2068965517241, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17561298, Length=110, Percent_Identity=34.5454545454545, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17566692, Length=109, Percent_Identity=33.0275229357798, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538386, Length=117, Percent_Identity=37.6068376068376, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71998625, Length=105, Percent_Identity=40, Blast_Score=72, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17552492, Length=112, Percent_Identity=33.0357142857143, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17561300, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562292, Length=112, Percent_Identity=32.1428571428571, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324694, Length=109, Percent_Identity=44.0366972477064, Blast_Score=100, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320576, Length=115, Percent_Identity=40, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321896, Length=152, Percent_Identity=37.5, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319625, Length=111, Percent_Identity=40.5405405405405, Blast_Score=81, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322556, Length=117, Percent_Identity=35.8974358974359, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320079, Length=124, Percent_Identity=33.0645161290323, Blast_Score=65, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24644950, Length=131, Percent_Identity=36.6412213740458, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI221378297, Length=135, Percent_Identity=35.5555555555556, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20129731, Length=116, Percent_Identity=37.9310344827586, Blast_Score=82, Evalue=8e-17,
Organism=Drosophila melanogaster, GI45553081, Length=143, Percent_Identity=33.5664335664336, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24662789, Length=112, Percent_Identity=34.8214285714286, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24663317, Length=113, Percent_Identity=38.9380530973451, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24657054, Length=106, Percent_Identity=38.6792452830189, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24662781, Length=110, Percent_Identity=39.0909090909091, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24662785, Length=105, Percent_Identity=38.0952380952381, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 14894; Mature: 14763

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVLSKNYTLNNGVEIPKLGLGTWFIDDADAADAVRAAVATGYRHIDTAQAYGNERGVGE
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
GVRTCGVPREQLFVVSKVAAEHKTNESAAKSIDETLEKMGLDYLVMMIIHSPQPLFTTKK
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECC
GQFTTFQIDIARTYPI
CCEEEEEEEEEEECCC
>Mature Secondary Structure 
SVLSKNYTLNNGVEIPKLGLGTWFIDDADAADAVRAAVATGYRHIDTAQAYGNERGVGE
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
GVRTCGVPREQLFVVSKVAAEHKTNESAAKSIDETLEKMGLDYLVMMIIHSPQPLFTTKK
HHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEECC
GQFTTFQIDIARTYPI
CCEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA