Click here to switch to the map view.

The map label for this gene is cca [H]

Identifier: 238916042

GI number: 238916042

Start: 81001

End: 82254

Strand: Reverse

Name: cca [H]

Synonym: EUBELI_00075

Alternate gene names: 238916042

Gene position: 82254-81001 (Counterclockwise)

Preceding gene: 238916051

Following gene: 238916039

Centisome position: 3.84

GC content: 41.47

Gene sequence:

>1254_bases
ATGCCTGAAAACTTTCATATTGACATGCCGGAAGCTGTCAGGAACATTATACATACAATTGAAGAACACGGCTTCAAAGC
CTATGCTGTTGGCGGCTGTATACGAGACAGTATCCTTGGTCGAAACCCGGATGACTGGGATATAACCACTTCTGCTGTTC
CATCACAGATAAAAACTTTCTTTAAAAGAACTGTTGATACTGGAATCGCCCACGGAACCGTCACTATAATGATAGATAAA
ACCGGATACGAAGTTACAACATACAGAATTGATGGCGAATACGAGGATGGCAGGCATCCGAAATCTGTAGAATTTTCAGA
CAGGCTTGTTGATGACCTGTGCCGCCGTGATTTTACGATAAATGCCATGGCATACAACGACACTGATGGATTTGTTGATG
AATTTAACGGTCTTGATGATTTGGATGCAAAAATAATACGCTGCGTTGGTGAACCAGAATTAAGATTTACAGAGGACGCT
CTGCGAATGATGCGTGCCATAAGATTTTCTGCACAGCTTGGCTTTACCATTGATGAAGCAACATGGAATGCAATTAAAAA
GCTTGCCCCATCAATCAGCAAAATAAGCATGGAGCGTGTGCATGTAGAGCTTGGCAAAACACTTATGTCTGCACATCCCG
ATTATGTTGCTCAATATTCAGAAGCAGGACTTTTTGAGCCGATTCTTCCGGTAATTGACAATTCCCTAAAGAGCCGCTAT
AAGCGCAAAATACTTGCAACATTACAACACACACCTGATAATATATACTTACGCTATGCTGCACTTTTCATAGCTTCTAC
ACCTGACGAAGCAGCTAAGACACTTCGAGCACTTAAACTGGATAACAAGACTGTTGACACAGTATCTAAGCTTGTTAAAT
TATCAAAGATTGATATAGAAGAAACTGAGCCGGCCGTTCGTACTGCCCTTAATCAGTATGGCAGAGATTTCCTGCCTCTG
TGGCATGAGCTTATGATGGCTGTAATCCAGGCATCTGAAGACATCACAGGAATAAGTAATCCGGCGAAGGTAAAGCATCT
TCTCACATTAAAGCGTCTTGGGACAGATATTCTTGCACGCGGTGACTGTTTTACAATAAAAGCCCTCGACATATCAGGAA
ATGACCTTATTGAATATGGCCTTCAGGGACACGAAATCGGTGAAACACTGAAATCCCTGCTTGATATCGTTATAGAGAAC
CCTAAGCTCAATGATAAAGCTACTCTTATTGCAATGATTGAACATATCAAATAA

Upstream 100 bases:

>100_bases
CTACAATCATTATCTTGATGAAAATGCATTTTTCTCTTATAATAGACAAATATGGAATCTGAATCCACATATAAATCAAA
CAATACTGCGGGGGAAAATT

Downstream 100 bases:

>100_bases
TATAATCATAAAATAACCCTCACCGGCTGCCTTCTGGCAAATCCGATGAGGGTTATTATAATTAACACTTCTTAGAATTA
ATATGCCTTACCCCAGTAAA

Product: tRNA nucleotidyltransferase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 417; Mature: 416

Protein sequence:

>417_residues
MPENFHIDMPEAVRNIIHTIEEHGFKAYAVGGCIRDSILGRNPDDWDITTSAVPSQIKTFFKRTVDTGIAHGTVTIMIDK
TGYEVTTYRIDGEYEDGRHPKSVEFSDRLVDDLCRRDFTINAMAYNDTDGFVDEFNGLDDLDAKIIRCVGEPELRFTEDA
LRMMRAIRFSAQLGFTIDEATWNAIKKLAPSISKISMERVHVELGKTLMSAHPDYVAQYSEAGLFEPILPVIDNSLKSRY
KRKILATLQHTPDNIYLRYAALFIASTPDEAAKTLRALKLDNKTVDTVSKLVKLSKIDIEETEPAVRTALNQYGRDFLPL
WHELMMAVIQASEDITGISNPAKVKHLLTLKRLGTDILARGDCFTIKALDISGNDLIEYGLQGHEIGETLKSLLDIVIEN
PKLNDKATLIAMIEHIK

Sequences:

>Translated_417_residues
MPENFHIDMPEAVRNIIHTIEEHGFKAYAVGGCIRDSILGRNPDDWDITTSAVPSQIKTFFKRTVDTGIAHGTVTIMIDK
TGYEVTTYRIDGEYEDGRHPKSVEFSDRLVDDLCRRDFTINAMAYNDTDGFVDEFNGLDDLDAKIIRCVGEPELRFTEDA
LRMMRAIRFSAQLGFTIDEATWNAIKKLAPSISKISMERVHVELGKTLMSAHPDYVAQYSEAGLFEPILPVIDNSLKSRY
KRKILATLQHTPDNIYLRYAALFIASTPDEAAKTLRALKLDNKTVDTVSKLVKLSKIDIEETEPAVRTALNQYGRDFLPL
WHELMMAVIQASEDITGISNPAKVKHLLTLKRLGTDILARGDCFTIKALDISGNDLIEYGLQGHEIGETLKSLLDIVIEN
PKLNDKATLIAMIEHIK
>Mature_416_residues
PENFHIDMPEAVRNIIHTIEEHGFKAYAVGGCIRDSILGRNPDDWDITTSAVPSQIKTFFKRTVDTGIAHGTVTIMIDKT
GYEVTTYRIDGEYEDGRHPKSVEFSDRLVDDLCRRDFTINAMAYNDTDGFVDEFNGLDDLDAKIIRCVGEPELRFTEDAL
RMMRAIRFSAQLGFTIDEATWNAIKKLAPSISKISMERVHVELGKTLMSAHPDYVAQYSEAGLFEPILPVIDNSLKSRYK
RKILATLQHTPDNIYLRYAALFIASTPDEAAKTLRALKLDNKTVDTVSKLVKLSKIDIEETEPAVRTALNQYGRDFLPLW
HELMMAVIQASEDITGISNPAKVKHLLTLKRLGTDILARGDCFTIKALDISGNDLIEYGLQGHEIGETLKSLLDIVIENP
KLNDKATLIAMIEHIK

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=223, Percent_Identity=34.9775784753363, Blast_Score=115, Evalue=6e-26,
Organism=Escherichia coli, GI87081691, Length=239, Percent_Identity=32.2175732217573, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789436, Length=221, Percent_Identity=29.4117647058824, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=203, Percent_Identity=31.5270935960591, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321016, Length=227, Percent_Identity=29.0748898678414, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI281360112, Length=214, Percent_Identity=36.9158878504673, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI21357337, Length=214, Percent_Identity=36.9158878504673, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46886; Mature: 46755

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPENFHIDMPEAVRNIIHTIEEHGFKAYAVGGCIRDSILGRNPDDWDITTSAVPSQIKTF
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FKRTVDTGIAHGTVTIMIDKTGYEVTTYRIDGEYEDGRHPKSVEFSDRLVDDLCRRDFTI
HHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
NAMAYNDTDGFVDEFNGLDDLDAKIIRCVGEPELRFTEDALRMMRAIRFSAQLGFTIDEA
EEEEECCCCCHHHHCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCCEECHH
TWNAIKKLAPSISKISMERVHVELGKTLMSAHPDYVAQYSEAGLFEPILPVIDNSLKSRY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KRKILATLQHTPDNIYLRYAALFIASTPDEAAKTLRALKLDNKTVDTVSKLVKLSKIDIE
HHHHHHHHHCCCCHHEEEEEEHHEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
ETEPAVRTALNQYGRDFLPLWHELMMAVIQASEDITGISNPAKVKHLLTLKRLGTDILAR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCC
GDCFTIKALDISGNDLIEYGLQGHEIGETLKSLLDIVIENPKLNDKATLIAMIEHIK
CCEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PENFHIDMPEAVRNIIHTIEEHGFKAYAVGGCIRDSILGRNPDDWDITTSAVPSQIKTF
CCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FKRTVDTGIAHGTVTIMIDKTGYEVTTYRIDGEYEDGRHPKSVEFSDRLVDDLCRRDFTI
HHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEE
NAMAYNDTDGFVDEFNGLDDLDAKIIRCVGEPELRFTEDALRMMRAIRFSAQLGFTIDEA
EEEEECCCCCHHHHCCCCCHHHHHHHHHCCCCCCEECHHHHHHHHHHHHHHHCCCEECHH
TWNAIKKLAPSISKISMERVHVELGKTLMSAHPDYVAQYSEAGLFEPILPVIDNSLKSRY
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
KRKILATLQHTPDNIYLRYAALFIASTPDEAAKTLRALKLDNKTVDTVSKLVKLSKIDIE
HHHHHHHHHCCCCHHEEEEEEHHEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
ETEPAVRTALNQYGRDFLPLWHELMMAVIQASEDITGISNPAKVKHLLTLKRLGTDILAR
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHCC
GDCFTIKALDISGNDLIEYGLQGHEIGETLKSLLDIVIENPKLNDKATLIAMIEHIK
CCEEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA