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The map label for this gene is holB [H]

Identifier: 238915993

GI number: 238915993

Start: 24900

End: 25895

Strand: Direct

Name: holB [H]

Synonym: EUBELI_00026

Alternate gene names: 238915993

Gene position: 24900-25895 (Clockwise)

Preceding gene: 238915992

Following gene: 238915994

Centisome position: 1.16

GC content: 39.06

Gene sequence:

>996_bases
ATGGGTAATTTTTCAGACATATATGGATATGAGACTATTAAAGAACATTTGCAGAGTGCAATATCTATGGGAAAGGTATC
TCATGCTTATGTAATCAGCGGAGGACTTGGTTCAGGAAAGAAGCTTATTGCCAGCACATTTGCACAGACACTGCAGTGTG
AGGCGGGCGGTGTTGAGCCTTGCGGGGTGTGCCATTCCTGTGTGCAGGCAGCAGGCAGGAACCAGCCGGATATTATCTGG
GTTGGACATGAGAAGCCGGGAAGTATTGGTGTTGATGATATAAGAGACCAGCTTGTATCGGATATGCAGATTAAGCCTTA
CAGCAGTCCTTACAAGATATACATTATTGATGAAGCAGACAAGCTTACTGTTGCAGCACAGAATGCAATGCTTAAGACAA
TTGAAGAGCCGCCGGCTTATGGAATTGTTATTCTGCTTGCTAATAATCCGGATGTTTTCTTACAGACTATTCTGTCAAGA
TGTGTTGTGCTTGATCTTAAGCCACTAAAGGATGATATAGTTATTAAATATCTTAAAGACCACTATGATAATATCAGTGA
TTACGAGTGTAAATTTGCAGCAAGATTTGCAGCTGGACGAATCGGCAGGGCAATGACTATGGTTGAATCTACTGAGTTTG
CAGAGTTAAGACGTGATGTAATGGATGTTATTAAGAATGCTAAGGATATGGATTCGACTGATATTATGACATCGGTGAAA
AGAGTTACCAATTATAAGCTGACTATTGATGATTATCTTGACCTTATGCTTATGTGGTACAGGGATGTACTTATGTTTAA
GAGTACGAATGATACTAATCTGCTCATATTTAATGACCAGATGACACTGATAAAGAGTCAGGCACAGACTATGTCATATG
AAGGTTTACAGGATATTATTAATTCTATTGACAGGGTTAAGGTGAGACTTCAGGCAAATGTTAATTTTGATCTGGTTATT
GAATTGCTTATTATGGCGATTAAGGAAAATTCATAG

Upstream 100 bases:

>100_bases
CGGAGCTATTGATGATATCAGGGGATTGTTATTAGATATTTCAACATAGGAGCATTATGTGTTATAATATTACAGATAAG
TAACGAAAAGAGGAATAAGT

Downstream 100 bases:

>100_bases
AATGGAGAGTATAGATGACTAAAGTTGTTGGAGTTAGATTCAGACAGGTTGGAAAGATATATTTTTTTGCACCTGGGAAA
TATTCAGTCGAAACAGGACA

Product: DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MGNFSDIYGYETIKEHLQSAISMGKVSHAYVISGGLGSGKKLIASTFAQTLQCEAGGVEPCGVCHSCVQAAGRNQPDIIW
VGHEKPGSIGVDDIRDQLVSDMQIKPYSSPYKIYIIDEADKLTVAAQNAMLKTIEEPPAYGIVILLANNPDVFLQTILSR
CVVLDLKPLKDDIVIKYLKDHYDNISDYECKFAARFAAGRIGRAMTMVESTEFAELRRDVMDVIKNAKDMDSTDIMTSVK
RVTNYKLTIDDYLDLMLMWYRDVLMFKSTNDTNLLIFNDQMTLIKSQAQTMSYEGLQDIINSIDRVKVRLQANVNFDLVI
ELLIMAIKENS

Sequences:

>Translated_331_residues
MGNFSDIYGYETIKEHLQSAISMGKVSHAYVISGGLGSGKKLIASTFAQTLQCEAGGVEPCGVCHSCVQAAGRNQPDIIW
VGHEKPGSIGVDDIRDQLVSDMQIKPYSSPYKIYIIDEADKLTVAAQNAMLKTIEEPPAYGIVILLANNPDVFLQTILSR
CVVLDLKPLKDDIVIKYLKDHYDNISDYECKFAARFAAGRIGRAMTMVESTEFAELRRDVMDVIKNAKDMDSTDIMTSVK
RVTNYKLTIDDYLDLMLMWYRDVLMFKSTNDTNLLIFNDQMTLIKSQAQTMSYEGLQDIINSIDRVKVRLQANVNFDLVI
ELLIMAIKENS
>Mature_330_residues
GNFSDIYGYETIKEHLQSAISMGKVSHAYVISGGLGSGKKLIASTFAQTLQCEAGGVEPCGVCHSCVQAAGRNQPDIIWV
GHEKPGSIGVDDIRDQLVSDMQIKPYSSPYKIYIIDEADKLTVAAQNAMLKTIEEPPAYGIVILLANNPDVFLQTILSRC
VVLDLKPLKDDIVIKYLKDHYDNISDYECKFAARFAAGRIGRAMTMVESTEFAELRRDVMDVIKNAKDMDSTDIMTSVKR
VTNYKLTIDDYLDLMLMWYRDVLMFKSTNDTNLLIFNDQMTLIKSQAQTMSYEGLQDIINSIDRVKVRLQANVNFDLVIE
LLIMAIKENS

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG2812

COG function: function code L; DNA polymerase III, gamma/tau subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=216, Percent_Identity=32.4074074074074, Blast_Score=114, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37097; Mature: 36966

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNFSDIYGYETIKEHLQSAISMGKVSHAYVISGGLGSGKKLIASTFAQTLQCEAGGVEP
CCCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
CGVCHSCVQAAGRNQPDIIWVGHEKPGSIGVDDIRDQLVSDMQIKPYSSPYKIYIIDEAD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
KLTVAAQNAMLKTIEEPPAYGIVILLANNPDVFLQTILSRCVVLDLKPLKDDIVIKYLKD
CEEHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHH
HYDNISDYECKFAARFAAGRIGRAMTMVESTEFAELRRDVMDVIKNAKDMDSTDIMTSVK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RVTNYKLTIDDYLDLMLMWYRDVLMFKSTNDTNLLIFNDQMTLIKSQAQTMSYEGLQDII
HHHCCEEEHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
NSIDRVKVRLQANVNFDLVIELLIMAIKENS
HHHHHEEEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GNFSDIYGYETIKEHLQSAISMGKVSHAYVISGGLGSGKKLIASTFAQTLQCEAGGVEP
CCCHHHCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCH
CGVCHSCVQAAGRNQPDIIWVGHEKPGSIGVDDIRDQLVSDMQIKPYSSPYKIYIIDEAD
HHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
KLTVAAQNAMLKTIEEPPAYGIVILLANNPDVFLQTILSRCVVLDLKPLKDDIVIKYLKD
CEEHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECCCCCHHHHHHHHHH
HYDNISDYECKFAARFAAGRIGRAMTMVESTEFAELRRDVMDVIKNAKDMDSTDIMTSVK
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RVTNYKLTIDDYLDLMLMWYRDVLMFKSTNDTNLLIFNDQMTLIKSQAQTMSYEGLQDII
HHHCCEEEHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
NSIDRVKVRLQANVNFDLVIELLIMAIKENS
HHHHHEEEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]