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The map label for this gene is lacA [C]

Identifier: 238915988

GI number: 238915988

Start: 14537

End: 15142

Strand: Direct

Name: lacA [C]

Synonym: EUBELI_00012

Alternate gene names: 238915988

Gene position: 14537-15142 (Clockwise)

Preceding gene: 238915987

Following gene: 238915989

Centisome position: 0.68

GC content: 36.96

Gene sequence:

>606_bases
ATGTGGAATATGACAGAGAGACAGAAAATGATTAGTGGACAATTATACAAGTCAGGAGTAAAGGAACTTGTTAATGACAG
AATGTTATGTAAAGAGCAGTGCTATGACTTTAATGCATTAAGACCATCGCAGACGGAAGAACAGACTGCAATGATTAAGA
AGATATTCGGTAAGGTCGGAAGCAACTGTTTTATAACGGCTCCGTTCTGGTGTGATTATGGATATAATATAGAAGCAGGT
GATAATTTCTATGCTAATCATAATCTTATAATACTCGACTGTGCGAAAGTAACATTTGGTAACAATGTATTCATAGCACC
GGACTGTGGTTTCTATACAGCAGGTCACCCTGTTGATGCAGCAAGCCGTAATGAAGGATGGGAATATGCTTATCCTATTA
ATGTAGGTAATGACGTATGGATAGGCGGTGGGGTAAAAGTAATGCCCGGAGTAACAATAGGTAATAATGTTGTAATAGGT
GGTGGAAGCGTAGTTGTAAAAGATATACCAGACAACAGTATTGCAGTAGGTAATCCATGTAAGGTAATAAAAAAGATAGA
ATCAGGCAAAGCAAGGGAATATAATATAGAAGAAAAGCATATATAA

Upstream 100 bases:

>100_bases
CTCATCCCGAGCGGCGAATGACTATCATGAACAAAGTTCATGATATAATGAGCATTAGCGAAGACAAGTTATTCACAATA
TTTGTAAAGTTATAAAGGAA

Downstream 100 bases:

>100_bases
CTTGTATATAAATATAGTGGAAACCCCTATATATAGTGTGATTAAAATAAATTACAACTTTTTTAAAAAAAATTGAAAAA
AGTTGTTGACAATGTGTGTA

Product: galactoside O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MWNMTERQKMISGQLYKSGVKELVNDRMLCKEQCYDFNALRPSQTEEQTAMIKKIFGKVGSNCFITAPFWCDYGYNIEAG
DNFYANHNLIILDCAKVTFGNNVFIAPDCGFYTAGHPVDAASRNEGWEYAYPINVGNDVWIGGGVKVMPGVTIGNNVVIG
GGSVVVKDIPDNSIAVGNPCKVIKKIESGKAREYNIEEKHI

Sequences:

>Translated_201_residues
MWNMTERQKMISGQLYKSGVKELVNDRMLCKEQCYDFNALRPSQTEEQTAMIKKIFGKVGSNCFITAPFWCDYGYNIEAG
DNFYANHNLIILDCAKVTFGNNVFIAPDCGFYTAGHPVDAASRNEGWEYAYPINVGNDVWIGGGVKVMPGVTIGNNVVIG
GGSVVVKDIPDNSIAVGNPCKVIKKIESGKAREYNIEEKHI
>Mature_201_residues
MWNMTERQKMISGQLYKSGVKELVNDRMLCKEQCYDFNALRPSQTEEQTAMIKKIFGKVGSNCFITAPFWCDYGYNIEAG
DNFYANHNLIILDCAKVTFGNNVFIAPDCGFYTAGHPVDAASRNEGWEYAYPINVGNDVWIGGGVKVMPGVTIGNNVVIG
GGSVVVKDIPDNSIAVGNPCKVIKKIESGKAREYNIEEKHI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=192, Percent_Identity=43.75, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI1786664, Length=182, Percent_Identity=42.3076923076923, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1788367, Length=148, Percent_Identity=30.4054054054054, Blast_Score=66, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322243, Length=193, Percent_Identity=48.7046632124352, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22232; Mature: 22232

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWNMTERQKMISGQLYKSGVKELVNDRMLCKEQCYDFNALRPSQTEEQTAMIKKIFGKVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
SNCFITAPFWCDYGYNIEAGDNFYANHNLIILDCAKVTFGNNVFIAPDCGFYTAGHPVDA
CCEEEECCEEECCCCEECCCCCEEECCCEEEEEEEEEEECCEEEECCCCCCCCCCCCCCC
ASRNEGWEYAYPINVGNDVWIGGGVKVMPGVTIGNNVVIGGGSVVVKDIPDNSIAVGNPC
CCCCCCCEEEEEEECCCEEEECCCEEEECCEEECCEEEEECCEEEEEECCCCCEEECCHH
KVIKKIESGKAREYNIEEKHI
HHHHHHHCCCCEECCCHHCCC
>Mature Secondary Structure
MWNMTERQKMISGQLYKSGVKELVNDRMLCKEQCYDFNALRPSQTEEQTAMIKKIFGKVG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHC
SNCFITAPFWCDYGYNIEAGDNFYANHNLIILDCAKVTFGNNVFIAPDCGFYTAGHPVDA
CCEEEECCEEECCCCEECCCCCEEECCCEEEEEEEEEEECCEEEECCCCCCCCCCCCCCC
ASRNEGWEYAYPINVGNDVWIGGGVKVMPGVTIGNNVVIGGGSVVVKDIPDNSIAVGNPC
CCCCCCCEEEEEEECCCEEEECCCEEEECCEEECCEEEEECCEEEEEECCCCCEEECCHH
KVIKKIESGKAREYNIEEKHI
HHHHHHHCCCCEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]