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The map label for this gene is aglB

Identifier: 238897192

GI number: 238897192

Start: 5189919

End: 5191241

Strand: Reverse

Name: aglB

Synonym: KP1_5462

Alternate gene names: 238897192

Gene position: 5191241-5189919 (Counterclockwise)

Preceding gene: 238897193

Following gene: 238897191

Centisome position: 98.91

GC content: 57.52

Gene sequence:

>1323_bases
ATGAAAAAATTCTCAGTTGTTATCGCAGGCGGCGGCAGCACCTTCACCCCGGGCATTGTCCTGATGCTGCTGGCAAACCA
GGATCGTTTTCCGCTGCGGTCGCTGAAATTTTATGACAACGACGGCGCGCGCCAGGAGACCATCGCCGAGGCGTGCAAAG
TGATCCTCAAAGAGCAGGCGCCGGAGATTGAGTTCAGCTATACCACCGATCCGCAGGCGGCGTTTACCGATGTTGATTTT
GTCATGGCGCATATCCGCGTCGGGAAATATCCGATGCGTGAACAAGATGAGAAAATCCCGCTACGCCACGGCGTGCTGGG
CCAGGAGACCTGCGGACCGGGTGGGATCGCCTACGGTATGCGCTCTATCGGCGGCGTGCTGGAGCTGGTGGATTATATGG
AAAAATATTCGCCGAACGCCTGGATGCTTAACTACTCCAACCCGGCGGCGATTGTGGCGGAAGCCACTCGTCGTCTGCGG
CCGAACGCCAAAATCCTCAACATCTGCGATATGCCGATCGGCATTGAAGGGCGGATGGCGCAGATTGTCGGCCTCAAAGA
CCGTAAACAGATGCGCGTGCGCTACTACGGCCTCAACCACTTCGGCTGGTGGACGTCGATTGAGGACCTCGACGGCAATG
ATTTGATGCCGAAACTGCGCGAATATGTGGCGAAATACGGCTATGTGCCGCCCTCGAACGATCCGCACACCGAGGCCAGC
TGGAACGACACCTTTGCCAAAGCGAAAGATGTGCAGGCGCTGGATCCGCAGACCATGCCGAATACCTACCTGAAATATTA
TCTGTTCCCGGACTATGTGGTAGCCCACTCCAACCCGGAACGCACCCGCGCCAATGAGGTGATGGATCATCGGGAGAAAA
ACGTCTTCAGCGCCTGCCGGGCGATCATCGCCGCCGGGAAATCCACCGCCGGCGATCTGGAGATTGACGAGCATGCCTCC
TATATCGTCGATCTGGCGACGGCTATCGCCTTTAACACGCAGGAAAGGATGCTGCTGATTGTGCCGAACAATGGCGCGAT
CCATAACTTTGACGCCGACGCGATGGTGGAGATCCCTTGTCTGGTAGGCCACAACGGACCGGAGCCGCTGACGGTCGGCG
ACATCCCGCATTTCCAGAAAGGGCTGATGAGCCAGCAGGTGGCAGTGGAAAAACTGGTGGTCGATGCCTGGGAACAGCGC
TCGTACCACAAGCTGTGGCAGGCGATTACCCTGTCGAAAACCGTGCCGAGCGCCTCGGTGGCGAAAGCGATCCTCGACGA
CCTCATTGCCGCCAACAAAGATTATTGGCCTGAGCTGCATTAA

Upstream 100 bases:

>100_bases
TCATCGTCGGCCTGCACGTTCCCCAGGTGCGCGACCAGCTGGAAAACCTGATGAAAGATTCTCTTTCGACCGAACATACC
ACCATGACGGAGGCAGTATC

Downstream 100 bases:

>100_bases
TCCCCGTTGTACTACCGGCGCCCTGCGGGGCGCCGGTTCTTCTCCCCGGTTCCTGATTTACGCTATGCTGAAGCCTGCAG
CGCGATGAACAAGGAACTGT

Product: maltose-6'-phosphate glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDF
VMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEAS
WNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHAS
YIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQR
SYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH

Sequences:

>Translated_440_residues
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDF
VMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEAS
WNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHAS
YIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQR
SYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH
>Mature_440_residues
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQAPEIEFSYTTDPQAAFTDVDF
VMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLR
PNAKILNICDMPIGIEGRMAQIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEAS
WNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACRAIIAAGKSTAGDLEIDEHAS
YIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPCLVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQR
SYHKLWQAITLSKTVPSASVAKAILDDLIAANKDYWPELH

Specific function: Is involved in the catabolism of alpha-glycosides accumulated via a phosphoenolpyruvate-dependent phosphotransferase system (PEP-PTS). Hydrolyzes a wide variety of 6-phospho-alpha-D- glucosides including maltose-6'-phosphate, isomaltose-6'- phosphate, mal

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family

Homologues:

Organism=Escherichia coli, GI1788029, Length=449, Percent_Identity=27.3942093541203, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1790560, Length=461, Percent_Identity=24.0780911062907, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AGLB_KLEPN (Q9AGA6)

Other databases:

- EMBL:   AF337811
- ProteinModelPortal:   Q9AGA6
- SMR:   Q9AGA6
- BRENDA:   3.2.1.122
- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00732

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =3.2.1.122

Molecular weight: Translated: 49256; Mature: 49256

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: ACT_SITE 170-170 ACT_SITE 263-263 BINDING 93-93 BINDING 147-147 BINDING 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQA
CCEEEEEEECCCCCCCHHHEEEEEECCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHCC
PEIEFSYTTDPQAAFTDVDFVMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGM
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
RSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGRMA
HHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCE
QIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEAS
EEECCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
WNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACR
CCHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPC
HHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCEEEEEE
LVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQRSYHKLWQAITLSKTVPSASV
EECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AKAILDDLIAANKDYWPELH
HHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKFSVVIAGGGSTFTPGIVLMLLANQDRFPLRSLKFYDNDGARQETIAEACKVILKEQA
CCEEEEEEECCCCCCCHHHEEEEEECCCCCCCHHEEEECCCCCHHHHHHHHHHHHHHHCC
PEIEFSYTTDPQAAFTDVDFVMAHIRVGKYPMREQDEKIPLRHGVLGQETCGPGGIAYGM
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHCCCCCCCCCCCCHHHHHH
RSIGGVLELVDYMEKYSPNAWMLNYSNPAAIVAEATRRLRPNAKILNICDMPIGIEGRMA
HHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCE
QIVGLKDRKQMRVRYYGLNHFGWWTSIEDLDGNDLMPKLREYVAKYGYVPPSNDPHTEAS
EEECCCCCCCEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
WNDTFAKAKDVQALDPQTMPNTYLKYYLFPDYVVAHSNPERTRANEVMDHREKNVFSACR
CCHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
AIIAAGKSTAGDLEIDEHASYIVDLATAIAFNTQERMLLIVPNNGAIHNFDADAMVEIPC
HHHHCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCEEEEEE
LVGHNGPEPLTVGDIPHFQKGLMSQQVAVEKLVVDAWEQRSYHKLWQAITLSKTVPSASV
EECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AKAILDDLIAANKDYWPELH
HHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11473129; 11322729