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The map label for this gene is ilvB [H]

Identifier: 238897180

GI number: 238897180

Start: 5179187

End: 5180875

Strand: Reverse

Name: ilvB [H]

Synonym: KP1_5449

Alternate gene names: 238897180

Gene position: 5180875-5179187 (Counterclockwise)

Preceding gene: 238897181

Following gene: 238897179

Centisome position: 98.71

GC content: 61.81

Gene sequence:

>1689_bases
ATGGCAAGTTCGGGCACCACATCAAACACAATGCGCTTTACCGGCGCGCAGCTGGTTGTTCATTTACTGGAGCGCCAGGG
TATCACTATGGTCAGCGGCATTCCGGGCGGCTCCATCCTGCCTATCTACGATGCCTTAAGCCAGAGCACGCAGATCCGCC
ACATCCTGGCGCGCCACGAGCAGGGCGCGGGCTTTATCGCCCAGGGGATGGCGCGTACCGAAGGCAAGCCGGCGGTCTGT
ATGGCCTGCAGCGGCCCGGGGGCGACGAACCTGGTGACGGCGATCGCCGATGCCCGCCTGGATTCGATTCCGCTGGTCTG
CATCACCGGCCAGGTACCGGCCTCGATGATCGGTACCGACGCCTTCCAGGAAGTGGACACCTACGGCATCTCTATCCCCA
TCACCAAACATAACTATCTGGTGCGCGACATTGCCGAGCTGCCGCAGGTGATCAGCGACGCCTTCCGTATTGCCCAGTCC
GGGCGTCCCGGGCCGGTGTGGATAGACATTCCTAAGGACGTACAGTCGGCGACCATTGAGCTGGAAGCGCTGCCTGAGCC
TGGCGAGCGCGCCCCGGCACCGGCATTCGCGCCTGAAAGCGTGCGTGAAGCGGCGGCAATGATCAACGCGGCGAAACGCC
CGGTACTGTATCTGGGCGGCGGGGTGATTAACGCGCCGCAGGCGATCCGTGAGCTGGCGGAAAAAGCCAACCTGCCGACC
ACCATGACCTTAATGGCGCTGGGCATGCTGCCGAAGGCGCACCCGCTGTCGCTGGGGATGCTGGGGATGCACGGCGCGCG
CAGCACCAACTTCATTCTGCAGGAGGCGGATTTACTGATTGTTTTAGGCGCCCGTTTTGATGACCGGGCGATTGGCAAGA
CCGAGCAGTTCTGCCCGAACGCGAAGATTATCCACGTCGATATCGACCGCGCCGAACTGGGCAAAATTAAGCAACCGCAC
GTGGCGATTCAGGGCGATGTGGCGGAAGTCTTAGCCCAGCTGAACCCGCAGATTGAGGCGCAGCCGCGCGAGGAATGGCG
CCAGCTGGTGGCCGACTTGCAGCGCGAATTCCCCTGCGCCATCCCGCAGGAAAGCGACCCGCTCTCCCATTACGGCCTGA
TTAACGCCGTGGCCGCCTGCGTGGATGATGAAGCGATCATCACTACCGACGTTGGCCAGCACCAGATGTGGACGGCGCAG
GCCTACCCGCTCAATCGTCCGCGCCAGTGGCTGACCTCCGGCGGCCTCGGCACCATGGGCTTCGGCCTGCCGGCGGCCAT
CGGTGCGGCGCTGGCGAATCCGCAGCGCAAGGTTATCTGCTTCTCCGGCGACGGCAGCCTGATGATGAATATTCAGGAGA
TGGCCACCGCCGCCGAAAATCAGCTGGATGTCAAAATTATTCTGCTGAACAACGAAGCGCTGGGGCTGGTGCATCAGCAG
CAGAGCCTGTTCTATCAGCAGGGCGTTTTTGCCGCCACCTATCCGGGGATGATTAACTTTATGCAGATTGCCGCCGGTTT
TGGCCTGCAAACCTGTGATTTAAATAATGAAGTCGATCCGCAGGCGGCGCTGCAGGCGATTATCGACCGTCCGGGGCCGG
CGCTGATCCACGTGCGCATCGACGCACAGCAGAAAGTGTATCCGATGGTACCGCCGGGTGCGGCCAATACTGAAATGGTG
GGGGAATAA

Upstream 100 bases:

>100_bases
CGGAACAACACGAATTCCAAACCCCGCCGGCGCAAACCGAGCGGGGTTTTTTGTTGGCGAGCCCCCCGGTAAAGTCGGCC
CAGAAGAAAAGGACTGGAGC

Downstream 100 bases:

>100_bases
GCCATGCAAAAACAATGTGATAACGTCATTCTGGAACTCACCGTCCGCAACCATCCCGGCGTCATGACCCACGTCTGCGG
GCTTTTTGCCCGTCGCGCGT

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: AHAS-I; Acetohydroxy-acid synthase I large subunit; ALS-I [H]

Number of amino acids: Translated: 562; Mature: 561

Protein sequence:

>562_residues
MASSGTTSNTMRFTGAQLVVHLLERQGITMVSGIPGGSILPIYDALSQSTQIRHILARHEQGAGFIAQGMARTEGKPAVC
MACSGPGATNLVTAIADARLDSIPLVCITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRDIAELPQVISDAFRIAQS
GRPGPVWIDIPKDVQSATIELEALPEPGERAPAPAFAPESVREAAAMINAAKRPVLYLGGGVINAPQAIRELAEKANLPT
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQGDVAEVLAQLNPQIEAQPREEWRQLVADLQREFPCAIPQESDPLSHYGLINAVAACVDDEAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILLNNEALGLVHQQ
QSLFYQQGVFAATYPGMINFMQIAAGFGLQTCDLNNEVDPQAALQAIIDRPGPALIHVRIDAQQKVYPMVPPGAANTEMV
GE

Sequences:

>Translated_562_residues
MASSGTTSNTMRFTGAQLVVHLLERQGITMVSGIPGGSILPIYDALSQSTQIRHILARHEQGAGFIAQGMARTEGKPAVC
MACSGPGATNLVTAIADARLDSIPLVCITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRDIAELPQVISDAFRIAQS
GRPGPVWIDIPKDVQSATIELEALPEPGERAPAPAFAPESVREAAAMINAAKRPVLYLGGGVINAPQAIRELAEKANLPT
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPH
VAIQGDVAEVLAQLNPQIEAQPREEWRQLVADLQREFPCAIPQESDPLSHYGLINAVAACVDDEAIITTDVGQHQMWTAQ
AYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILLNNEALGLVHQQ
QSLFYQQGVFAATYPGMINFMQIAAGFGLQTCDLNNEVDPQAALQAIIDRPGPALIHVRIDAQQKVYPMVPPGAANTEMV
GE
>Mature_561_residues
ASSGTTSNTMRFTGAQLVVHLLERQGITMVSGIPGGSILPIYDALSQSTQIRHILARHEQGAGFIAQGMARTEGKPAVCM
ACSGPGATNLVTAIADARLDSIPLVCITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRDIAELPQVISDAFRIAQSG
RPGPVWIDIPKDVQSATIELEALPEPGERAPAPAFAPESVREAAAMINAAKRPVLYLGGGVINAPQAIRELAEKANLPTT
MTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHV
AIQGDVAEVLAQLNPQIEAQPREEWRQLVADLQREFPCAIPQESDPLSHYGLINAVAACVDDEAIITTDVGQHQMWTAQA
YPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILLNNEALGLVHQQQ
SLFYQQGVFAATYPGMINFMQIAAGFGLQTCDLNNEVDPQAALQAIIDRPGPALIHVRIDAQQKVYPMVPPGAANTEMVG
E

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=27.787610619469, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=25.4937163375224, Blast_Score=159, Evalue=6e-39,
Organism=Escherichia coli, GI1790104, Length=562, Percent_Identity=88.6120996441281, Blast_Score=1024, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=563, Percent_Identity=39.7868561278863, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1786717, Length=483, Percent_Identity=35.4037267080745, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1787096, Length=551, Percent_Identity=28.1306715063521, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1788716, Length=485, Percent_Identity=28.0412371134021, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17531299, Length=478, Percent_Identity=27.4058577405858, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17531301, Length=478, Percent_Identity=27.4058577405858, Blast_Score=158, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17542570, Length=529, Percent_Identity=27.0321361058601, Blast_Score=152, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6323755, Length=576, Percent_Identity=41.4930555555556, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=25.6565656565657, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323073, Length=481, Percent_Identity=21.4137214137214, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922626, Length=481, Percent_Identity=29.5218295218295, Blast_Score=183, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 60120; Mature: 59989

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSGTTSNTMRFTGAQLVVHLLERQGITMVSGIPGGSILPIYDALSQSTQIRHILARHE
CCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHCCHHHHHHHHHHCC
QGAGFIAQGMARTEGKPAVCMACSGPGATNLVTAIADARLDSIPLVCITGQVPASMIGTD
CCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRDIAELPQVISDAFRIAQSGRPGPVWIDIPKDVQSATIE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEE
LEALPEPGERAPAPAFAPESVREAAAMINAAKRPVLYLGGGVINAPQAIRELAEKANLPT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCH
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQGDVAEVLAQLNPQIEAQPREEWRQLVADLQREFPCA
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
IPQESDPLSHYGLINAVAACVDDEAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILLNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
QSLFYQQGVFAATYPGMINFMQIAAGFGLQTCDLNNEVDPQAALQAIIDRPGPALIHVRI
HHHHHHCCCEEEECCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAQQKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
ASSGTTSNTMRFTGAQLVVHLLERQGITMVSGIPGGSILPIYDALSQSTQIRHILARHE
CCCCCCCCEEEEHHHHHHHHHHHHCCCEEEECCCCCCEEHHHHHHCCHHHHHHHHHHCC
QGAGFIAQGMARTEGKPAVCMACSGPGATNLVTAIADARLDSIPLVCITGQVPASMIGTD
CCCCHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCH
AFQEVDTYGISIPITKHNYLVRDIAELPQVISDAFRIAQSGRPGPVWIDIPKDVQSATIE
HHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEE
LEALPEPGERAPAPAFAPESVREAAAMINAAKRPVLYLGGGVINAPQAIRELAEKANLPT
EEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCH
TMTLMALGMLPKAHPLSLGMLGMHGARSTNFILQEADLLIVLGARFDDRAIGKTEQFCPN
HHHHHHHHCCCCCCCCCEEEECCCCCCCCCEEEEECCEEEEEECCCCCCCCCCHHHHCCC
AKIIHVDIDRAELGKIKQPHVAIQGDVAEVLAQLNPQIEAQPREEWRQLVADLQREFPCA
CEEEEEECCHHHHCCCCCCCEEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCC
IPQESDPLSHYGLINAVAACVDDEAIITTDVGQHQMWTAQAYPLNRPRQWLTSGGLGTMG
CCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHCCCCCCCC
FGLPAAIGAALANPQRKVICFSGDGSLMMNIQEMATAAENQLDVKIILLNNEALGLVHQQ
CCHHHHHHHHHCCCCCEEEEECCCCCEEEEHHHHHHHHCCCCEEEEEEECCCCEEEHHHH
QSLFYQQGVFAATYPGMINFMQIAAGFGLQTCDLNNEVDPQAALQAIIDRPGPALIHVRI
HHHHHHCCCEEEECCCHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEEE
DAQQKVYPMVPPGAANTEMVGE
CCCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2989782; 2989781; 7686882; 9278503 [H]