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The map label for this gene is purR [H]

Identifier: 238897159

GI number: 238897159

Start: 5159373

End: 5160215

Strand: Reverse

Name: purR [H]

Synonym: KP1_5423

Alternate gene names: 238897159

Gene position: 5160215-5159373 (Counterclockwise)

Preceding gene: 238897164

Following gene: 238897158

Centisome position: 98.32

GC content: 55.52

Gene sequence:

>843_bases
TTGGATTTCGCTCACATTATTCTGTCGATTTGGCGATGTTTTGCGCTGAAATGGAAAATCGGTTTCCAAAGAAATGGCGA
AATGGATATACTGCGCTGGTCACAATTGAAGCGATTCAGGTTTACAGGATTTTGGCGGGACGCGGCGCCCGCGCAGGGGA
AAATGATGTCGACAATCAATGATGTTTCACGACTTGCCGGGGTTTCCAAAGCCACGGTGTCGCGAGTGTTGAGCGGCTCA
CGTGGCGTGAAGGAAGCCAGTCGTCAGGCGGTGCTGCAGGCGGCGGAAGCGCTCAATTACCGGCCAAACATGATAGCCCA
GTCGTTGCTCAGCCAGTCCACCGGCTGCATCGGCGTCATCTGCGCCCAGGACAACATCAACCAAACCACCAGCTATCTCT
ACGCCCTGGAAAAACAGCTCAGCCAGCACCAGAAACACCTGCTGCTGCGCTTCGCCAACACCAGCCACGGCGTGATGAAC
TCTCTGGAGGAGTTGACCTGCGGGCTGTGCGATAACGTGCTAATCATCGGCGCGCGTTTTCCTCTGAATATTAACCGCCC
GGACGTGGTGCTGGTGGACTGCCTCGATAGCGAAGGCGACAACAGTATTCAGTTCGATCATGCTTTCGCCGCCGAAACCG
CCTGTCACTATCTGATTAGCCAGGGGAGACGGCAGATTGCACTGATTCATCCGCAGAGCAGCGGCTTTGCCGACCAGGTG
CTGCTGGGCTACAAGCACGCGCTGGAAAAAACTTTTTGCCGTTTAATCGCAACCTGGTGTTTCTGGATAACACCTCGCCG
TCGGTGGCGGTGCAGGAGCTGGTCAATAACGCCACGACGCTGA

Upstream 100 bases:

>100_bases
TATTGCGTGGGTGATTTGATATCAGACGGAAACCGGTTTCCATTTATAAAAAGCAGAACTGTGATAGTGGTCAAGATCTC
CTGCTTATAGGGCGGATTTA

Downstream 100 bases:

>100_bases
ACTTTAACGCGCTGCTGGTGAGCGATGAACAGCAGGCGCAGCGCGTGGTGCCGCAGCTGCAGGCGTTTAACCGCGCCGTT
CCGCAAAATGTCATGGTGTT

Product: putative LacI family regulatory protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MDFAHIILSIWRCFALKWKIGFQRNGEMDILRWSQLKRFRFTGFWRDAAPAQGKMMSTINDVSRLAGVSKATVSRVLSGS
RGVKEASRQAVLQAAEALNYRPNMIAQSLLSQSTGCIGVICAQDNINQTTSYLYALEKQLSQHQKHLLLRFANTSHGVMN
SLEELTCGLCDNVLIIGARFPLNINRPDVVLVDCLDSEGDNSIQFDHAFAAETACHYLISQGRRQIALIHPQSSGFADQV
LLGYKHALEKTFCRLIATWCFWITPRRRWRCRSWSITPRR

Sequences:

>Translated_280_residues
MDFAHIILSIWRCFALKWKIGFQRNGEMDILRWSQLKRFRFTGFWRDAAPAQGKMMSTINDVSRLAGVSKATVSRVLSGS
RGVKEASRQAVLQAAEALNYRPNMIAQSLLSQSTGCIGVICAQDNINQTTSYLYALEKQLSQHQKHLLLRFANTSHGVMN
SLEELTCGLCDNVLIIGARFPLNINRPDVVLVDCLDSEGDNSIQFDHAFAAETACHYLISQGRRQIALIHPQSSGFADQV
LLGYKHALEKTFCRLIATWCFWITPRRRWRCRSWSITPRR
>Mature_280_residues
MDFAHIILSIWRCFALKWKIGFQRNGEMDILRWSQLKRFRFTGFWRDAAPAQGKMMSTINDVSRLAGVSKATVSRVLSGS
RGVKEASRQAVLQAAEALNYRPNMIAQSLLSQSTGCIGVICAQDNINQTTSYLYALEKQLSQHQKHLLLRFANTSHGVMN
SLEELTCGLCDNVLIIGARFPLNINRPDVVLVDCLDSEGDNSIQFDHAFAAETACHYLISQGRRQIALIHPQSSGFADQV
LLGYKHALEKTFCRLIATWCFWITPRRRWRCRSWSITPRR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=207, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788474, Length=229, Percent_Identity=31.8777292576419, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789068, Length=210, Percent_Identity=31.4285714285714, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787948, Length=215, Percent_Identity=27.906976744186, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790194, Length=209, Percent_Identity=30.1435406698565, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787580, Length=218, Percent_Identity=27.9816513761468, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786540, Length=218, Percent_Identity=28.8990825688073, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 31794; Mature: 31794

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFAHIILSIWRCFALKWKIGFQRNGEMDILRWSQLKRFRFTGFWRDAAPAQGKMMSTIN
CCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCHHHHHHH
DVSRLAGVSKATVSRVLSGSRGVKEASRQAVLQAAEALNYRPNMIAQSLLSQSTGCIGVI
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
CAQDNINQTTSYLYALEKQLSQHQKHLLLRFANTSHGVMNSLEELTCGLCDNVLIIGARF
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PLNINRPDVVLVDCLDSEGDNSIQFDHAFAAETACHYLISQGRRQIALIHPQSSGFADQV
CCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
LLGYKHALEKTFCRLIATWCFWITPRRRWRCRSWSITPRR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCEECCCCCCCCC
>Mature Secondary Structure
MDFAHIILSIWRCFALKWKIGFQRNGEMDILRWSQLKRFRFTGFWRDAAPAQGKMMSTIN
CCHHHHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCEEECCCCCCCCHHHHHHH
DVSRLAGVSKATVSRVLSGSRGVKEASRQAVLQAAEALNYRPNMIAQSLLSQSTGCIGVI
HHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
CAQDNINQTTSYLYALEKQLSQHQKHLLLRFANTSHGVMNSLEELTCGLCDNVLIIGARF
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEEEC
PLNINRPDVVLVDCLDSEGDNSIQFDHAFAAETACHYLISQGRRQIALIHPQSSGFADQV
CCCCCCCCEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHH
LLGYKHALEKTFCRLIATWCFWITPRRRWRCRSWSITPRR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA