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The map label for this gene is emrB [C]

Identifier: 238897140

GI number: 238897140

Start: 5137317

End: 5138810

Strand: Reverse

Name: emrB [C]

Synonym: KP1_5401

Alternate gene names: 238897140

Gene position: 5138810-5137317 (Counterclockwise)

Preceding gene: 238897142

Following gene: 238897138

Centisome position: 97.91

GC content: 62.85

Gene sequence:

>1494_bases
ATGACTTCTCAAGTCGCTAACCCACCGGTGCAGTCGATACGCCTGCTGTTCAGCGCATTGCTGCTGGTGATGCTGCTTTC
AGCGCTGGATCAGACCATTGTCTCCACCGCGCTGCCGACGATTGTCGGCGAACTGGGCGGGCTGGACAAGCTTTCCTGGG
TGGTCACCGCCTATATCCTCAGCTCCACCATCGCGGTGCCGCTGTACGGTAAATTTGGCGATCTGTTCGGCCGCAAAATT
GTGCTGCAGGTGGCGATTGGGCTGTTTCTCGTCGGCTCCGCGCTCTGCGGGCTGGCGCAGAATATGACCCAACTGGTACT
GATGCGCGGCCTGCAGGGGCTCGGCGGCGGCGGCCTGATGGTGATCAGCATGGCGGCGGTCGCCGACGTCATCCCGCCCG
CCAACCGCGGCCGCTATCAGGGGCTGTTCGGCGGCGTTTTCGGCCTGGCGACGGTGATCGGCCCGCTGATCGGCGGTTTT
CTGGTGCAGCACGCCTCCTGGCGCTGGATCTTCTATATCAACCTGCCGCTCGGCCTGTTCGCGCTGCTGGTGATCGGCGC
GGTATTCCACAGCAGCAATAAACGCAGCCAGCATCAGATTGACTGGCTGGGGGCGATTTATCTCAGCATGGCGCTGCTGT
GCATCATCCTGTTTACCTCGGAAGGGGGCAGCGTCCATGCGTGGAATGACCCGCAGCTGTGGTGCATTCTGGCCTTCGGC
ATCGTGGGGATCATCGGCTTTATTTACGAAGAACGGATGGCCGCCGAGCCGATTATTCCGCTGGCGCTGTTCCGCAACCG
CAGCTTCCTGCTGTGCAGCCTGATCGGCTTCGTCATCGGCATGTCGCTGTTCGGGTCAGTCACCTTCCTGCCGCTCTATC
TGCAGGTGGTGAAGGAGGCGACGCCCACCGAGGCCGGGTTACAGTTAATCCCGCTGATGGGAGGCCTGCTGCTGACCTCG
ATTATCAGCGGGCGGATTATCAGCCGTACCGGTAAATATCGCCTGTTTCCGATCCTCGGCACCCTGCTCGGCGTGACCGG
CATGGTACTGCTGACCCGCATCACCATTCACTCGCCGCTGTGGCAGCTGTACCTGTTTACCGGCGTGCTGGGAGCGGGTC
TGGGGCTGGTGATGCAGGTGCTGGTCCTGGCGGTGCAGAATGCGATGCCGGCGCAGATGTACGGCGTCGCCACCTCCGGC
GTGACCCTGTTCCGCTCGATTGGCGGCTCCATCGGCGTGGCGCTGTTTGGCGCGGTGTTCACGCACGTGCTGCAGAGCAA
CCTGCAACAACTGCTGCCGGAAGGCGCGGTGCTGCCGCCGGGAATGAATCCGGTGGCGGTCCAGCACCTGCCCGCGGATA
TCCGCCTCGACTACCTCGACGCCTTCGGCGCGGCGATCCACGCGGCGTTTCTGATGGCGGCCGGCATTATGGCGGTGGCG
TTTGTCCTCTCATGGCTGTTAAAGGAGGCGCCGCTGAAGACCGCGACGCATTAA

Upstream 100 bases:

>100_bases
TGATCCTTTTGTGCTGTCATGATATGGCGATCTCTTGACCCGACTCATTCTTTCACTAAATATTAATTATGCATTTAATT
AATTTCAAGCGAGCGTTGCA

Downstream 100 bases:

>100_bases
GCGGCCCCGGCGATGCGCAGCAGTGCCTGACGCAGCGCCGGTGCGGCAAAATCGATAAATTTGCGCAGCTTAAGCGGCGC
CAGATCCCGAGCGGTATACA

Product: probably multidrug resistance membrane translocase

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MTSQVANPPVQSIRLLFSALLLVMLLSALDQTIVSTALPTIVGELGGLDKLSWVVTAYILSSTIAVPLYGKFGDLFGRKI
VLQVAIGLFLVGSALCGLAQNMTQLVLMRGLQGLGGGGLMVISMAAVADVIPPANRGRYQGLFGGVFGLATVIGPLIGGF
LVQHASWRWIFYINLPLGLFALLVIGAVFHSSNKRSQHQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFG
IVGIIGFIYEERMAAEPIIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEATPTEAGLQLIPLMGGLLLTS
IISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPLWQLYLFTGVLGAGLGLVMQVLVLAVQNAMPAQMYGVATSG
VTLFRSIGGSIGVALFGAVFTHVLQSNLQQLLPEGAVLPPGMNPVAVQHLPADIRLDYLDAFGAAIHAAFLMAAGIMAVA
FVLSWLLKEAPLKTATH

Sequences:

>Translated_497_residues
MTSQVANPPVQSIRLLFSALLLVMLLSALDQTIVSTALPTIVGELGGLDKLSWVVTAYILSSTIAVPLYGKFGDLFGRKI
VLQVAIGLFLVGSALCGLAQNMTQLVLMRGLQGLGGGGLMVISMAAVADVIPPANRGRYQGLFGGVFGLATVIGPLIGGF
LVQHASWRWIFYINLPLGLFALLVIGAVFHSSNKRSQHQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFG
IVGIIGFIYEERMAAEPIIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEATPTEAGLQLIPLMGGLLLTS
IISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPLWQLYLFTGVLGAGLGLVMQVLVLAVQNAMPAQMYGVATSG
VTLFRSIGGSIGVALFGAVFTHVLQSNLQQLLPEGAVLPPGMNPVAVQHLPADIRLDYLDAFGAAIHAAFLMAAGIMAVA
FVLSWLLKEAPLKTATH
>Mature_496_residues
TSQVANPPVQSIRLLFSALLLVMLLSALDQTIVSTALPTIVGELGGLDKLSWVVTAYILSSTIAVPLYGKFGDLFGRKIV
LQVAIGLFLVGSALCGLAQNMTQLVLMRGLQGLGGGGLMVISMAAVADVIPPANRGRYQGLFGGVFGLATVIGPLIGGFL
VQHASWRWIFYINLPLGLFALLVIGAVFHSSNKRSQHQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFGI
VGIIGFIYEERMAAEPIIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEATPTEAGLQLIPLMGGLLLTSI
ISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPLWQLYLFTGVLGAGLGLVMQVLVLAVQNAMPAQMYGVATSGV
TLFRSIGGSIGVALFGAVFTHVLQSNLQQLLPEGAVLPPGMNPVAVQHLPADIRLDYLDAFGAAIHAAFLMAAGIMAVAF
VLSWLLKEAPLKTATH

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=419, Percent_Identity=26.0143198090692, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081983, Length=418, Percent_Identity=28.7081339712919, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790195, Length=372, Percent_Identity=29.8387096774194, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=25.0614250614251, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788392, Length=337, Percent_Identity=30.2670623145401, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790146, Length=173, Percent_Identity=28.3236994219653, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=478, Percent_Identity=29.2887029288703, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6322958, Length=477, Percent_Identity=25.7861635220126, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323735, Length=535, Percent_Identity=27.2897196261682, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6325455, Length=507, Percent_Identity=23.6686390532544, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=374, Percent_Identity=21.6577540106952, Blast_Score=100, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6319783, Length=390, Percent_Identity=23.5897435897436, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324954, Length=391, Percent_Identity=23.2736572890026, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52905; Mature: 52774

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQVANPPVQSIRLLFSALLLVMLLSALDQTIVSTALPTIVGELGGLDKLSWVVTAYIL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SSTIAVPLYGKFGDLFGRKIVLQVAIGLFLVGSALCGLAQNMTQLVLMRGLQGLGGGGLM
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VISMAAVADVIPPANRGRYQGLFGGVFGLATVIGPLIGGFLVQHASWRWIFYINLPLGLF
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHH
ALLVIGAVFHSSNKRSQHQIDWLGAIYLSMALLCIILFTSEGGSVHAWNDPQLWCILAFG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
IVGIIGFIYEERMAAEPIIPLALFRNRSFLLCSLIGFVIGMSLFGSVTFLPLYLQVVKEA
HHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TPTEAGLQLIPLMGGLLLTSIISGRIISRTGKYRLFPILGTLLGVTGMVLLTRITIHSPL
CCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
WQLYLFTGVLGAGLGLVMQVLVLAVQNAMPAQMYGVATSGVTLFRSIGGSIGVALFGAVF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
THVLQSNLQQLLPEGAVLPPGMNPVAVQHLPADIRLDYLDAFGAAIHAAFLMAAGIMAVA
HHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVLSWLLKEAPLKTATH
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSQVANPPVQSIRLLFSALLLVMLLSALDQTIVSTALPTIVGELGGLDKLSWVVTAYIL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH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HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]