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The map label for this gene is ada [H]

Identifier: 238897094

GI number: 238897094

Start: 5087143

End: 5088201

Strand: Direct

Name: ada [H]

Synonym: KP1_5347

Alternate gene names: 238897094

Gene position: 5087143-5088201 (Clockwise)

Preceding gene: 238897093

Following gene: 238897096

Centisome position: 96.93

GC content: 54.86

Gene sequence:

>1059_bases
ATGAAAATTACCGACAGCATGCAGTGCGATATCTGGTATCAGGCGCTGCTTGAACGCGCGGTGGAATTCACCGGCGTTTT
TTTCGTCGGCGTGAAAACCACCGGCGTATTTTGTATCTCGGTCTGTCGGGCGCGAAAACCCCGGCGGGAAAACGTCGAAT
TTTATAATGACTTTAAATCCGCGCTGGATGCCGGCTTCCGGCCATGCAAAGTCTGTCGCCCGACGGAGAATGCCCGGACG
GCCCCCGCCTTTGTTGAACAAGCGCTCCGCCTGCTGCGAGAAGCTCCAAAGGTCCGCCTCAGCGATAGCGAGCTACGTCA
GCACGATATTAGCCCGGAACGCGTTCGCCGCTGGTTCCTGCAAAACCACGGGATCACCTTTCAGGCTTTTCAACGGATGC
AGCGTTTGAATATGGCGCTTCAGGAATTAAAAGCAGGCCGCTCAACTACCGATGTCGCTTTCGATAGCGGTTACGAGTCC
TTAAGCGGCTTCGGCTATACCTGCAAAAAATTGACCGGTTTCGCACCCAGCGCGCAACGCCAGGTTGTTTTAATTCATCG
TTTTACCACCCCGTTAGGGCCAATGTTTGTTTGTGCAACGCAACGGGGAATTTGTCTGCTGGAGTTTGTCGACAGACGAG
CGCTGGAGTCAGAATTCAGCGACCTGCAGCGGCGGTTCAACGCCAGCATCATCGCAGGTGAAAATGCCCACACCAGGCAG
GCGCAACAGGAAATAACGGAGTATTTCGCCGGACAGCGTCAATCGTTCGAGGTGGCGCTTGATACCCCAGGCAGCGAATT
CCAGCGAGCGGTCTGGCGCCGTTTACAGCACGTTAGCTTCGGTGAGACGACGCATTATCAATCGATTGCGATGGAGATAG
GTAAACCAACGGCCACGCGCGCGGTGGCTGCTGCAAACGGCGCTAACCGCGTCGCGATTATCATCCCGTGCCATCGAATT
ATCGGCAAAGATGGTTCGATGACCGGTTACGGCGGCGGCATTGCACGAAAAATATGGCTTATTAACCATGAGGCAACCAT
CAGGGAAAAACGCGGCTGA

Upstream 100 bases:

>100_bases
TTTCGATGGGCAATTTTATTTATGCTGCGACGCAGGCCAGACTGAAAGACTTATTATGTCAGGTGCAAACCCAACACTCT
TTCTCAGGTGTTTTTTGACC

Downstream 100 bases:

>100_bases
TTTTACTTTCAGGATCAACGGGCCCCTCCCTGACTTTCACCGCGGCTCGGTAAGCCTATTTTTTATCCAGCGCCCGGCAG
GTTAAAAACGCCCCTGCCGC

Product: putative methylated-DNA-[protein]-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MKITDSMQCDIWYQALLERAVEFTGVFFVGVKTTGVFCISVCRARKPRRENVEFYNDFKSALDAGFRPCKVCRPTENART
APAFVEQALRLLREAPKVRLSDSELRQHDISPERVRRWFLQNHGITFQAFQRMQRLNMALQELKAGRSTTDVAFDSGYES
LSGFGYTCKKLTGFAPSAQRQVVLIHRFTTPLGPMFVCATQRGICLLEFVDRRALESEFSDLQRRFNASIIAGENAHTRQ
AQQEITEYFAGQRQSFEVALDTPGSEFQRAVWRRLQHVSFGETTHYQSIAMEIGKPTATRAVAAANGANRVAIIIPCHRI
IGKDGSMTGYGGGIARKIWLINHEATIREKRG

Sequences:

>Translated_352_residues
MKITDSMQCDIWYQALLERAVEFTGVFFVGVKTTGVFCISVCRARKPRRENVEFYNDFKSALDAGFRPCKVCRPTENART
APAFVEQALRLLREAPKVRLSDSELRQHDISPERVRRWFLQNHGITFQAFQRMQRLNMALQELKAGRSTTDVAFDSGYES
LSGFGYTCKKLTGFAPSAQRQVVLIHRFTTPLGPMFVCATQRGICLLEFVDRRALESEFSDLQRRFNASIIAGENAHTRQ
AQQEITEYFAGQRQSFEVALDTPGSEFQRAVWRRLQHVSFGETTHYQSIAMEIGKPTATRAVAAANGANRVAIIIPCHRI
IGKDGSMTGYGGGIARKIWLINHEATIREKRG
>Mature_352_residues
MKITDSMQCDIWYQALLERAVEFTGVFFVGVKTTGVFCISVCRARKPRRENVEFYNDFKSALDAGFRPCKVCRPTENART
APAFVEQALRLLREAPKVRLSDSELRQHDISPERVRRWFLQNHGITFQAFQRMQRLNMALQELKAGRSTTDVAFDSGYES
LSGFGYTCKKLTGFAPSAQRQVVLIHRFTTPLGPMFVCATQRGICLLEFVDRRALESEFSDLQRRFNASIIAGENAHTRQ
AQQEITEYFAGQRQSFEVALDTPGSEFQRAVWRRLQHVSFGETTHYQSIAMEIGKPTATRAVAAANGANRVAIIIPCHRI
IGKDGSMTGYGGGIARKIWLINHEATIREKRG

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=31.3253012048193, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788542, Length=361, Percent_Identity=34.9030470914127, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1787596, Length=168, Percent_Identity=36.9047619047619, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=45.679012345679, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI17137554, Length=145, Percent_Identity=31.0344827586207, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 39826; Mature: 39826

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITDSMQCDIWYQALLERAVEFTGVFFVGVKTTGVFCISVCRARKPRRENVEFYNDFKS
CCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALDAGFRPCKVCRPTENARTAPAFVEQALRLLREAPKVRLSDSELRQHDISPERVRRWFL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
QNHGITFQAFQRMQRLNMALQELKAGRSTTDVAFDSGYESLSGFGYTCKKLTGFAPSAQR
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCHHHHHCCCCCCCCC
QVVLIHRFTTPLGPMFVCATQRGICLLEFVDRRALESEFSDLQRRFNASIIAGENAHTRQ
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
AQQEITEYFAGQRQSFEVALDTPGSEFQRAVWRRLQHVSFGETTHYQSIAMEIGKPTATR
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHH
AVAAANGANRVAIIIPCHRIIGKDGSMTGYGGGIARKIWLINHEATIREKRG
HHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHCCCC
>Mature Secondary Structure
MKITDSMQCDIWYQALLERAVEFTGVFFVGVKTTGVFCISVCRARKPRRENVEFYNDFKS
CCCCCCCCHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ALDAGFRPCKVCRPTENARTAPAFVEQALRLLREAPKVRLSDSELRQHDISPERVRRWFL
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHH
QNHGITFQAFQRMQRLNMALQELKAGRSTTDVAFDSGYESLSGFGYTCKKLTGFAPSAQR
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHCCCCHHHHHCCCCCCCCC
QVVLIHRFTTPLGPMFVCATQRGICLLEFVDRRALESEFSDLQRRFNASIIAGENAHTRQ
EEEEEEECCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHH
AQQEITEYFAGQRQSFEVALDTPGSEFQRAVWRRLQHVSFGETTHYQSIAMEIGKPTATR
HHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHH
AVAAANGANRVAIIIPCHRIIGKDGSMTGYGGGIARKIWLINHEATIREKRG
HHHHCCCCCEEEEEEECHHHHCCCCCCCCCCCCCEEEEEEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]