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The map label for this gene is bcsC [H]

Identifier: 238896979

GI number: 238896979

Start: 4961831

End: 4962940

Strand: Reverse

Name: bcsC [H]

Synonym: KP1_5222

Alternate gene names: 238896979

Gene position: 4962940-4961831 (Counterclockwise)

Preceding gene: 238896980

Following gene: 238896978

Centisome position: 94.56

GC content: 62.34

Gene sequence:

>1110_bases
ATGATGAAAGTACTTTGTGGCGCGGTGCTCTCGGCGCTGCTGCTGGCGGCGGGCCAGGTGGGCGCCGCCTGCCAGTGGCC
CGCCTGGGAGCAGTTTAAACAAGCGTACGTCAGCCCCGAGGGACGGGTGATTGACCCCAGCGATGCGCGCAAAATCTCCA
CCTCGGAAGGACAGAGCTATGGCCTGTTTTTCGCCCTGGCGGCCAACGATCGCGCAGGGTTCGACAAACTGCTGACCTGG
ACGCAGAACAACCTGGCGGAAGGCGATCTGAAACAACATTTGCCGGGCTGGCTGTGGGGCAAAAAGGACGATGAGCAGTG
GACCCTGCTGGACAGCAACTCGGCGTCCGACTCGGATCTGTGGATAGCCTGGGCGCTGCTGGAGGCGGGGCGCCTGTGGC
AACAGCCGCAGTACACCGAGACCGGTAAAGCCCTGCTGGCGCGGATTGTCGCTGAAGAGACGGTGGCGGTGCCGGGCCTC
GGCACGATGCTGCTGCCGGGAAAAGTCGGTTTTGCCGATGACAGCGGCTGGCGGTTTAACCCCAGCTATCTTCCGCCGCA
GCTGGCCACCTACTTTGTGCGTTTTGGCGCGCCGTGGCCCGCTCTGCGCGATAGTAACCTGCGCCTGCTGCTGGAGACCG
CGCCGAAAGGCTTTACCCCGGACTGGGTGCGCTATGAGAAAGGGAAAGGCTGGCAGCTGAAAACCGAAAAGCCGCCGATC
GGCAGCTATGACGCGATTCGCGTCTACCTGTGGGTGGGCATGCTCCATGATGGCGATAAGCAGAAAGCGCGTCTGTTGCA
ACGCTTCGCGCCGATGGCGGCGCAGACCACGAAGCAGGGGGTACCCCCGGAGAAAGTGAATATCGCCACCGGCAAAACCA
GCGGCCAGGGGCCAGTGGGCTTCTCCGCGGCAATGCTGCCGTTTTTACAGGACGACGAGGCCCGGTCGGTGCAACGCCAG
CGCGTCGCCGATAACTATCCCGGCGCGGATGCCTACTACAGCGCAGTTCTGACGCTGTTCGGCCAGGGTTGGGATCAACA
CCGTTTTCGTTTCACGGCGAGTGGTGAATTACAACCTGACTGGAACCAGGAATGCGCAAGCTCTCACTAA

Upstream 100 bases:

>100_bases
GATCTTCGCCGCCATCAGCATTGTGCTGCTGGCATGGGTGCTGTGGCGCATGCTGCGCATCATCAGCCGCCGCCGTCTTA
GCCTGGATGATGAGTAAACC

Downstream 100 bases:

>100_bases
GTTTACTCACGCTGTCCCTCGGCGTTGCGCTGCTGCCGTTAGCGCAGGCGGCGACGACGCCTGCCCAGGAGCATCTGCTG
GAGCAGGTCCGCCTCGGCGA

Product: endo-1,4-D-glucanase

Products: NA

Alternate protein names: Carboxymethylcellulase; CMCase; Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MMKVLCGAVLSALLLAAGQVGAACQWPAWEQFKQAYVSPEGRVIDPSDARKISTSEGQSYGLFFALAANDRAGFDKLLTW
TQNNLAEGDLKQHLPGWLWGKKDDEQWTLLDSNSASDSDLWIAWALLEAGRLWQQPQYTETGKALLARIVAEETVAVPGL
GTMLLPGKVGFADDSGWRFNPSYLPPQLATYFVRFGAPWPALRDSNLRLLLETAPKGFTPDWVRYEKGKGWQLKTEKPPI
GSYDAIRVYLWVGMLHDGDKQKARLLQRFAPMAAQTTKQGVPPEKVNIATGKTSGQGPVGFSAAMLPFLQDDEARSVQRQ
RVADNYPGADAYYSAVLTLFGQGWDQHRFRFTASGELQPDWNQECASSH

Sequences:

>Translated_369_residues
MMKVLCGAVLSALLLAAGQVGAACQWPAWEQFKQAYVSPEGRVIDPSDARKISTSEGQSYGLFFALAANDRAGFDKLLTW
TQNNLAEGDLKQHLPGWLWGKKDDEQWTLLDSNSASDSDLWIAWALLEAGRLWQQPQYTETGKALLARIVAEETVAVPGL
GTMLLPGKVGFADDSGWRFNPSYLPPQLATYFVRFGAPWPALRDSNLRLLLETAPKGFTPDWVRYEKGKGWQLKTEKPPI
GSYDAIRVYLWVGMLHDGDKQKARLLQRFAPMAAQTTKQGVPPEKVNIATGKTSGQGPVGFSAAMLPFLQDDEARSVQRQ
RVADNYPGADAYYSAVLTLFGQGWDQHRFRFTASGELQPDWNQECASSH
>Mature_369_residues
MMKVLCGAVLSALLLAAGQVGAACQWPAWEQFKQAYVSPEGRVIDPSDARKISTSEGQSYGLFFALAANDRAGFDKLLTW
TQNNLAEGDLKQHLPGWLWGKKDDEQWTLLDSNSASDSDLWIAWALLEAGRLWQQPQYTETGKALLARIVAEETVAVPGL
GTMLLPGKVGFADDSGWRFNPSYLPPQLATYFVRFGAPWPALRDSNLRLLLETAPKGFTPDWVRYEKGKGWQLKTEKPPI
GSYDAIRVYLWVGMLHDGDKQKARLLQRFAPMAAQTTKQGVPPEKVNIATGKTSGQGPVGFSAAMLPFLQDDEARSVQRQ
RVADNYPGADAYYSAVLTLFGQGWDQHRFRFTASGELQPDWNQECASSH

Specific function: Hydrolyzes carboxymethylcellulose [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=368, Percent_Identity=72.554347826087, Blast_Score=572, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 40901; Mature: 40901

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKVLCGAVLSALLLAAGQVGAACQWPAWEQFKQAYVSPEGRVIDPSDARKISTSEGQSY
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCEEECCCCCCC
GLFFALAANDRAGFDKLLTWTQNNLAEGDLKQHLPGWLWGKKDDEQWTLLDSNSASDSDL
EEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHH
WIAWALLEAGRLWQQPQYTETGKALLARIVAEETVAVPGLGTMLLPGKVGFADDSGWRFN
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEC
PSYLPPQLATYFVRFGAPWPALRDSNLRLLLETAPKGFTPDWVRYEKGKGWQLKTEKPPI
CCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEECCCCEEEECCCCCC
GSYDAIRVYLWVGMLHDGDKQKARLLQRFAPMAAQTTKQGVPPEKVNIATGKTSGQGPVG
CCHHHEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
FSAAMLPFLQDDEARSVQRQRVADNYPGADAYYSAVLTLFGQGWDQHRFRFTASGELQPD
HHHHHCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
WNQECASSH
CCHHHCCCC
>Mature Secondary Structure
MMKVLCGAVLSALLLAAGQVGAACQWPAWEQFKQAYVSPEGRVIDPSDARKISTSEGQSY
CHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCCEEECCCCCCC
GLFFALAANDRAGFDKLLTWTQNNLAEGDLKQHLPGWLWGKKDDEQWTLLDSNSASDSDL
EEEEEEECCCCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHH
WIAWALLEAGRLWQQPQYTETGKALLARIVAEETVAVPGLGTMLLPGKVGFADDSGWRFN
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCEEC
PSYLPPQLATYFVRFGAPWPALRDSNLRLLLETAPKGFTPDWVRYEKGKGWQLKTEKPPI
CCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCHHEEECCCCEEEECCCCCC
GSYDAIRVYLWVGMLHDGDKQKARLLQRFAPMAAQTTKQGVPPEKVNIATGKTSGQGPVG
CCHHHEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
FSAAMLPFLQDDEARSVQRQRVADNYPGADAYYSAVLTLFGQGWDQHRFRFTASGELQPD
HHHHHCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
WNQECASSH
CCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]