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The map label for this gene is tusA [H]

Identifier: 238896933

GI number: 238896933

Start: 4907342

End: 4907587

Strand: Reverse

Name: tusA [H]

Synonym: KP1_5173

Alternate gene names: 238896933

Gene position: 4907587-4907342 (Counterclockwise)

Preceding gene: 238896936

Following gene: 238896930

Centisome position: 93.5

GC content: 60.98

Gene sequence:

>246_bases
ATGAGCGAACTTTTCTCCACTGCTGACCACACCCTGGATGCCCTGGGACTCCGCTGCCCGGAGCCGGTCATGATGGTGCG
TAAAACCGTGCGGACGATGCCGGTGGGCGAGACGCTGCTGATTATTGCTGACGATCCGGCCACCACCCGCGATATCCCGG
GATTCTGCCGCTTTATGGAGCATGAGCTGGTGGCCCAGGAAACCGAAGCCCTGCCGTACCGCTACCTGATCCGCAAAAGC
CACTGA

Upstream 100 bases:

>100_bases
CGGCGTAATGCAATGGTTGTTTTTCGCGCAAACGTTAACCTGGCTTGCGTACTGGCGAGCAGGGGGTAAACTAGGGCCGT
TTTATTGACGTGAGACTAAA

Downstream 100 bases:

>100_bases
GCCTCTCCCGGATGGCGGCGCGTAGCGCCTTATCCGGGCTACCCGACGGTCTGGAACCGTAGCCCTGCTAAGCGTAGCGC
GAGCAGGGAAGCCGGTATCG

Product: sulfur transfer protein SirA

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 81; Mature: 80

Protein sequence:

>81_residues
MSELFSTADHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFMEHELVAQETEALPYRYLIRKS
H

Sequences:

>Translated_81_residues
MSELFSTADHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFMEHELVAQETEALPYRYLIRKS
H
>Mature_80_residues
SELFSTADHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFMEHELVAQETEALPYRYLIRKSH

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=80, Percent_Identity=85, Blast_Score=143, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 9234; Mature: 9103

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELFSTADHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFME
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
HELVAQETEALPYRYLIRKSH
HHHHHHHHHCCCHHHHEECCC
>Mature Secondary Structure 
SELFSTADHTLDALGLRCPEPVMMVRKTVRTMPVGETLLIIADDPATTRDIPGFCRFME
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHH
HELVAQETEALPYRYLIRKSH
HHHHHHHHHCCCHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA