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The map label for this gene is rpoH [H]

Identifier: 238896924

GI number: 238896924

Start: 4898398

End: 4899252

Strand: Reverse

Name: rpoH [H]

Synonym: KP1_5163

Alternate gene names: 238896924

Gene position: 4899252-4898398 (Counterclockwise)

Preceding gene: 238896925

Following gene: 238896922

Centisome position: 93.35

GC content: 56.02

Gene sequence:

>855_bases
ATGACCAAAGAAATGCAAACTTTAGCTTTAGCCCCCGTTGGTAACCTGGAATCGTACATCCGGGCCGCTAACACTTGGCC
GATGCTGTCGGCTGATGAAGAGCGGGAGCTTGCTGAAAAGCTGCATTACCAGGGCGATCTGGAAGCAGCGAAAAAGCTGA
TTCTGTCTCACCTGCGCTTTGTTGTTCACATTGCTCGTAACTACTCGGGCTATGGCCTGCCGCAGGCGGATCTGATCCAG
GAAGGCAATATCGGCCTGATGAAAGCCGTGCGTCGCTTCAACCCGGAAGTGGGTGTGCGCCTGGTCTCCTTCGCCGTGCA
CTGGATTAAAGCCGAAATTCACGAATACGTGCTGCGCAACTGGCGTATTGTGAAGGTCGCTACCACCAAAGCACAGCGTA
AGCTGTTCTTTAACCTGCGTAAAACCAAACAGCGTTTGGGTTGGTTCAACCAGGATGAAGTGGAAATGGTGGCCCGTGAG
CTGGGCGTGTCGAGCAAAGATGTCCGCGAGATGGAATCCCGTATGGCGGCCCAGGACATGACCTTCGACATGTCGTCCGA
CGATGAGTCCGACAGCCAGCCGATGGCGCCGGTCCTGTATCTGCAGGACAAAACGTCTAACTTTGCCGACGGCATCGAAG
ACGATAACTGGGAAGAGCAGGCGGCGAATAAGCTGACTGATGCGATGCAGGGCCTGGACGAGCGTAGCCAGGACATCATC
CGCGCCCGCTGGCTGGACGAAGATAACAAGTCCACGCTGCAGGAACTGGCCGACCGCTACGGCGTCTCCGCAGAGCGCGT
GCGTCAGCTGGAAAAGAACGCCATGAAAAAACTGCGTGCCGCTATCGAAGCCTGA

Upstream 100 bases:

>100_bases
TACTAATGTGAGTGATATTCTCGTTGCTCATGAATGCTGGCATGCTTGTTGCCTGAGGGGGACAAGAACGCGTCAGAAGA
TATCAATTGAGAGGATTTGA

Downstream 100 bases:

>100_bases
TACGCCATGCCTGCCTGATACCTGGTATCAGGCTCCCCGATAACGCCCCGCCTCAGGTTGGGGCGTTTTGTTTTTCTGCG
TCCTCCCGGAGCAGGAGCAT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTKEMQTLALAPVGNLESYIRAANTWPMLSADEERELAEKLHYQGDLEAAKKLILSHLRFVVHIARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
LGVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKTSNFADGIEDDNWEEQAANKLTDAMQGLDERSQDII
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA

Sequences:

>Translated_284_residues
MTKEMQTLALAPVGNLESYIRAANTWPMLSADEERELAEKLHYQGDLEAAKKLILSHLRFVVHIARNYSGYGLPQADLIQ
EGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
LGVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKTSNFADGIEDDNWEEQAANKLTDAMQGLDERSQDII
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
>Mature_283_residues
TKEMQTLALAPVGNLESYIRAANTWPMLSADEERELAEKLHYQGDLEAAKKLILSHLRFVVHIARNYSGYGLPQADLIQE
GNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVAREL
GVSSKDVREMESRMAAQDMTFDMSSDDESDSQPMAPVLYLQDKTSNFADGIEDDNWEEQAANKLTDAMQGLDERSQDIIR
ARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=284, Percent_Identity=95.0704225352113, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1789098, Length=268, Percent_Identity=27.2388059701493, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789448, Length=242, Percent_Identity=28.5123966942149, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 32637; Mature: 32506

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKEMQTLALAPVGNLESYIRAANTWPMLSADEERELAEKLHYQGDLEAAKKLILSHLRF
CCCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHIARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVSSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDMSSDDESDSQPMAPVLYLQDKTSNFADGIEDDNWEEQAANKLTDAMQGLDERSQDII
CCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TKEMQTLALAPVGNLESYIRAANTWPMLSADEERELAEKLHYQGDLEAAKKLILSHLRF
CCCHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVHIARNYSGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVSSKDVREMESRMAAQDM
CEEEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
TFDMSSDDESDSQPMAPVLYLQDKTSNFADGIEDDNWEEQAANKLTDAMQGLDERSQDII
CCCCCCCCCCCCCCCCCEEEEECCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA
HHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7501460 [H]