Click here to switch to the map view.

The map label for this gene is puuE [H]

Identifier: 238896922

GI number: 238896922

Start: 4895438

End: 4896703

Strand: Reverse

Name: puuE [H]

Synonym: KP1_5161

Alternate gene names: 238896922

Gene position: 4896703-4895438 (Counterclockwise)

Preceding gene: 238896924

Following gene: 238896921

Centisome position: 93.3

GC content: 62.01

Gene sequence:

>1266_bases
GTGAAAAGCTCAGAACTGAATCAACGTCGCCAGCAGGCGACGCCGCGCGGCGTAGGCGTAATGTGTAACTACTTTGTCGA
GAAGGCGGAAAACGCCACGCTGTGGGATATCGAAGGCAATGAGGTGATCGATTTCGCCGCCGGGATCGCCGTGTTAAATA
CCGGTCACCGCCATCCGAAAGTGGTCGCCGCGGTAGCCGAGCAGCTGCAGGCGTTCACCCACACCGCCTATCAGATTGTG
CCGTACGAAAGCTATGTTTCGCTGGCGGAGCGTATCAACGATCTGGCGCCAATTGACGGGCCGGCAAAAACGGCTTTCTT
CACCACCGGCGCGGAAGCGGTCGAGAACGCGGTGAAAATCGCCCGCGCTTACACCGGTCGTCCGGGGCTGATTACCTTCG
GCGGCGGTTTCCACGGCCGTACCTTTATGACCATGGCCCTCACCGGTAAGGTCGCGCCCTACAAAATCGGCTTCGGGCCG
TTCCCGGGCTCGGTGTATCACGGTGTCTACCCGAATGCGGCGCATGGCGTGACCACCGCGGATGCGCTGAAAAGCCTGGA
GCGCATTTTCAAAGCGGATATTGCGCCGGATCAGGTCGCGGCCATCATCCTCGAGCCGATCCAGGGGGAAGGCGGCTTTA
ACGTCGCGCCGGCCGATTTTATGCAGGCGCTGCGCGACCTGTGCGATACCCACGGCATTCTGCTGATCGCCGATGAAGTG
CAGACCGGTTTCGCCCGCACCGGCAAGCTGTTCGCTATGCAGCACTATGAGGTGAAACCGGATCTGATGACGATGGCGAA
AAGCCTCGCCGGCGGTTTCCCGCTGTCGGGCGTTGTCGGTCGCGCCGAGGTGATGGATGCGCCAGCGCCTGGCGGGCTGG
GCGGAACCTATGCCGGTAACCCGCTGGCGGTGGCCGCGGCGCACGCGGTGCTGGATGTCATTGCCGAAGAGCAGCTGTGC
CAGCGCGCTGAACAGCTGGGTAGCCATCTGCAGGAAGTGCTCAACCAGGCGCGCGCTACCTGCCCGGCGATCGTTGATGT
CCGCGGCCGGGGGTCGATGGTGGCGGTGGAGTTTAACGATCCGCAAACAGGCGAACCGTCGCCGGAATTCACCCGCCAGG
TGCAACAGAAAGCGCAGGAGAACGGCCTGCTGCTGCTGAGCTGTGGCGTCTACGGCAACGTGATCCGCTTCCTGTATCCG
TTGACTATCCCGGATGCCCAGTTCTCGAAAGCGCTGGATATTCTGGCGCGCGTACTGAAGAGCTAA

Upstream 100 bases:

>100_bases
CAAAATTGGTACTCGATTATAGGGCCAACTCCTGTGTAAATAAACGACATCGTTTCTAATTTGTTGCGATAACGTGACAT
ACACGACAGGAGATTCGCAG

Downstream 100 bases:

>100_bases
CGTCTCCTCGGCGGGCTGCGGCGGCAGGGAAGCCGAACAGAAACAGGACGCCGCAGCCGGCTGTTTATGCTCAGCGTGAG
TGGTTTATCACCCTGATATC

Product: 4-aminobutyrate aminotransferase

Products: NA

Alternate protein names: GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MKSSELNQRRQQATPRGVGVMCNYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPKVVAAVAEQLQAFTHTAYQIV
PYESYVSLAERINDLAPIDGPAKTAFFTTGAEAVENAVKIARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGP
FPGSVYHGVYPNAAHGVTTADALKSLERIFKADIAPDQVAAIILEPIQGEGGFNVAPADFMQALRDLCDTHGILLIADEV
QTGFARTGKLFAMQHYEVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGNPLAVAAAHAVLDVIAEEQLC
QRAEQLGSHLQEVLNQARATCPAIVDVRGRGSMVAVEFNDPQTGEPSPEFTRQVQQKAQENGLLLLSCGVYGNVIRFLYP
LTIPDAQFSKALDILARVLKS

Sequences:

>Translated_421_residues
MKSSELNQRRQQATPRGVGVMCNYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPKVVAAVAEQLQAFTHTAYQIV
PYESYVSLAERINDLAPIDGPAKTAFFTTGAEAVENAVKIARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGP
FPGSVYHGVYPNAAHGVTTADALKSLERIFKADIAPDQVAAIILEPIQGEGGFNVAPADFMQALRDLCDTHGILLIADEV
QTGFARTGKLFAMQHYEVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGNPLAVAAAHAVLDVIAEEQLC
QRAEQLGSHLQEVLNQARATCPAIVDVRGRGSMVAVEFNDPQTGEPSPEFTRQVQQKAQENGLLLLSCGVYGNVIRFLYP
LTIPDAQFSKALDILARVLKS
>Mature_421_residues
MKSSELNQRRQQATPRGVGVMCNYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPKVVAAVAEQLQAFTHTAYQIV
PYESYVSLAERINDLAPIDGPAKTAFFTTGAEAVENAVKIARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGP
FPGSVYHGVYPNAAHGVTTADALKSLERIFKADIAPDQVAAIILEPIQGEGGFNVAPADFMQALRDLCDTHGILLIADEV
QTGFARTGKLFAMQHYEVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGNPLAVAAAHAVLDVIAEEQLC
QRAEQLGSHLQEVLNQARATCPAIVDVRGRGSMVAVEFNDPQTGEPSPEFTRQVQQKAQENGLLLLSCGVYGNVIRFLYP
LTIPDAQFSKALDILARVLKS

Specific function: Involved in the breakdown of putrescine via transamination of gamma-aminobutyrate [H]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=411, Percent_Identity=31.6301703163017, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI13994255, Length=419, Percent_Identity=31.0262529832936, Blast_Score=169, Evalue=5e-42,
Organism=Homo sapiens, GI284507298, Length=325, Percent_Identity=32.3076923076923, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI37574042, Length=432, Percent_Identity=29.1666666666667, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI226442709, Length=386, Percent_Identity=30.5699481865285, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI24119277, Length=398, Percent_Identity=28.643216080402, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI226442705, Length=432, Percent_Identity=28.4722222222222, Blast_Score=145, Evalue=5e-35,
Organism=Homo sapiens, GI188536080, Length=445, Percent_Identity=28.0898876404494, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI38679950, Length=445, Percent_Identity=28.0898876404494, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI38679946, Length=445, Percent_Identity=28.0898876404494, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1787560, Length=420, Percent_Identity=73.8095238095238, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=418, Percent_Identity=55.9808612440191, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1788044, Length=395, Percent_Identity=36.7088607594937, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789759, Length=402, Percent_Identity=33.8308457711443, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI145693181, Length=341, Percent_Identity=38.1231671554252, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786349, Length=345, Percent_Identity=33.0434782608696, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1786991, Length=352, Percent_Identity=31.25, Blast_Score=142, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25144271, Length=412, Percent_Identity=33.252427184466, Blast_Score=194, Evalue=7e-50,
Organism=Caenorhabditis elegans, GI32564660, Length=431, Percent_Identity=32.4825986078886, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI71992977, Length=453, Percent_Identity=29.5805739514349, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25144274, Length=229, Percent_Identity=37.117903930131, Blast_Score=141, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17541228, Length=436, Percent_Identity=27.0642201834862, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323470, Length=412, Percent_Identity=34.9514563106796, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6324432, Length=409, Percent_Identity=31.2958435207824, Blast_Score=192, Evalue=9e-50,
Organism=Saccharomyces cerevisiae, GI6324386, Length=330, Percent_Identity=26.969696969697, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321456, Length=387, Percent_Identity=27.390180878553, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI21357415, Length=411, Percent_Identity=33.0900243309002, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI28574759, Length=446, Percent_Identity=32.2869955156951, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI161085790, Length=448, Percent_Identity=31.25, Blast_Score=184, Evalue=7e-47,
Organism=Drosophila melanogaster, GI21356575, Length=429, Percent_Identity=31.002331002331, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI281366494, Length=460, Percent_Identity=26.304347826087, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24667139, Length=460, Percent_Identity=26.304347826087, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24667143, Length=460, Percent_Identity=26.304347826087, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004632
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: =2.6.1.19 [H]

Molecular weight: Translated: 44956; Mature: 44956

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSELNQRRQQATPRGVGVMCNYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPK
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHH
VVAAVAEQLQAFTHTAYQIVPYESYVSLAERINDLAPIDGPAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHH
ARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGPFPGSVYHGVYPNAAHGVTTA
HHHHCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHH
DALKSLERIFKADIAPDQVAAIILEPIQGEGGFNVAPADFMQALRDLCDTHGILLIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCH
QTGFARTGKLFAMQHYEVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGN
HHCHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PLAVAAAHAVLDVIAEEQLCQRAEQLGSHLQEVLNQARATCPAIVDVRGRGSMVAVEFND
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECC
PQTGEPSPEFTRQVQQKAQENGLLLLSCGVYGNVIRFLYPLTIPDAQFSKALDILARVLK
CCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
S
C
>Mature Secondary Structure
MKSSELNQRRQQATPRGVGVMCNYFVEKAENATLWDIEGNEVIDFAAGIAVLNTGHRHPK
CCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCCCHH
VVAAVAEQLQAFTHTAYQIVPYESYVSLAERINDLAPIDGPAKTAFFTTGAEAVENAVKI
HHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHHHHHHH
ARAYTGRPGLITFGGGFHGRTFMTMALTGKVAPYKIGFGPFPGSVYHGVYPNAAHGVTTA
HHHHCCCCCEEEECCCCCCCEEEEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCHH
DALKSLERIFKADIAPDQVAAIILEPIQGEGGFNVAPADFMQALRDLCDTHGILLIADEV
HHHHHHHHHHHCCCCHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCH
QTGFARTGKLFAMQHYEVKPDLMTMAKSLAGGFPLSGVVGRAEVMDAPAPGGLGGTYAGN
HHCHHHCCCEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCC
PLAVAAAHAVLDVIAEEQLCQRAEQLGSHLQEVLNQARATCPAIVDVRGRGSMVAVEFND
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEECC
PQTGEPSPEFTRQVQQKAQENGLLLLSCGVYGNVIRFLYPLTIPDAQFSKALDILARVLK
CCCCCCCHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
S
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]