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The map label for this gene is livK [H]

Identifier: 238896917

GI number: 238896917

Start: 4890500

End: 4891696

Strand: Reverse

Name: livK [H]

Synonym: KP1_5155

Alternate gene names: 238896917

Gene position: 4891696-4890500 (Counterclockwise)

Preceding gene: 238896918

Following gene: 238896916

Centisome position: 93.2

GC content: 58.06

Gene sequence:

>1197_bases
ATGGCGTTGTTTAATAAAAGCACAGAATGCTTTTTAACCATAAAAATACAAAACATTTACACCACATCACGAACGGGGTT
CTGCAGCATGAAAAGGAACGCGAAAACCATCGTCGCGGGCATTGTCGCATTAGCGATGTCGCACGCGGCGATGGCCAAGG
ATATTAAGGTCGCCGTGGTCGGCGCGATGTCGGGTCCGGTGGCCCAGTGGGGCGACATGGAGTTCAATGGCGCGCGTCAG
GCGATTAAAGACATCAACGCCAGCGGCGGCATTAAGGGCGACAAGCTGGTGGCCGTCGAATATGACGACGCCTGCGATCC
GAAGCAAGCGGTCGCGGTGGCCAACAAAATCGTCAACGACGGCATTCAGTACGTTATCGGCCACCTGTGCTCCTCTTCCA
CCCAGCCGGCTTCCGACATCTATGAGGATGAAGGCATCCTGATGATCTCCCCCGGGGCGACCAATCCGGAGCTGACCCAG
CGCGGATACCAGTACATCATGCGTACCGCCGGCCTCGACTCCTCGCAGGGGCCGACCGCGGCGAAATATATCGTCGAGAA
AGTGAAACCGCAGCGTATCGCCATCATTCATGACAAGCAGCAGTACGGCGAAGGGCTGGCGCGTTCGGTGCTGGACAACC
TGAAGAAAGCCGGGGCCAACATCGTCTTCTTCGATGGCATCACCGCGGGGGAAAAAGATTTCTCCGCCCTGCTGGCGCGC
CTGAAGAAAGAGAACATCGACTTCGTCTACTACGGCGGCTACTACCCGGAAATGGGCCAGATGCTGCGCCAGGCGCGCTC
CGTCGGGTTGAAAACCGTGTTTATGGGGCCGGAAGGGGTCGGCAACGCCTCGCTGTCCAATATCGCGGGCGCCGCGGCGG
AAGGCATGCTGGTGACAATGCCAAAACGCTACGATCAGGACCCTGCCAACAGCGCCATCGTCAACGCGCTGAAAGCGGAG
AAGAAAGATCCGAGCGGACCGTACGTCTGGATCACCTATGCCGCCGTTCAGTCGCTGGCGCAGGCGATGGACAGAACCGG
CAGCCAGCAGCCGCTGGATTTAATCAACGATTTGAAAGCGCACGGGGCGAAAACCGTGATTGGGCCGCTGACCTGGGATG
AAAAAGGCGATCTGAAGGGATTTGAGTTTGGTGTCTTCCAGTGGCACGCCGATGGCTCTTCGAGCGCGGCGAAATAA

Upstream 100 bases:

>100_bases
AAATACAGAATATTATCTTTGCTTAATCGACTGAAAAATAGAGGTTTTAATCTGTTTTTTAGTGAAAAACTGCGCTAAAC
CATTAAACTCAATGGTAAAA

Downstream 100 bases:

>100_bases
GCGCTGAAGACGAGTTATCCCTCCGCCCGCGAACCGCGGGCGGTTTGAAAAAGGTTACCTTATGTCCGAGCAGTTTCTCT
ATTTTCTGCAGCAGATGTTT

Product: high-affinity branched-chain amino acid transporter periplasmic binding component

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MALFNKSTECFLTIKIQNIYTTSRTGFCSMKRNAKTIVAGIVALAMSHAAMAKDIKVAVVGAMSGPVAQWGDMEFNGARQ
AIKDINASGGIKGDKLVAVEYDDACDPKQAVAVANKIVNDGIQYVIGHLCSSSTQPASDIYEDEGILMISPGATNPELTQ
RGYQYIMRTAGLDSSQGPTAAKYIVEKVKPQRIAIIHDKQQYGEGLARSVLDNLKKAGANIVFFDGITAGEKDFSALLAR
LKKENIDFVYYGGYYPEMGQMLRQARSVGLKTVFMGPEGVGNASLSNIAGAAAEGMLVTMPKRYDQDPANSAIVNALKAE
KKDPSGPYVWITYAAVQSLAQAMDRTGSQQPLDLINDLKAHGAKTVIGPLTWDEKGDLKGFEFGVFQWHADGSSSAAK

Sequences:

>Translated_398_residues
MALFNKSTECFLTIKIQNIYTTSRTGFCSMKRNAKTIVAGIVALAMSHAAMAKDIKVAVVGAMSGPVAQWGDMEFNGARQ
AIKDINASGGIKGDKLVAVEYDDACDPKQAVAVANKIVNDGIQYVIGHLCSSSTQPASDIYEDEGILMISPGATNPELTQ
RGYQYIMRTAGLDSSQGPTAAKYIVEKVKPQRIAIIHDKQQYGEGLARSVLDNLKKAGANIVFFDGITAGEKDFSALLAR
LKKENIDFVYYGGYYPEMGQMLRQARSVGLKTVFMGPEGVGNASLSNIAGAAAEGMLVTMPKRYDQDPANSAIVNALKAE
KKDPSGPYVWITYAAVQSLAQAMDRTGSQQPLDLINDLKAHGAKTVIGPLTWDEKGDLKGFEFGVFQWHADGSSSAAK
>Mature_397_residues
ALFNKSTECFLTIKIQNIYTTSRTGFCSMKRNAKTIVAGIVALAMSHAAMAKDIKVAVVGAMSGPVAQWGDMEFNGARQA
IKDINASGGIKGDKLVAVEYDDACDPKQAVAVANKIVNDGIQYVIGHLCSSSTQPASDIYEDEGILMISPGATNPELTQR
GYQYIMRTAGLDSSQGPTAAKYIVEKVKPQRIAIIHDKQQYGEGLARSVLDNLKKAGANIVFFDGITAGEKDFSALLARL
KKENIDFVYYGGYYPEMGQMLRQARSVGLKTVFMGPEGVGNASLSNIAGAAAEGMLVTMPKRYDQDPANSAIVNALKAEK
KDPSGPYVWITYAAVQSLAQAMDRTGSQQPLDLINDLKAHGAKTVIGPLTWDEKGDLKGFEFGVFQWHADGSSSAAK

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=369, Percent_Identity=89.7018970189702, Blast_Score=690, Evalue=0.0,
Organism=Escherichia coli, GI48994941, Length=369, Percent_Identity=74.2547425474255, Blast_Score=571, Evalue=1e-164,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42713; Mature: 42582

Theoretical pI: Translated: 7.94; Mature: 7.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFNKSTECFLTIKIQNIYTTSRTGFCSMKRNAKTIVAGIVALAMSHAAMAKDIKVAVV
CCCCCCCCEEEEEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
GAMSGPVAQWGDMEFNGARQAIKDINASGGIKGDKLVAVEYDDACDPKQAVAVANKIVND
ECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
GIQYVIGHLCSSSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTA
HHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
AKYIVEKVKPQRIAIIHDKQQYGEGLARSVLDNLKKAGANIVFFDGITAGEKDFSALLAR
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
LKKENIDFVYYGGYYPEMGQMLRQARSVGLKTVFMGPEGVGNASLSNIAGAAAEGMLVTM
HHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCEEEEC
PKRYDQDPANSAIVNALKAEKKDPSGPYVWITYAAVQSLAQAMDRTGSQQPLDLINDLKA
CCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
HGAKTVIGPLTWDEKGDLKGFEFGVFQWHADGSSSAAK
CCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCC
>Mature Secondary Structure 
ALFNKSTECFLTIKIQNIYTTSRTGFCSMKRNAKTIVAGIVALAMSHAAMAKDIKVAVV
CCCCCCCEEEEEEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
GAMSGPVAQWGDMEFNGARQAIKDINASGGIKGDKLVAVEYDDACDPKQAVAVANKIVND
ECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHH
GIQYVIGHLCSSSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTA
HHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCHH
AKYIVEKVKPQRIAIIHDKQQYGEGLARSVLDNLKKAGANIVFFDGITAGEKDFSALLAR
HHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHH
LKKENIDFVYYGGYYPEMGQMLRQARSVGLKTVFMGPEGVGNASLSNIAGAAAEGMLVTM
HHHCCCCEEEECCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHCCEEEEC
PKRYDQDPANSAIVNALKAEKKDPSGPYVWITYAAVQSLAQAMDRTGSQQPLDLINDLKA
CCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCHHHHHHHHHH
HGAKTVIGPLTWDEKGDLKGFEFGVFQWHADGSSSAAK
CCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]