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The map label for this gene is fruB [H]

Identifier: 238896645

GI number: 238896645

Start: 4624968

End: 4625783

Strand: Direct

Name: fruB [H]

Synonym: KP1_4850

Alternate gene names: 238896645

Gene position: 4624968-4625783 (Clockwise)

Preceding gene: 238896644

Following gene: 238896646

Centisome position: 88.12

GC content: 58.09

Gene sequence:

>816_bases
ATGCAACTGACTTCTGCCGATATTTGTTTCGATCGTCAGGTCAAAACGAAAGACGAGGCGCTACAGCGTGTCGTTGCAGC
CCTGACCCATGCCGGACATACGCGGGCAGACTATCTACAAGGCATGCGCGAGCGCGAGGGGCAAATCAGCACCTATCTGG
GCAACGGCATTGCCATTCCCCACGGTACGCCGCACAGTCGCGACGCAGTGCTTAAAACGGGCGTTAAGGTGCTGGCTTGT
CCGCAGGGGGTTGACTGGGGTGAGGAGCAAACCGCCTATCTGATTGTCGGTATCGCGGCTCAGGACAATGAGCATCTCGA
TATTTTGCGCCAGCTCACCCATGCTCTGGGTGATGATCGAGTACCTGAGGCGCTGACGCGCGCTGATACCCCCCAGGCGG
TGCTGGAAATTCTCGCCGGCGACATTCCTCTGCCAGCAGGCGAAGAACCACAACCCGCGCCGCAATTTGACGAAGAGGCC
ACCTTTACCCTGCGTAATCCGCACGGGCTACATGCCCGTCCCAGCGCGGTGCTGGTGAAAGCCGTCAAGCAGTGGCGATC
GCAAATCCGGGTCGAAAACCTTGATACGCGCTCCGCCATTGTTGATGCCAAGAATCTGATGCGCGTCGTATCGCTGGGCG
CCAAACAGGGTCATCGTCTGCACTTTATGGCCAGCGGCAACGATGCGCAGCAGGCGTTAAAAGCCATTGGTGACGCTTTT
AACGCCGGTCTCGGCGAAATTGCCGCCCAGCCGCAGCAGGTCTCCCAGGAAGCCGTCAAAACGAAACGCAGCTGGCTATC
CCGAATATTTAGTTAA

Upstream 100 bases:

>100_bases
TCCGCCTGCGGGGCACTTATGCTGGGATGCTGGCGGCTGTGGCAAAAACGCACTGACAAAGCGACAGCTCTTGCTGGCGC
CATGAAATAAGGAGCAAGCT

Downstream 100 bases:

>100_bases
TTTTCAATCAATTAAAAACGCCCTGTCTTCCTGGTCAGGGCGTTTTTGTCTCGAAAGACGATGCCTTTCACTGCGTTACA
ACACTATTTATCGATGCAGA

Product: fructose-specific PTS family enzyme IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQLTSADICFDRQVKTKDEALQRVVAALTHAGHTRADYLQGMREREGQISTYLGNGIAIPHGTPHSRDAVLKTGVKVLAC
PQGVDWGEEQTAYLIVGIAAQDNEHLDILRQLTHALGDDRVPEALTRADTPQAVLEILAGDIPLPAGEEPQPAPQFDEEA
TFTLRNPHGLHARPSAVLVKAVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGNDAQQALKAIGDAF
NAGLGEIAAQPQQVSQEAVKTKRSWLSRIFS

Sequences:

>Translated_271_residues
MQLTSADICFDRQVKTKDEALQRVVAALTHAGHTRADYLQGMREREGQISTYLGNGIAIPHGTPHSRDAVLKTGVKVLAC
PQGVDWGEEQTAYLIVGIAAQDNEHLDILRQLTHALGDDRVPEALTRADTPQAVLEILAGDIPLPAGEEPQPAPQFDEEA
TFTLRNPHGLHARPSAVLVKAVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGNDAQQALKAIGDAF
NAGLGEIAAQPQQVSQEAVKTKRSWLSRIFS
>Mature_271_residues
MQLTSADICFDRQVKTKDEALQRVVAALTHAGHTRADYLQGMREREGQISTYLGNGIAIPHGTPHSRDAVLKTGVKVLAC
PQGVDWGEEQTAYLIVGIAAQDNEHLDILRQLTHALGDDRVPEALTRADTPQAVLEILAGDIPLPAGEEPQPAPQFDEEA
TFTLRNPHGLHARPSAVLVKAVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGNDAQQALKAIGDAF
NAGLGEIAAQPQQVSQEAVKTKRSWLSRIFS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=141, Percent_Identity=51.063829787234, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790027, Length=142, Percent_Identity=42.2535211267606, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 29519; Mature: 29519

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLTSADICFDRQVKTKDEALQRVVAALTHAGHTRADYLQGMREREGQISTYLGNGIAIP
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEECC
HGTPHSRDAVLKTGVKVLACPQGVDWGEEQTAYLIVGIAAQDNEHLDILRQLTHALGDDR
CCCCCCCHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCC
VPEALTRADTPQAVLEILAGDIPLPAGEEPQPAPQFDEEATFTLRNPHGLHARPSAVLVK
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
AVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGNDAQQALKAIGDAF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
NAGLGEIAAQPQQVSQEAVKTKRSWLSRIFS
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLTSADICFDRQVKTKDEALQRVVAALTHAGHTRADYLQGMREREGQISTYLGNGIAIP
CCCCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEECC
HGTPHSRDAVLKTGVKVLACPQGVDWGEEQTAYLIVGIAAQDNEHLDILRQLTHALGDDR
CCCCCCCHHHHHCCCEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCC
VPEALTRADTPQAVLEILAGDIPLPAGEEPQPAPQFDEEATFTLRNPHGLHARPSAVLVK
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHH
AVKQWRSQIRVENLDTRSAIVDAKNLMRVVSLGAKQGHRLHFMASGNDAQQALKAIGDAF
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHH
NAGLGEIAAQPQQVSQEAVKTKRSWLSRIFS
HCCHHHHHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]