Click here to switch to the map view.

The map label for this gene is evgA [H]

Identifier: 238896575

GI number: 238896575

Start: 4553858

End: 4554472

Strand: Direct

Name: evgA [H]

Synonym: KP1_4772

Alternate gene names: 238896575

Gene position: 4553858-4554472 (Clockwise)

Preceding gene: 238896573

Following gene: 238896576

Centisome position: 86.76

GC content: 51.71

Gene sequence:

>615_bases
ATGAATGCGATAATCATTGACGATCATCCATTGGCACGTATCGCCATTCGCAACCTTCTCGACAGCAACGGTATTACCGT
CGCGGCAGAGCTCGACAGCGGCGCTCATGCCGTGCAGACCGCGGAAAGCATGCAGCCTGACCTGCTCATCGTCGACGTCG
ATATTCCGGAGCTCAGCGGTATCGAGGTGCTGGAGCAGCTGCGCAAACGCCGCTACCAGGGAACAATCATCATCATTTCC
GCTAAGAATGAACTGTTCTACGGCAAACGCAGCGCCGACTGCGGAGCAAATGGTTTTGTGAGTAAAAAAGAGGGAATGAA
TAATATCCTCGCCGCCATCGACGCAGCCAATAATGGCTACAGCTATTTCCCCTTTTCACTGGAACGCTTCTGCACCCACG
GCATCACCGATCAGGACCGGCTGGATACGCTGTCAACGCAGGAGATGAAGGTGTTCCGCTATATTCTCAGCGGCGTCGAC
TACACCACCATCGGCAGCAAAATGAATATCAGCAATAAAACGGTAAGTACCTATAAGGTCCGTCTGATGGATAAATTAGG
CTGCAGCACCCTGCTTGAGCTATATGACTTTGCGCAACGCAATAAAATAGGGTGA

Upstream 100 bases:

>100_bases
GTGTTACAATTACGCCTTATAACGGCATCGATAAAAATAACCCCCGCCGCACCTGCACCAGGCATCGCCCTTCTTTTTTC
ATATCAGGATTTACGCCCCC

Downstream 100 bases:

>100_bases
TGGCATGGCGGTTCGTCTTATTTTATCCGTCGTTGTCGGGCTGGCGCTGAGCCTCTCGCCTGCCGCGTATGCCATGAAAC
AGCTGGAGCTGAAGAGCCAT

Product: DNA-binding transcriptional activator EvgA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MNAIIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSGIEVLEQLRKRRYQGTIIIIS
AKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAANNGYSYFPFSLERFCTHGITDQDRLDTLSTQEMKVFRYILSGVD
YTTIGSKMNISNKTVSTYKVRLMDKLGCSTLLELYDFAQRNKIG

Sequences:

>Translated_204_residues
MNAIIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSGIEVLEQLRKRRYQGTIIIIS
AKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAANNGYSYFPFSLERFCTHGITDQDRLDTLSTQEMKVFRYILSGVD
YTTIGSKMNISNKTVSTYKVRLMDKLGCSTLLELYDFAQRNKIG
>Mature_204_residues
MNAIIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSGIEVLEQLRKRRYQGTIIIIS
AKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAANNGYSYFPFSLERFCTHGITDQDRLDTLSTQEMKVFRYILSGVD
YTTIGSKMNISNKTVSTYKVRLMDKLGCSTLLELYDFAQRNKIG

Specific function: Member of the two-component regulatory system evgS/evgA. Regulates the expression of emrKY operon and yfdX. Seems also to control expression of at least one other multidrug efflux operon [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=66.6666666666667, Blast_Score=277, Evalue=3e-76,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=31.8627450980392, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788222, Length=206, Percent_Identity=25.7281553398058, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1788521, Length=194, Percent_Identity=28.8659793814433, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI1790102, Length=191, Percent_Identity=28.7958115183246, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=26.3736263736264, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788546, Length=205, Percent_Identity=26.8292682926829, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22635; Mature: 22635

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSG
CCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCC
IEVLEQLRKRRYQGTIIIISAKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAANNGY
HHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHCHHHHHHHHCCCCCCC
SYFPFSLERFCTHGITDQDRLDTLSTQEMKVFRYILSGVDYTTIGSKMNISNKTVSTYKV
EECCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCCCEEECCCEECCCCCEEHHHHE
RLMDKLGCSTLLELYDFAQRNKIG
EHHHHHCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNAIIIDDHPLARIAIRNLLDSNGITVAAELDSGAHAVQTAESMQPDLLIVDVDIPELSG
CCEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEECCCCCCC
IEVLEQLRKRRYQGTIIIISAKNELFYGKRSADCGANGFVSKKEGMNNILAAIDAANNGY
HHHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCCCCHHCHHHHHHHHCCCCCCC
SYFPFSLERFCTHGITDQDRLDTLSTQEMKVFRYILSGVDYTTIGSKMNISNKTVSTYKV
EECCCCHHHHHHCCCCCHHHHHHCCHHHHHHHHHHHCCCCEEECCCEECCCCCEEHHHHE
RLMDKLGCSTLLELYDFAQRNKIG
EHHHHHCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]