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The map label for this gene is ycaN [H]

Identifier: 238896421

GI number: 238896421

Start: 4400500

End: 4401399

Strand: Reverse

Name: ycaN [H]

Synonym: KP1_4596

Alternate gene names: 238896421

Gene position: 4401399-4400500 (Counterclockwise)

Preceding gene: 238896426

Following gene: 238896420

Centisome position: 83.86

GC content: 54.44

Gene sequence:

>900_bases
ATGAATAACGCGCTTTATAATCAGATACGCATCTTTCAGAGCATTGCGCGCGAGGGCAATATCTCGGCCGCCGCGCGCAA
GCTCGAAATCACGCCGCCGTCGGTCAGCAATGCGCTCAGGCTGCTGGAGGAGCATATTGGCCACTCCCTTTTTGTGCGCA
CTACCCGGCGTATCGAGCTGACGGAAACCGGGCAACAGCTGCTGGAGCAGACCGCCGCGGCGGTGGAGACACTGGAAAAG
TCGCTGGAAATCATCCGCGACCAGAATCAGGAGCCCTCCGGGGCAGTGAGAATAACCCTCTCGCGCTTTGCCTACCTGCT
TATTCTCAAACCGGCGATGGCGGCATTTTGTCAGCAATATCCGGGGATCCAGCTGGAAATATCGGTCTATGACGGTACCG
TCAATATTATTGAAGAACGTTTCGACCTTGGGATCCGCTTTGGCGATATTCTCGAAGGCGGCGTGGTCGCCCGCCCGTTA
ATGAAGCCGTTTCGTGAAGGGCTATACGCCTCCTCTGCCTATCTTGCCGAATATGGCGTGCCCGCGATGCCTGCCGATCT
CCATCATCACCGGCTGATTGGCTATCGCTTTATTACCAACAACCGCATCCTGCCGCTGATCCTCAATGACCACGGCGAGC
AGCTCACCGTCGAGATGCCCGGACAGTTAATCAGCAATGATATTGACGTCATGGCCGACGGTATTCGCCACGGACTGGGG
ATTGGCCGTTTATTTGAACCAATCCTGCAATTACAGCCCGATCGTGAGCAGTTTATTCCCGTGATGGAGGACTACTGGAA
AACCTATCCGCCGGTTTATCTCTATTACCCAAAAAACGCGGGCAGAACGAAAAGGGTGAAAGCCCTGATTGATTTCCTGA
TAATGCATGCCGCGGAATAA

Upstream 100 bases:

>100_bases
AAAGTATACGAGCCGCAGGGGGGGAAGATTAGGCGGTCATTGACTTAAGAATATTTAGCCTGTACTTAATAATCAGGCGA
GGGTGTCTTAAGGAGACGCT

Downstream 100 bases:

>100_bases
AGGCTATTTTGCGTGCGAAATATCTGAAGGCACAGATCCTGTCTTCCCTCAGTCTTAACCCTATACTCCTTGCGAATAAA
GCGGTTTTTATCGCGATGAT

Product: putative LysR-family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALRLLEEHIGHSLFVRTTRRIELTETGQQLLEQTAAAVETLEK
SLEIIRDQNQEPSGAVRITLSRFAYLLILKPAMAAFCQQYPGIQLEISVYDGTVNIIEERFDLGIRFGDILEGGVVARPL
MKPFREGLYASSAYLAEYGVPAMPADLHHHRLIGYRFITNNRILPLILNDHGEQLTVEMPGQLISNDIDVMADGIRHGLG
IGRLFEPILQLQPDREQFIPVMEDYWKTYPPVYLYYPKNAGRTKRVKALIDFLIMHAAE

Sequences:

>Translated_299_residues
MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALRLLEEHIGHSLFVRTTRRIELTETGQQLLEQTAAAVETLEK
SLEIIRDQNQEPSGAVRITLSRFAYLLILKPAMAAFCQQYPGIQLEISVYDGTVNIIEERFDLGIRFGDILEGGVVARPL
MKPFREGLYASSAYLAEYGVPAMPADLHHHRLIGYRFITNNRILPLILNDHGEQLTVEMPGQLISNDIDVMADGIRHGLG
IGRLFEPILQLQPDREQFIPVMEDYWKTYPPVYLYYPKNAGRTKRVKALIDFLIMHAAE
>Mature_299_residues
MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALRLLEEHIGHSLFVRTTRRIELTETGQQLLEQTAAAVETLEK
SLEIIRDQNQEPSGAVRITLSRFAYLLILKPAMAAFCQQYPGIQLEISVYDGTVNIIEERFDLGIRFGDILEGGVVARPL
MKPFREGLYASSAYLAEYGVPAMPADLHHHRLIGYRFITNNRILPLILNDHGEQLTVEMPGQLISNDIDVMADGIRHGLG
IGRLFEPILQLQPDREQFIPVMEDYWKTYPPVYLYYPKNAGRTKRVKALIDFLIMHAAE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787128, Length=282, Percent_Identity=27.3049645390071, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787589, Length=272, Percent_Identity=28.3088235294118, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=28.6195286195286, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI87081978, Length=305, Percent_Identity=27.5409836065574, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=27.9310344827586, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789639, Length=295, Percent_Identity=23.728813559322, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1789440, Length=295, Percent_Identity=25.0847457627119, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788706, Length=149, Percent_Identity=31.5436241610738, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI145693105, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786448, Length=150, Percent_Identity=31.3333333333333, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787601, Length=134, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=303, Percent_Identity=26.4026402640264, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=29.375, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788748, Length=213, Percent_Identity=28.169014084507, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789173, Length=142, Percent_Identity=28.169014084507, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33857; Mature: 33857

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALRLLEEHIGHSLFVRTTRRIEL
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHHHHHCCEEEEEEECEEEE
TETGQQLLEQTAAAVETLEKSLEIIRDQNQEPSGAVRITLSRFAYLLILKPAMAAFCQQY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHC
PGIQLEISVYDGTVNIIEERFDLGIRFGDILEGGVVARPLMKPFREGLYASSAYLAEYGV
CCCEEEEEEECCHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PAMPADLHHHRLIGYRFITNNRILPLILNDHGEQLTVEMPGQLISNDIDVMADGIRHGLG
CCCCCCCCHHEEEEEEEEECCCEEEEEECCCCCEEEEECCHHHHHCCHHHHHHHHHHCCC
IGRLFEPILQLQPDREQFIPVMEDYWKTYPPVYLYYPKNAGRTKRVKALIDFLIMHAAE
HHHHHHHHHHCCCCHHHCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNNALYNQIRIFQSIAREGNISAAARKLEITPPSVSNALRLLEEHIGHSLFVRTTRRIEL
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCHHHHHHHHHHHHCCEEEEEEECEEEE
TETGQQLLEQTAAAVETLEKSLEIIRDQNQEPSGAVRITLSRFAYLLILKPAMAAFCQQY
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHC
PGIQLEISVYDGTVNIIEERFDLGIRFGDILEGGVVARPLMKPFREGLYASSAYLAEYGV
CCCEEEEEEECCHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
PAMPADLHHHRLIGYRFITNNRILPLILNDHGEQLTVEMPGQLISNDIDVMADGIRHGLG
CCCCCCCCHHEEEEEEEEECCCEEEEEECCCCCEEEEECCHHHHHCCHHHHHHHHHHCCC
IGRLFEPILQLQPDREQFIPVMEDYWKTYPPVYLYYPKNAGRTKRVKALIDFLIMHAAE
HHHHHHHHHHCCCCHHHCCCHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]