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The map label for this gene is ascG [H]

Identifier: 238896360

GI number: 238896360

Start: 4340759

End: 4341769

Strand: Direct

Name: ascG [H]

Synonym: KP1_4525

Alternate gene names: 238896360

Gene position: 4340759-4341769 (Clockwise)

Preceding gene: 238896355

Following gene: 238896362

Centisome position: 82.7

GC content: 59.05

Gene sequence:

>1011_bases
ATGGCTACAATGCTGGATGTCTCAATTCGCGCAGGCGTCTCGAAAGCGACGGTTTCCCGCGTGTTGAACGGCACAGGTCA
GGTGAAAGAGAGCACCCGCCAACAGGTCTTTAAAGCGATGGAAGAGCTGGGCTACCGGCCCAATTTCCTTGCCCGCTCGC
TGGCCAATCAGACCAGCAATAGTATCGGGCTGGTCGTCTCCACCTTTGACGGCTTTTACTTTGGCCGTCTGCTGCAGCAG
GCGTCACGCCAGACCGAAGCCTGGGGAAAGCAGCTTATCGTCACCGACGGCCATGATACGCCGGAGCGCGAAGAGGAAGC
GGTGCAGATGCTCGCCGACCGGCAGTGCGATGCGATCATCCTCTATACCCGGCACATGAGCGAGAAGGCGATCATGTCGC
TGATTAACTCGATCGCCATGCCGCTGGTGGTGATTAACCGCGATGTGGCGCAGGCCCGCGAACGCTGCGTGTTCTTCGAA
CAGCAGGAAGCCGCCTTCCAGGCCGTGGAGTATCTGATTACCCAGGGCCACCGCGATATCGCCTGCATCACCGTCCCGAT
GCATACCCCTACCGGCCAGGCTCGCCTGCAGGGCTACCGCAACGCGCTGATAAAACATGGTATTGAGTGGGATCCGAGCC
GGGTGAAGTATGGGGACTCGACGATGACTCGCGGCTACGAACTGTGCCGGGAACTGCTGGACGAGAAAGCCCGCTTCAGC
GCGCTGTTTTCCTGTAACGATGATATGGCGCTGGGGGCGTCAAAGGCGCTGCATCAGGCGGGGCTGCGTATTCCACAGGA
TGTGTCGCTGTTTGGCTTCGACGATGCGCCAAGCGCGAAATGGCTGGAGCCGGGGCTGTCGACGGTTTATCTGCCGATCG
ACAACATGATCACTACGGCTATCGACCAGGCGATCAAGCTTGCCAATCAGCAGCCGATAGAAACGATCCCGCCGTTTACC
GGCACGCTGGTGCTTCGCGAGTCGGTGACGACGGGACCTTTTTTTAAATAA

Upstream 100 bases:

>100_bases
AAAGCGTGATCGCGATGGTTTTTCCGCCCCGCCCCGTCGATATGTCCCTTTTCGGTCTGTGATAAACTGCGCTTTACCAC
GTGAAATCAGGAATCGATGA

Downstream 100 bases:

>100_bases
GCTCTAAGGCCAGCAGCTCCTGGATGGTCTGGCGACGGCGGATCAGCCGCGCCTTACCGCCATCAAACAGCACCTCCGGC
AGCAGCGGACGGCTGTTATA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Cryptic asc operon repressor [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MATMLDVSIRAGVSKATVSRVLNGTGQVKESTRQQVFKAMEELGYRPNFLARSLANQTSNSIGLVVSTFDGFYFGRLLQQ
ASRQTEAWGKQLIVTDGHDTPEREEEAVQMLADRQCDAIILYTRHMSEKAIMSLINSIAMPLVVINRDVAQARERCVFFE
QQEAAFQAVEYLITQGHRDIACITVPMHTPTGQARLQGYRNALIKHGIEWDPSRVKYGDSTMTRGYELCRELLDEKARFS
ALFSCNDDMALGASKALHQAGLRIPQDVSLFGFDDAPSAKWLEPGLSTVYLPIDNMITTAIDQAIKLANQQPIETIPPFT
GTLVLRESVTTGPFFK

Sequences:

>Translated_336_residues
MATMLDVSIRAGVSKATVSRVLNGTGQVKESTRQQVFKAMEELGYRPNFLARSLANQTSNSIGLVVSTFDGFYFGRLLQQ
ASRQTEAWGKQLIVTDGHDTPEREEEAVQMLADRQCDAIILYTRHMSEKAIMSLINSIAMPLVVINRDVAQARERCVFFE
QQEAAFQAVEYLITQGHRDIACITVPMHTPTGQARLQGYRNALIKHGIEWDPSRVKYGDSTMTRGYELCRELLDEKARFS
ALFSCNDDMALGASKALHQAGLRIPQDVSLFGFDDAPSAKWLEPGLSTVYLPIDNMITTAIDQAIKLANQQPIETIPPFT
GTLVLRESVTTGPFFK
>Mature_335_residues
ATMLDVSIRAGVSKATVSRVLNGTGQVKESTRQQVFKAMEELGYRPNFLARSLANQTSNSIGLVVSTFDGFYFGRLLQQA
SRQTEAWGKQLIVTDGHDTPEREEEAVQMLADRQCDAIILYTRHMSEKAIMSLINSIAMPLVVINRDVAQARERCVFFEQ
QEAAFQAVEYLITQGHRDIACITVPMHTPTGQARLQGYRNALIKHGIEWDPSRVKYGDSTMTRGYELCRELLDEKARFSA
LFSCNDDMALGASKALHQAGLRIPQDVSLFGFDDAPSAKWLEPGLSTVYLPIDNMITTAIDQAIKLANQQPIETIPPFTG
TLVLRESVTTGPFFK

Specific function: Repressor of the asc operon. The cryptic operon is activated by the insertion of IS186 into the ascG gene [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789068, Length=331, Percent_Identity=42.2960725075529, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1789202, Length=333, Percent_Identity=35.4354354354354, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787948, Length=340, Percent_Identity=33.5294117647059, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788474, Length=336, Percent_Identity=34.8214285714286, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=31.4102564102564, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI1790369, Length=291, Percent_Identity=31.9587628865979, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI48994940, Length=291, Percent_Identity=29.8969072164948, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=27.7439024390244, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=24.9211356466877, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790715, Length=291, Percent_Identity=25.7731958762887, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787906, Length=283, Percent_Identity=27.9151943462898, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790689, Length=289, Percent_Identity=26.9896193771626, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789456, Length=331, Percent_Identity=25.6797583081571, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786268, Length=273, Percent_Identity=25.6410256410256, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1790192, Length=278, Percent_Identity=23.7410071942446, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37400; Mature: 37269

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMLDVSIRAGVSKATVSRVLNGTGQVKESTRQQVFKAMEELGYRPNFLARSLANQTSN
CCCEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SIGLVVSTFDGFYFGRLLQQASRQTEAWGKQLIVTDGHDTPEREEEAVQMLADRQCDAII
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LYTRHMSEKAIMSLINSIAMPLVVINRDVAQARERCVFFEQQEAAFQAVEYLITQGHRDI
EEEHHCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ACITVPMHTPTGQARLQGYRNALIKHGIEWDPSRVKYGDSTMTRGYELCRELLDEKARFS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
ALFSCNDDMALGASKALHQAGLRIPQDVSLFGFDDAPSAKWLEPGLSTVYLPIDNMITTA
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEEHHHHHHHH
IDQAIKLANQQPIETIPPFTGTLVLRESVTTGPFFK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
ATMLDVSIRAGVSKATVSRVLNGTGQVKESTRQQVFKAMEELGYRPNFLARSLANQTSN
CCEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
SIGLVVSTFDGFYFGRLLQQASRQTEAWGKQLIVTDGHDTPEREEEAVQMLADRQCDAII
CCEEEEECHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
LYTRHMSEKAIMSLINSIAMPLVVINRDVAQARERCVFFEQQEAAFQAVEYLITQGHRDI
EEEHHCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
ACITVPMHTPTGQARLQGYRNALIKHGIEWDPSRVKYGDSTMTRGYELCRELLDEKARFS
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHH
ALFSCNDDMALGASKALHQAGLRIPQDVSLFGFDDAPSAKWLEPGLSTVYLPIDNMITTA
HHHCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHCCCCCEEEEEHHHHHHHH
IDQAIKLANQQPIETIPPFTGTLVLRESVTTGPFFK
HHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]