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The map label for this gene is galR [H]

Identifier: 238896355

GI number: 238896355

Start: 4335402

End: 4336418

Strand: Direct

Name: galR [H]

Synonym: KP1_4519

Alternate gene names: 238896355

Gene position: 4335402-4336418 (Clockwise)

Preceding gene: 238896351

Following gene: 238896360

Centisome position: 82.6

GC content: 63.23

Gene sequence:

>1017_bases
ATGGCGACTATTAAGGATGTAGCCCGACTCGCAGGAGTCTCCGTCGCTACCGTCTCCCGCGTCATCAACAACTCTCCCAA
AGCCAGCGAAGCCTCGCGTCAGTCCGTGGGCGCGGCGATGGAAACCCTGAACTATCACCCCAACGCGAACGCCCGGGCGC
TGGCCCAGCAGTCAACGGAAACCGTCGGCCTGGTGGTGGGCGATGTCTCCGACCCCTTCTTCGGCGCGATGGTCAAAGCC
GTCGAACAGGTGGCCTACCGCACCGGCAATTTTTTGCTGATTGGCAATGGCTACCACAACGTGCAGAAGGAGCGCCAGGC
GATAGAGCAGCTGATCCGCCATCGCTGCGCGGCGCTGGTGGTGCACGCCAAAATGATCCCCGATGAGGAACTGGCGGGGT
TAATGAAACAGATCCCCGGCATGGTGCTGATCAACCGCATCCTGCCCGGATATGAAACCCGCTGCGTGGCGCTGGACGAC
CGCTACGGCGCCTGGCTGGCGACCCGCCATTTGATCCAGCAGGGGCACACGCGTATCGGCTATCTGTGCTCAAACCATGA
TATCTCCGATGCGGAAGATCGGCTGCAGGGGTACTACGCGGCGCTGGAGGAGAGCGGCCTGCCGTGCAACGACCGGCTGG
TCACGTTTGCCGAGCCGGATGAAAGCGGCGGCGAACAGGCGATGACCGAACTGCTTGGCCGCGGCCGCCACTTTAGCGCC
GTCGCCTGCTATAACGACTCGATGGCCGCCGGCGCGATGGGGGTGCTGAATGACAATGGCATCGACGTTCCGCGGGAGAT
TTCGCTGATTGGCTTCGACGATGTCCTGATTTCGCGCTATGTCCGCCCGCGGTTGACTACCGTCCGCTATCCGATCGTCA
CGATGGCGACCCAGGCGGCCGAGCTGGCTTTAGCCCTGGCGGAGCAGCGGCCGGCGCCGGAGATCACCCATCTGTTCAGC
CCGACGCTGGTCCGTCGTCACTCGGTGGTCGCCCCGCAGGAAGGCGATAAGTCATAG

Upstream 100 bases:

>100_bases
GAATCACATTTGCGCCATTCCTTCGCCGCCGCGCCTTGAATCTGCCGCTCTTTCCCGTAACATAGCAGTATGTAAACGCT
TACCCTAGAAAAGGCATGGT

Downstream 100 bases:

>100_bases
CGATAGAGGTGCACGGCGGTGGCCGGGTAATCCAGCCCCTCGCCGATGTAGCGCCAGCCGAAGCGTTCATAGAAATCCCG
GCAGGCGGAGTAGAGATAGA

Product: DNA-binding transcriptional regulator GalR

Products: NA

Alternate protein names: Galactose operon repressor [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRQSVGAAMETLNYHPNANARALAQQSTETVGLVVGDVSDPFFGAMVKA
VEQVAYRTGNFLLIGNGYHNVQKERQAIEQLIRHRCAALVVHAKMIPDEELAGLMKQIPGMVLINRILPGYETRCVALDD
RYGAWLATRHLIQQGHTRIGYLCSNHDISDAEDRLQGYYAALEESGLPCNDRLVTFAEPDESGGEQAMTELLGRGRHFSA
VACYNDSMAAGAMGVLNDNGIDVPREISLIGFDDVLISRYVRPRLTTVRYPIVTMATQAAELALALAEQRPAPEITHLFS
PTLVRRHSVVAPQEGDKS

Sequences:

>Translated_338_residues
MATIKDVARLAGVSVATVSRVINNSPKASEASRQSVGAAMETLNYHPNANARALAQQSTETVGLVVGDVSDPFFGAMVKA
VEQVAYRTGNFLLIGNGYHNVQKERQAIEQLIRHRCAALVVHAKMIPDEELAGLMKQIPGMVLINRILPGYETRCVALDD
RYGAWLATRHLIQQGHTRIGYLCSNHDISDAEDRLQGYYAALEESGLPCNDRLVTFAEPDESGGEQAMTELLGRGRHFSA
VACYNDSMAAGAMGVLNDNGIDVPREISLIGFDDVLISRYVRPRLTTVRYPIVTMATQAAELALALAEQRPAPEITHLFS
PTLVRRHSVVAPQEGDKS
>Mature_337_residues
ATIKDVARLAGVSVATVSRVINNSPKASEASRQSVGAAMETLNYHPNANARALAQQSTETVGLVVGDVSDPFFGAMVKAV
EQVAYRTGNFLLIGNGYHNVQKERQAIEQLIRHRCAALVVHAKMIPDEELAGLMKQIPGMVLINRILPGYETRCVALDDR
YGAWLATRHLIQQGHTRIGYLCSNHDISDAEDRLQGYYAALEESGLPCNDRLVTFAEPDESGGEQAMTELLGRGRHFSAV
ACYNDSMAAGAMGVLNDNGIDVPREISLIGFDDVLISRYVRPRLTTVRYPIVTMATQAAELALALAEQRPAPEITHLFSP
TLVRRHSVVAPQEGDKS

Specific function: Repressor of the galactose operon. Binds galactose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=332, Percent_Identity=89.7590361445783, Blast_Score=602, Evalue=1e-174,
Organism=Escherichia coli, GI1788474, Length=335, Percent_Identity=55.8208955223881, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1789068, Length=337, Percent_Identity=35.0148367952522, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=32.1321321321321, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1790369, Length=303, Percent_Identity=32.013201320132, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=32.4242424242424, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1787906, Length=326, Percent_Identity=29.7546012269939, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=343, Percent_Identity=28.2798833819242, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787580, Length=320, Percent_Identity=26.5625, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=25.7328990228013, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI48994940, Length=314, Percent_Identity=26.1146496815287, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1790689, Length=321, Percent_Identity=26.4797507788162, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1786268, Length=325, Percent_Identity=24.9230769230769, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36808; Mature: 36677

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIKDVARLAGVSVATVSRVINNSPKASEASRQSVGAAMETLNYHPNANARALAQQSTE
CCCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
TVGLVVGDVSDPFFGAMVKAVEQVAYRTGNFLLIGNGYHNVQKERQAIEQLIRHRCAALV
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VHAKMIPDEELAGLMKQIPGMVLINRILPGYETRCVALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
YLCSNHDISDAEDRLQGYYAALEESGLPCNDRLVTFAEPDESGGEQAMTELLGRGRHFSA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPREISLIGFDDVLISRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALAEQRPAPEITHLFSPTLVRRHSVVAPQEGDKS
HHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ATIKDVARLAGVSVATVSRVINNSPKASEASRQSVGAAMETLNYHPNANARALAQQSTE
CCHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
TVGLVVGDVSDPFFGAMVKAVEQVAYRTGNFLLIGNGYHNVQKERQAIEQLIRHRCAALV
EEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
VHAKMIPDEELAGLMKQIPGMVLINRILPGYETRCVALDDRYGAWLATRHLIQQGHTRIG
HHHHCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEE
YLCSNHDISDAEDRLQGYYAALEESGLPCNDRLVTFAEPDESGGEQAMTELLGRGRHFSA
EEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCEEE
VACYNDSMAAGAMGVLNDNGIDVPREISLIGFDDVLISRYVRPRLTTVRYPIVTMATQAA
EEEECCCCCCCEEEEECCCCCCCCCEEEEEEHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
ELALALAEQRPAPEITHLFSPTLVRRHSVVAPQEGDKS
HHHHHHHHCCCCCHHHHHHCHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6283521; 9278503; 6350601; 8188660; 8982002 [H]