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The map label for this gene is csdA [H]

Identifier: 238896282

GI number: 238896282

Start: 4264710

End: 4265951

Strand: Direct

Name: csdA [H]

Synonym: KP1_4441

Alternate gene names: 238896282

Gene position: 4264710-4265951 (Clockwise)

Preceding gene: 238896277

Following gene: 238896283

Centisome position: 81.26

GC content: 65.62

Gene sequence:

>1242_bases
TTGCGCGACGATCGCGGTATTGATACGAGGAATTCCATGAACGCATTCAACCCGGCGCACTTTCGCGCGCAGTTTCCGTC
TCTGGCCGATGCCGGCGTCTATCTCGACAGCGCGGCGACAGCGCTCAAACCGCTGGCGGTGATCGACGCCAGCGACCAGT
TTTATCGCCTGAGCGCCGGCAATGTCCATCGCAGCCAGTTCGCCGCCGCCCAGCGGCTGACCGAGCGCTACGAGGCGGCC
CGCGATCGCGTGGCGGCGTGGCTTAACGCCCCTTCCGGCAAAGATATCGTCTGGACGCGGGGGACCACCGAGGCCATCAA
TATGGTGGCGCAAAGCTACGTCCGGCCGCGCCTGCAGCCTGGCGATGAAATTATCGTTAGCGAAGCCGAGCATCATGCCA
ACCTCGTGCCCTGGCTGATGGTGGCCGCGCAGACCGGCGCCAGAGTGGTCAAACTGCCGCTTGGCGCCGACCGCCTGCCA
GATATCGCCAGCCTCAGCGCGCTGATTACCCCGCGCAGCCGGGTGCTGGCCATCGGCCAGATGTCCAACGTCACCGGCGG
CTGCCCGGACCTGGCGCAGGCTATCCGCCTTGCCCACGCCGCCGGGATGGTGGTGATGGTTGATGGCGCCCAGGGGGCGG
TGCATTTCCCCGCTGACGTGCAGGCGCTGGATATCGATTTTTACGCCTTTTCCGGCCATAAGCTGTATGGCCCGACCGGG
ATCGGCGCCCTGTACGGCAAAAGCGAGCTGCTGGCAGCGATGTCGCCCTGGCTCGGCGGCGGGAAAATGATCGCCGAGGT
CAGCTTTGACGGCTTTACGCCGCAGCCGGCCCCCTATGGCCTCGAGGCCGGTACGCCGAATGTCGCCGGGGTTATCGGCC
TCAGCGCCGCGCTGGAGTGGCTGGCGCAGAGCGATATCGGCCAGGCGGAAAACTGGAGCCGCAGTCTGGCGAGCCTGGCG
GAAGAGGAACTGGCGAAACGGCCGGGCTTCCGCTCTTTCCGCTGCCAGCAGTCAAGCCTGCTGGCCTTTGAATTTGAGGG
TATTCATCACAGCGATCTGGTGACGCTGCTGGCGGAGTCCGGCATTGCCCTGCGCGCCGGGCAACACTGCGCCCAGCCGC
TGCTGGCGGCCTTAGGGGTCAGCGGCACCCTGCGGGCCTCTTTCGCCCCCTACAATACCCAAGACGATGTTGCGGCGCTG
GTCCACGCGGTGGATCGCGCCCTGGAAATTCTGGTGGATTAA

Upstream 100 bases:

>100_bases
AACACAAACCCAACACTTTTCAGCATAACCCTCTTTGACCGACGTGGAGTCACATTCGGACAATTCCACAGGACGCAGAG
ACCTTGTCACGCCCGGCAAA

Downstream 100 bases:

>100_bases
TGACAACTCTGCACCCCTTCGGCACAACCATTACCGACGCTACGCTGCGCCAGATCTTCGCCCCGCTGAACCAGTGGGAG
GATAAATACCGTCAGCTGAT

Product: cysteine sulfinate desulfinase

Products: @ALAN01txt*L-Alanine! [C]

Alternate protein names: CSD [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MRDDRGIDTRNSMNAFNPAHFRAQFPSLADAGVYLDSAATALKPLAVIDASDQFYRLSAGNVHRSQFAAAQRLTERYEAA
RDRVAAWLNAPSGKDIVWTRGTTEAINMVAQSYVRPRLQPGDEIIVSEAEHHANLVPWLMVAAQTGARVVKLPLGADRLP
DIASLSALITPRSRVLAIGQMSNVTGGCPDLAQAIRLAHAAGMVVMVDGAQGAVHFPADVQALDIDFYAFSGHKLYGPTG
IGALYGKSELLAAMSPWLGGGKMIAEVSFDGFTPQPAPYGLEAGTPNVAGVIGLSAALEWLAQSDIGQAENWSRSLASLA
EEELAKRPGFRSFRCQQSSLLAFEFEGIHHSDLVTLLAESGIALRAGQHCAQPLLAALGVSGTLRASFAPYNTQDDVAAL
VHAVDRALEILVD

Sequences:

>Translated_413_residues
MRDDRGIDTRNSMNAFNPAHFRAQFPSLADAGVYLDSAATALKPLAVIDASDQFYRLSAGNVHRSQFAAAQRLTERYEAA
RDRVAAWLNAPSGKDIVWTRGTTEAINMVAQSYVRPRLQPGDEIIVSEAEHHANLVPWLMVAAQTGARVVKLPLGADRLP
DIASLSALITPRSRVLAIGQMSNVTGGCPDLAQAIRLAHAAGMVVMVDGAQGAVHFPADVQALDIDFYAFSGHKLYGPTG
IGALYGKSELLAAMSPWLGGGKMIAEVSFDGFTPQPAPYGLEAGTPNVAGVIGLSAALEWLAQSDIGQAENWSRSLASLA
EEELAKRPGFRSFRCQQSSLLAFEFEGIHHSDLVTLLAESGIALRAGQHCAQPLLAALGVSGTLRASFAPYNTQDDVAAL
VHAVDRALEILVD
>Mature_413_residues
MRDDRGIDTRNSMNAFNPAHFRAQFPSLADAGVYLDSAATALKPLAVIDASDQFYRLSAGNVHRSQFAAAQRLTERYEAA
RDRVAAWLNAPSGKDIVWTRGTTEAINMVAQSYVRPRLQPGDEIIVSEAEHHANLVPWLMVAAQTGARVVKLPLGADRLP
DIASLSALITPRSRVLAIGQMSNVTGGCPDLAQAIRLAHAAGMVVMVDGAQGAVHFPADVQALDIDFYAFSGHKLYGPTG
IGALYGKSELLAAMSPWLGGGKMIAEVSFDGFTPQPAPYGLEAGTPNVAGVIGLSAALEWLAQSDIGQAENWSRSLASLA
EEELAKRPGFRSFRCQQSSLLAFEFEGIHHSDLVTLLAESGIALRAGQHCAQPLLAALGVSGTLRASFAPYNTQDDVAAL
VHAVDRALEILVD

Specific function: Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L- selenocystine to produce L-alanine. L-cysteine sulfinic acid is the best substrate. Functions as a selenium delivery protein in the pathway f

COG id: COG0520

COG function: function code E; Selenocysteine lyase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. Csd subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=374, Percent_Identity=26.2032085561497, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI156713448, Length=323, Percent_Identity=27.8637770897833, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI1789175, Length=401, Percent_Identity=83.2917705735661, Blast_Score=686, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=403, Percent_Identity=44.4168734491315, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI48994898, Length=399, Percent_Identity=23.8095238095238, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=229, Percent_Identity=28.82096069869, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI193211090, Length=362, Percent_Identity=25.9668508287293, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=26.3157894736842, Blast_Score=100, Evalue=5e-22,
Organism=Drosophila melanogaster, GI20129463, Length=360, Percent_Identity=26.9444444444444, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR022471
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: 4.4.1.- [C]

Molecular weight: Translated: 43963; Mature: 43963

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDDRGIDTRNSMNAFNPAHFRAQFPSLADAGVYLDSAATALKPLAVIDASDQFYRLSAG
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHCCEEEEECCCCEEEECCC
NVHRSQFAAAQRLTERYEAARDRVAAWLNAPSGKDIVWTRGTTEAINMVAQSYVRPRLQP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
GDEIIVSEAEHHANLVPWLMVAAQTGARVVKLPLGADRLPDIASLSALITPRSRVLAIGQ
CCEEEEECCHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHEEEEEC
MSNVTGGCPDLAQAIRLAHAAGMVVMVDGAQGAVHFPADVQALDIDFYAFSGHKLYGPTG
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCC
IGALYGKSELLAAMSPWLGGGKMIAEVSFDGFTPQPAPYGLEAGTPNVAGVIGLSAALEW
CHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LAQSDIGQAENWSRSLASLAEEELAKRPGFRSFRCQQSSLLAFEFEGIHHSDLVTLLAES
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GIALRAGQHCAQPLLAALGVSGTLRASFAPYNTQDDVAALVHAVDRALEILVD
CCEEECCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRDDRGIDTRNSMNAFNPAHFRAQFPSLADAGVYLDSAATALKPLAVIDASDQFYRLSAG
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCEEHHHHHHHHCCEEEEECCCCEEEECCC
NVHRSQFAAAQRLTERYEAARDRVAAWLNAPSGKDIVWTRGTTEAINMVAQSYVRPRLQP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHCCCCCC
GDEIIVSEAEHHANLVPWLMVAAQTGARVVKLPLGADRLPDIASLSALITPRSRVLAIGQ
CCEEEEECCHHHCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCHHEEEEEC
MSNVTGGCPDLAQAIRLAHAAGMVVMVDGAQGAVHFPADVQALDIDFYAFSGHKLYGPTG
CCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCEECCCCCEEEEEEEEEECCCEEECCCC
IGALYGKSELLAAMSPWLGGGKMIAEVSFDGFTPQPAPYGLEAGTPNVAGVIGLSAALEW
CHHHHCHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LAQSDIGQAENWSRSLASLAEEELAKRPGFRSFRCQQSSLLAFEFEGIHHSDLVTLLAES
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHC
GIALRAGQHCAQPLLAALGVSGTLRASFAPYNTQDDVAALVHAVDRALEILVD
CCEEECCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cystine; L-selenocystine [C]

Specific reaction: Catalyzes the removal of elemental sulfur and selenium atoms from l-cysteine, l-cystine, l-selenocysteine, and l- selenocystine to produce @ALAN01.txt*l-Alanine! [C]

General reaction: Remove sulfur and selenium atoms [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278392; 9278503; 10829016; 10739946 [H]