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The map label for this gene is hycE [H]

Identifier: 238896178

GI number: 238896178

Start: 4148794

End: 4150503

Strand: Reverse

Name: hycE [H]

Synonym: KP1_4325

Alternate gene names: 238896178

Gene position: 4150503-4148794 (Counterclockwise)

Preceding gene: 238896179

Following gene: 238896177

Centisome position: 79.08

GC content: 61.17

Gene sequence:

>1710_bases
ATGTCTGAAGAAAAAATCGGTCAACACTACCTTGCGGCGCTGCACCAGGCCTTTCCCGGCGTGGTGCTGGACGAAGCCTG
GCAGACAAAAGACCAGTTAACCATCACCGTGAAGGTCAACTACCTGCCGGAAGTGGTGGAGTTTCTCTACTACCAGCAGG
GCGGCTGGCTGTCGGTGCTGTTCGGCAACGATGAACGTAAGCTCAACGGCCACTACGCGGTCTATTACGTGCTGTCGATG
GAGCAGGGCACCAAATGCTGGATCACCGTGCGCGTGGAAGTGGACGCCAACAAACCGGAATACCCGTCGGTGACGCCGCG
CGTCCCGGCGGCGGTATGGGGCGAGCGCGAAGTGCGTGATATGTACGGCCTGGTGCCGGTCGGCCTGCCGGACGAACGCC
GGCTGGTGCTGCCGGACGACTGGCCGGACGAACTCTATCCGCTGCGCAAAGACAGCATGGATTATCGCCAGCGCCCGGCG
CCGACCACCGACGCCGAAACCTACGAGTTTATTAACGAGCTCGGCAGCAAGAAAAACAACGTGGTGCCGATTGGCCCGCT
GCACGTCACCTCTGATGAACCTGGCCACTTCCGCCTGTTCGTTGACGGCGAGAACATTATCGACGCCGACTACCGCCTGT
TCTACGTCCATCGCGGGATGGAAAAGCTGGCGGAAACCCGCATGGGTTATAACGAAGTGACCTTCCTGTCGGACCGGGTG
TGCGGCATCTGCGGCTTCGCCCACAGCACCGCTTACACCACCTCGGTGGAGAACGCGATGGGCATCGTGGTGCCGGAGCG
GGCGCAGATGATCCGCGCCATTCTGCTGGAGGTGGAACGCCTGCACTCGCACCTGCTGAACCTCGGTCTGGCCTGCCACT
TTACCGGCTTTGACTCCGGCTTTATGCAGTTCTTCCGCGTGCGCGAAACCTCGATGAAGATGGCAGAGATCCTTACCGGC
GCGCGTAAAACCTACGGCCTGAACCTGATCGGCGGGATCCGCCGCGACCTGCTGAAAGAGGACATGATCCAGACCCGTCA
GCTGGCGCAGCAGATGCGTCGCGACGTGCAGGAGCTGGTGGATATGCTGCTCAGCACGCCGAATATGGAACAGCGTACCG
TCGGTATTGGCCGTCTGGACCCGGAAATCGCCCGCGACTTCAGCAACGTCGGCCCGATGGTGCGCGCCAGCGGCCACGCC
CGCGACACCCGCGCCGACCACCCGTTTGTCGGTTACGGCCTGCTGCCGATGGAAGTCCACAGCGAACACGGCTGCGATGT
GATATCTCGCCTCAAGGTGCGGATCAACGAGGTTTACACCGCGCTGAATATGATTGATTTCGGTCTCGATAACCTGCCGG
GCGGCCCACTGATGGTGGAGGGGTTCACCTATATTCCGCACCGCTTCGCGCTGGGCTTCTCCGAGGCGCCGCGCGGCGAC
GATATCCACTGGAGCATGACCGGCGACAACCAGAAACTGTACCGCTGGCGTTGCCGGGCGGCGACTTACGCCAACTGGCC
GACCCTGCGCTATATGCTGCGCGGCAACACCGTTTCCGATGCGCCGCTGATCATCGGCAGCCTCGACCCGTGCTACTCCT
GTACTGACCGCATGACCGTGGTCGATGTACGCAAGAAGAAGAGCAAAGTGGTGCCATACAAAGAGCTGGAGCGCTACAGC
ATCGAGCGTAAAAACTCGCCGCTGAAATAA

Upstream 100 bases:

>100_bases
CTGCGTTTTTGTGCCACCTCCCGCGTCACCTGGGCCGGGTTTGGTTTCGCCTTTTTAGCTTTCGTCTCCTTGCTGGTGGC
GTGATTAAGAGAGTTTGAGC

Downstream 100 bases:

>100_bases
GGAATCGCCATGTTTACCTTTATTAAAAAAGTTATTAAGACCGGCACCGCGACGCACGCCTACCCGCTGGAGCCGATGCC
GGTGGACAAAAACTTTCGCG

Product: hydrogenase 3 large subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 569; Mature: 568

Protein sequence:

>569_residues
MSEEKIGQHYLAALHQAFPGVVLDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERKLNGHYAVYYVLSM
EQGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLVPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGSKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIVVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEHGCDVISRLKVRINEVYTALNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFSEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK

Sequences:

>Translated_569_residues
MSEEKIGQHYLAALHQAFPGVVLDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERKLNGHYAVYYVLSM
EQGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLVPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
PTTDAETYEFINELGSKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
CGICGFAHSTAYTTSVENAMGIVVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTG
ARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHA
RDTRADHPFVGYGLLPMEVHSEHGCDVISRLKVRINEVYTALNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFSEAPRGD
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYS
IERKNSPLK
>Mature_568_residues
SEEKIGQHYLAALHQAFPGVVLDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVLFGNDERKLNGHYAVYYVLSME
QGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRDMYGLVPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAP
TTDAETYEFINELGSKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRVC
GICGFAHSTAYTTSVENAMGIVVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGA
RKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELVDMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHAR
DTRADHPFVGYGLLPMEVHSEHGCDVISRLKVRINEVYTALNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFSEAPRGDD
IHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSI
ERKNSPLK

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=387, Percent_Identity=25.5813953488372, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI260898743, Length=377, Percent_Identity=25.4641909814324, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1789076, Length=569, Percent_Identity=97.1880492091388, Blast_Score=1160, Evalue=0.0,
Organism=Escherichia coli, GI1788832, Length=568, Percent_Identity=71.4788732394366, Blast_Score=864, Evalue=0.0,
Organism=Escherichia coli, GI145693162, Length=563, Percent_Identity=25.5772646536412, Blast_Score=157, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17568379, Length=387, Percent_Identity=25.5813953488372, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17555284, Length=400, Percent_Identity=25, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI221459469, Length=387, Percent_Identity=25.3229974160207, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24638644, Length=389, Percent_Identity=26.4781491002571, Blast_Score=133, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 64979; Mature: 64848

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00535 COMPLEX1_49K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKIGQHYLAALHQAFPGVVLDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVL
CCHHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERKLNGHYAVYYVLSMEQGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRD
ECCCCCEECCEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHH
MYGLVPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGSKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIVVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECEEEEEEC
SEHGCDVISRLKVRINEVYTALNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFSEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHCCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEEKIGQHYLAALHQAFPGVVLDEAWQTKDQLTITVKVNYLPEVVEFLYYQQGGWLSVL
CHHHHHHHHHHHHHHHCCCCEEHHHHCCCCEEEEEEEECCHHHHHHHHHHCCCCEEEEE
FGNDERKLNGHYAVYYVLSMEQGTKCWITVRVEVDANKPEYPSVTPRVPAAVWGEREVRD
ECCCCCEECCEEEEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCCCHHHCCCHHHHH
MYGLVPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPAPTTDAETYEFINELGSKKNN
HHCCCCCCCCCCCEEECCCCCCHHCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCC
VVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAETRMGYNEVTFLSDRV
EEECCCEEECCCCCCEEEEEECCCCEECCCEEEEEEHHHHHHHHHHHCCCCCEEEHHHHH
CGICGFAHSTAYTTSVENAMGIVVPERAQMIRAILLEVERLHSHLLNLGLACHFTGFDSG
HHHHCCCCCCCEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
FMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLLKEDMIQTRQLAQQMRRDVQELV
HHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DMLLSTPNMEQRTVGIGRLDPEIARDFSNVGPMVRASGHARDTRADHPFVGYGLLPMEVH
HHHHCCCCCHHHCCCCCCCCHHHHHHHHHCCCCEECCCCCCCCCCCCCEEEECEEEEEEC
SEHGCDVISRLKVRINEVYTALNMIDFGLDNLPGGPLMVEGFTYIPHRFALGFSEAPRGD
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEECCHHHCCCCCCCCCCC
DIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTV
CEEEEEECCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCCCEEEECCCCHHCCCCCEEE
VDVRKKKSKVVPYKELERYSIERKNSPLK
EEEHHHHCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]