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The map label for this gene is alaS [H]

Identifier: 238896148

GI number: 238896148

Start: 4119244

End: 4121871

Strand: Reverse

Name: alaS [H]

Synonym: KP1_4291

Alternate gene names: 238896148

Gene position: 4121871-4119244 (Counterclockwise)

Preceding gene: 238896149

Following gene: 238896147

Centisome position: 78.53

GC content: 58.94

Gene sequence:

>2628_bases
ATGAGCAAGAGCACCGCTGAGATCCGTCAGGCGTTTCTCGACTTTTTCCATAGCAAGGGACATCAGGTAGTTGCCAGCAG
CTCCCTGGTGCCGCACAACGATCCGACCCTGCTGTTTACCAATGCAGGGATGAACCAGTTCAAGGATGTCTTCCTTGGTC
TCGACAAACGTAATTATTCGCGTGCGACCACCGCACAGCGTTGCGTGCGTGCGGGTGGTAAACACAACGATCTGGAAAAC
GTCGGTTACACCGCGCGTCACCACACCTTCTTTGAAATGCTGGGCAACTTCAGCTTTGGCGATTACTTCAAGCAGGATGC
CATCAAATACGCGTGGGAACTGCTGACCGGTGAGAACTGGTTCGCGCTGCCGAAAGAAAAACTGTGGGTTACCGTTTACG
AGACCGATGACGAAGCTTTCGACATCTGGGCCAATGAAGTAGGCGTTCCGCGCGAGCGTATTATCCGCATAGGCGACAAC
AAAGGCGCGCCGTTTGCTTCAGACAACTTCTGGCAGATGGGCGACACCGGTCCGTGCGGCCCGTGCACCGAAATCTTCTT
TGACCACGGCGATCACATCTGGGGCGGCCCTCCGGGAAGCCCGGAAGAAGATGGCGACCGTTACATTGAGATTTGGAACA
TCGTCTTCATGCAGTTCAACCGTCAGGCTGACGGTACCATGGAGCCGCTGCCGAAGCCGTCCGTCGACACCGGTATGGGT
CTGGAGCGTATTGCCGCGGTGCTGCAGCACGTTAACTCCAACTATGACATCGACCTGTTCCGCGATCTGATCGCCAGCGT
GGCGAAAGTCACCGGCGCGACCGATCTGACGAACAAGTCGCTGCGCGTTATCGCCGACCACATTCGCTCCTGCGCCTTCC
TGGTTGCCGACGGCGTGATCCCGTCGAATGAAAACCGCGGCTACGTGCTGCGTCGTATCATTCGCCGCGCGATCCGTCAC
GGCAACATGCTGGGCGCGAAGGACACCTTCTTCTGGAAACTGGTTGCCCCGCTGATCGATGTGATGGGCTCCGCCGGCGA
CGAGCTGAAGCAGCAGCAGGCTCAGGTTGAGCAGGTGCTGAAAACCGAAGAAGAGCAGTTTGCCCGCACCCTCGAGCGCG
GCCTGGCGCTGCTGGATGAGGAGCTGTCGAAGCTGAAAGGCGACACCCTGGACGGCGAAACCGCCTTCCGTCTGTACGAC
ACCTACGGCTTCCCGGTCGACCTGACCGCCGATGTATGCCGCGAGCGCAACATCAAGGTTGATGAAGCTGGTTTTGAAGC
CGCGATGGAAGAGCAGCGCCGCCGCGCGCGCGAGTCCAGCGGTTTCGGCGCCGACTATAATGCGATGATCCGCGTTGACG
GCGCCTCTGAATTTAAAGGCTATGATCATCTGGAGCTGAACGGCAAGGTTACCGCCCTGTTTATCGACGGCAAAGCGGTG
GATAGCGTTAGCGCTGGCCAGGAAGCGGTGGTGATCCTTGACCAGACCCCGTTCTATGCGGAGTCCGGCGGCCAGGTGGG
CGATAAAGGCGAATTAAAAGGCGCGGGCTTCTCTTTCGCAGTTAGCGACACGCAGAAGTACGGCCAGGCTATTGGTCATA
TCGGTAAAGTCGCCAGCGGCTCACTGAAAGTGGGTGACGCGGTACAGGCCGACGTAGATGAGGCTCGGCGTCAGCGTATC
CGTCTCAACCACTCCGCGACGCACCTGATGCACGCGGCGCTGCGCCAGGTGCTCGGCACCCATGTGGCGCAGAAAGGTTC
GCTGGTCAATGATAAAGCGCTGCGCTTTGACTTCTCGCATTTTGAAGCGATGAAGCCGGAAGAAATTCGTGCGGTGGAAG
ACCTGGTCAACGCGCAGATCCGTCGTAACCTGGCTATCGAAACCAACATTATGGATATCGATGCCGCGCGCGCATCCGGC
GCCATGGCGCTGTTTGGCGAGAAATACGACGATCGCGTGCGCGTTCTGCGGATGGGCGACTTCTCCACGGAGCTGTGCGG
CGGGACTCACGCGGCGCGTACCGGCGATATCGGCCTGTTCCGCATCACGTCAGAGTCCGGCACCGCTGCTGGCGTGCGCC
GTATCGAAGCGGTGACTGGCGAAGGCGCGATGGCCATCCTGCATGCGCAGAGCGATCAGCTCAATGATATCGCTCAGCTG
CTGAAGGGTGACAGCCACAATCTCGGCGAGAAGGTTCGCGCCGCGCTGGAGCGTACCCGTCAGCTGGAAAAAGAGCTGCA
GCAGCTGAAAGAGCAGGCTGCGGCGCAGGAGAGCGCAAACCTCTCCAGCAAAGCGGAAGAGATTAACGGCGTTAAGCTGC
TGGTCAGCGAACTGACCGGCGTTGAGCCGAAAATGCTGCGTACCATGGTTGACGATCTGAAGAACCAGCTCGGTTCGACG
ATCGTTGTATTAGCTACGGTAGCGGATGGTAAGGTTTCTCTGATTGCAGGTGTGTCTAAGGATGTGACAGACCGCGTGAA
AGCAGGGGAGCTGGTTGGTATGGTCGCCCAGCAGGTGGGCGGTAAAGGGGGCGGTCGTCCGGATATGGCGCAGGCCGGTG
GTACCGATGCGTCTGCTCTCCCTGCAGCGTTAGCCAGTGTGAAAGGCTGGGTCAGCGCAAAACTGTAA

Upstream 100 bases:

>100_bases
CTCTCTTTCGCAACGCACGTCAGTTCGGTGCCGGAAGAGCAAGCGCAGCCAGGACAGAGGCGACTTGAAGGATGACGTGT
AGCTTGATTTCAGGATAATT

Downstream 100 bases:

>100_bases
TAACTATAAACGCATTTACCCTAAATAATTCGAGTTGCAGGAAGGCGGCGACGCAGCGAATCCCCAGGAGCTTACTCAAG
TAAGTGACTGGGGTGAGCGA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 875; Mature: 874

Protein sequence:

>875_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTAQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGENWFALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDN
KGAPFASDNFWQMGDTGPCGPCTEIFFDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRDLIASVAKVTGATDLTNKSLRVIADHIRSCAFLVADGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKDTFFWKLVAPLIDVMGSAGDELKQQQAQVEQVLKTEEEQFARTLERGLALLDEELSKLKGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFIDGKAV
DSVSAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFAVSDTQKYGQAIGHIGKVASGSLKVGDAVQADVDEARRQRI
RLNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSHFEAMKPEEIRAVEDLVNAQIRRNLAIETNIMDIDAARASG
AMALFGEKYDDRVRVLRMGDFSTELCGGTHAARTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAILHAQSDQLNDIAQL
LKGDSHNLGEKVRAALERTRQLEKELQQLKEQAAAQESANLSSKAEEINGVKLLVSELTGVEPKMLRTMVDDLKNQLGST
IVVLATVADGKVSLIAGVSKDVTDRVKAGELVGMVAQQVGGKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL

Sequences:

>Translated_875_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTAQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGENWFALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDN
KGAPFASDNFWQMGDTGPCGPCTEIFFDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRDLIASVAKVTGATDLTNKSLRVIADHIRSCAFLVADGVIPSNENRGYVLRRIIRRAIRH
GNMLGAKDTFFWKLVAPLIDVMGSAGDELKQQQAQVEQVLKTEEEQFARTLERGLALLDEELSKLKGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFIDGKAV
DSVSAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFAVSDTQKYGQAIGHIGKVASGSLKVGDAVQADVDEARRQRI
RLNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSHFEAMKPEEIRAVEDLVNAQIRRNLAIETNIMDIDAARASG
AMALFGEKYDDRVRVLRMGDFSTELCGGTHAARTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAILHAQSDQLNDIAQL
LKGDSHNLGEKVRAALERTRQLEKELQQLKEQAAAQESANLSSKAEEINGVKLLVSELTGVEPKMLRTMVDDLKNQLGST
IVVLATVADGKVSLIAGVSKDVTDRVKAGELVGMVAQQVGGKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL
>Mature_874_residues
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTAQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGENWFALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDNK
GAPFASDNFWQMGDTGPCGPCTEIFFDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGL
ERIAAVLQHVNSNYDIDLFRDLIASVAKVTGATDLTNKSLRVIADHIRSCAFLVADGVIPSNENRGYVLRRIIRRAIRHG
NMLGAKDTFFWKLVAPLIDVMGSAGDELKQQQAQVEQVLKTEEEQFARTLERGLALLDEELSKLKGDTLDGETAFRLYDT
YGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFIDGKAVD
SVSAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFAVSDTQKYGQAIGHIGKVASGSLKVGDAVQADVDEARRQRIR
LNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSHFEAMKPEEIRAVEDLVNAQIRRNLAIETNIMDIDAARASGA
MALFGEKYDDRVRVLRMGDFSTELCGGTHAARTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAILHAQSDQLNDIAQLL
KGDSHNLGEKVRAALERTRQLEKELQQLKEQAAAQESANLSSKAEEINGVKLLVSELTGVEPKMLRTMVDDLKNQLGSTI
VVLATVADGKVSLIAGVSKDVTDRVKAGELVGMVAQQVGGKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=979, Percent_Identity=38.6108273748723, Blast_Score=560, Evalue=1e-159,
Organism=Homo sapiens, GI38569417, Length=967, Percent_Identity=35.1602895553258, Blast_Score=483, Evalue=1e-136,
Organism=Escherichia coli, GI1789048, Length=875, Percent_Identity=91.0857142857143, Blast_Score=1659, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=979, Percent_Identity=36.7722165474974, Blast_Score=544, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17536681, Length=742, Percent_Identity=33.5579514824798, Blast_Score=379, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6324911, Length=764, Percent_Identity=42.8010471204189, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI24582809, Length=975, Percent_Identity=36.8205128205128, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI45552267, Length=975, Percent_Identity=36.8205128205128, Blast_Score=559, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24658214, Length=810, Percent_Identity=31.4814814814815, Blast_Score=353, Evalue=3e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95623; Mature: 95492

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGENW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCE
FALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDNKGAPFASDNFWQMGDTGPCG
EECCCCCEEEEEEECCCCHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFFDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRDLIASVAKVTGATDLTNKSLRVIADHIRSCAFLVADGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKDTFFWKLVAPLIDVMGSAGDELKQQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELSKLKGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFIDGKAV
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCCC
DSVSAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFAVSDTQKYGQAIGHIGKVASG
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SLKVGDAVQADVDEARRQRIRLNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSH
CEECCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHH
FEAMKPEEIRAVEDLVNAQIRRNLAIETNIMDIDAARASGAMALFGEKYDDRVRVLRMGD
HHCCCHHHHHHHHHHHHHHHHHCCEEEECEEEEECHHCCCCEEECCCCCCCCEEEEEECC
FSTELCGGTHAARTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAILHAQSDQLNDIAQL
CCHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LKGDSHNLGEKVRAALERTRQLEKELQQLKEQAAAQESANLSSKAEEINGVKLLVSELTG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSTIVVLATVADGKVSLIAGVSKDVTDRVKAGELVGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKQDAIKYAWELLTGENW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCE
FALPKEKLWVTVYETDDEAFDIWANEVGVPRERIIRIGDNKGAPFASDNFWQMGDTGPCG
EECCCCCEEEEEEECCCCHHHHHHHCCCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFFDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRDLIASVAKVTGATDLTNKSLRVIADHIRSCAFLVADGVI
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
PSNENRGYVLRRIIRRAIRHGNMLGAKDTFFWKLVAPLIDVMGSAGDELKQQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELSKLKGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRARESSGFGADYNAMIRVDGASEFKGYDHLELNGKVTALFIDGKAV
CHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCCC
DSVSAGQEAVVILDQTPFYAESGGQVGDKGELKGAGFSFAVSDTQKYGQAIGHIGKVASG
CCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCC
SLKVGDAVQADVDEARRQRIRLNHSATHLMHAALRQVLGTHVAQKGSLVNDKALRFDFSH
CEECCCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEHHH
FEAMKPEEIRAVEDLVNAQIRRNLAIETNIMDIDAARASGAMALFGEKYDDRVRVLRMGD
HHCCCHHHHHHHHHHHHHHHHHCCEEEECEEEEECHHCCCCEEECCCCCCCCEEEEEECC
FSTELCGGTHAARTGDIGLFRITSESGTAAGVRRIEAVTGEGAMAILHAQSDQLNDIAQL
CCHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHH
LKGDSHNLGEKVRAALERTRQLEKELQQLKEQAAAQESANLSSKAEEINGVKLLVSELTG
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHCC
VEPKMLRTMVDDLKNQLGSTIVVLATVADGKVSLIAGVSKDVTDRVKAGELVGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDASALPAALASVKGWVSAKL
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA