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The map label for this gene is yfhA [H]

Identifier: 238896005

GI number: 238896005

Start: 3964226

End: 3965563

Strand: Reverse

Name: yfhA [H]

Synonym: KP1_4133

Alternate gene names: 238896005

Gene position: 3965563-3964226 (Counterclockwise)

Preceding gene: 238896006

Following gene: 238896004

Centisome position: 75.56

GC content: 61.51

Gene sequence:

>1338_bases
ATGACGATCCGTAAACCGGCGCGTTTACTGCTGGTGGATGACGATCCGGGACTGTTGAAGCTACTGGGCATGCGCCTGAT
CAGCGAAGGCTACAGCGTGGTCACCGCCGAGAGCGGGCCGGAAGCGCTGCGCGTGCTGGGGCGTGAGAAAGTGGATCTGG
TGATTAGCGATCTGCGGATGGATGAAATGGACGGCCTGCAGCTGTTTAGCGAGATCCAGAAGGGCCACCCCGGCATGCCG
GTGATCATTCTCACCGCTCACGGTTCGATTCCCGACGCCGTGGCGGCTACCCAGCAGGGGGTGTTCAGCTTCCTGACCAA
GCCGGTCGATAAAGATGCCTTATATAAAGCCATTGATGAGGCGCTGGAGCAGCGCTCGCCGGCCACCGACGAAGCCTGGC
GCCAGGCGATTGTAACCCGCAGCCCGCTGATGCTGCGCCTGCTTGAGCAGGCCGGGATGGTGGCGCAATCGGATGTCAGC
GTACTGATTAATGGCCAGAGCGGGACCGGGAAGGAGATCGTCGCCCAGGCGATCCACAACGCCAGCCCGCGTCATGATAA
GCCGTTCGTGGCGATCAACTGCGGCGCGCTGCCGGAACAGCTGCTCGAATCCGAACTGTTTGGCCATGCGCGGGGCGCTT
TTACCGGCGCGGTCAGTAACCGTGAAGGATTATTCCAGGCCGCTGAGGGTGGTACGCTGTTTCTCGACGAGATCGGTGAT
ATGCCGGTTGCGTTGCAGGTGAAGCTGCTGCGCGTCCTGCAGGAGCGCAAGGTGCGCCCGCTGGGCAGCAACCGCGATAT
CGAAATCAACGTGCGGATTATCTCCGCCACCCACCGCGACCTGCCGAAGGCGATGGCCCGCGGCGAGTTTCGTGAAGACC
TGTTCTACCGCCTCAACGTAGTGAACCTGAAAATACCGCCGCTCTCCGAACGCACGGAAGATATTCCGCTGTTGGCCAAC
CATCTGCTGCGTCAGTCCGCCGATCGCCATAAGCCGTTCGTGCGGGCATTTTCCAGCGATGCGATGAAGCGGCTGATGGC
CGCCAAGTGGCCGGGCAACGTACGCCAGCTGGTGAACGTGATCGAGCAGTGCGTGGCCCTGACTTCGTCGCCAGTGATCG
GCGACGCGCTGGTTGAGCAAGCGCTGGAGGGCGAGAACACGGCGCTGCCGACCTTCGTGGAGGCGCGCAATCAGTTTGAG
CTGAACTACCTGCGCAAGCTGCTGCAAATTACCAAGGGCAACGTCACTCACGCCGCGCGGATGGCAGGGCGCAACCGCAC
CGAATTCTATAAGCTGCTGTCGCGCCATGAGCTCGACGCCAACGACTTTAAAGAGTAG

Upstream 100 bases:

>100_bases
ACCACCCGCAAGCTGGAGAACCTGACGGATATCGAACGCCAGCTCTCCAGCCGCAAGCCGGGCGGCAATTTCGGCAGCGA
AAAAGTGCCGGAGGTGATCC

Downstream 100 bases:

>100_bases
CTCCGCACTATAGCGCAAGTTATGATACGGTAAGCAACCGGTTACGCGATTAAGACAGGAACCCCATGAAAAAGATTGAT
GCGATTATTAAACCTTTCAA

Product: putative 2-component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTIRKPARLLLVDDDPGLLKLLGMRLISEGYSVVTAESGPEALRVLGREKVDLVISDLRMDEMDGLQLFSEIQKGHPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYKAIDEALEQRSPATDEAWRQAIVTRSPLMLRLLEQAGMVAQSDVS
VLINGQSGTGKEIVAQAIHNASPRHDKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
MPVALQVKLLRVLQERKVRPLGSNRDIEINVRIISATHRDLPKAMARGEFREDLFYRLNVVNLKIPPLSERTEDIPLLAN
HLLRQSADRHKPFVRAFSSDAMKRLMAAKWPGNVRQLVNVIEQCVALTSSPVIGDALVEQALEGENTALPTFVEARNQFE
LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Sequences:

>Translated_445_residues
MTIRKPARLLLVDDDPGLLKLLGMRLISEGYSVVTAESGPEALRVLGREKVDLVISDLRMDEMDGLQLFSEIQKGHPGMP
VIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYKAIDEALEQRSPATDEAWRQAIVTRSPLMLRLLEQAGMVAQSDVS
VLINGQSGTGKEIVAQAIHNASPRHDKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
MPVALQVKLLRVLQERKVRPLGSNRDIEINVRIISATHRDLPKAMARGEFREDLFYRLNVVNLKIPPLSERTEDIPLLAN
HLLRQSADRHKPFVRAFSSDAMKRLMAAKWPGNVRQLVNVIEQCVALTSSPVIGDALVEQALEGENTALPTFVEARNQFE
LNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE
>Mature_444_residues
TIRKPARLLLVDDDPGLLKLLGMRLISEGYSVVTAESGPEALRVLGREKVDLVISDLRMDEMDGLQLFSEIQKGHPGMPV
IILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYKAIDEALEQRSPATDEAWRQAIVTRSPLMLRLLEQAGMVAQSDVSV
LINGQSGTGKEIVAQAIHNASPRHDKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGDM
PVALQVKLLRVLQERKVRPLGSNRDIEINVRIISATHRDLPKAMARGEFREDLFYRLNVVNLKIPPLSERTEDIPLLANH
LLRQSADRHKPFVRAFSSDAMKRLMAAKWPGNVRQLVNVIEQCVALTSSPVIGDALVEQALEGENTALPTFVEARNQFEL
NYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE

Specific function: Probable member of a two-component regulatory system yfhA/yfhK [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=441, Percent_Identity=92.0634920634921, Blast_Score=812, Evalue=0.0,
Organism=Escherichia coli, GI1790437, Length=439, Percent_Identity=44.6469248291572, Blast_Score=354, Evalue=5e-99,
Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=40.6593406593407, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1790299, Length=464, Percent_Identity=39.4396551724138, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082117, Length=232, Percent_Identity=56.4655172413793, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1789087, Length=307, Percent_Identity=46.9055374592834, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1789233, Length=250, Percent_Identity=48.8, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=41.4860681114551, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1786524, Length=377, Percent_Identity=39.2572944297082, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI87081872, Length=311, Percent_Identity=39.2282958199357, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787583, Length=238, Percent_Identity=41.1764705882353, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1789828, Length=275, Percent_Identity=35.2727272727273, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=32.8025477707006, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI145693157, Length=110, Percent_Identity=36.3636363636364, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49251; Mature: 49120

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRKPARLLLVDDDPGLLKLLGMRLISEGYSVVTAESGPEALRVLGREKVDLVISDLRM
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCHHHHHHHHHHCC
DEMDGLQLFSEIQKGHPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYKAIDE
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEQRSPATDEAWRQAIVTRSPLMLRLLEQAGMVAQSDVSVLINGQSGTGKEIVAQAIHN
HHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCEEEEECCCCCHHHHHHHHHHC
ASPRHDKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCEEECCCCEEEEHHHCC
MPVALQVKLLRVLQERKVRPLGSNRDIEINVRIISATHRDLPKAMARGEFREDLFYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEE
VNLKIPPLSERTEDIPLLANHLLRQSADRHKPFVRAFSSDAMKRLMAAKWPGNVRQLVNV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IEQCVALTSSPVIGDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAAR
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
TIRKPARLLLVDDDPGLLKLLGMRLISEGYSVVTAESGPEALRVLGREKVDLVISDLRM
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCHHHHHHHHHHCC
DEMDGLQLFSEIQKGHPGMPVIILTAHGSIPDAVAATQQGVFSFLTKPVDKDALYKAIDE
CHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ALEQRSPATDEAWRQAIVTRSPLMLRLLEQAGMVAQSDVSVLINGQSGTGKEIVAQAIHN
HHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEECCCEEEEECCCCCHHHHHHHHHHC
ASPRHDKPFVAINCGALPEQLLESELFGHARGAFTGAVSNREGLFQAAEGGTLFLDEIGD
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCHHCCCCCCCCCEEECCCCEEEEHHHCC
MPVALQVKLLRVLQERKVRPLGSNRDIEINVRIISATHRDLPKAMARGEFREDLFYRLNV
CCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEECHHHHHHHHHHCCCHHHHHHHEEEE
VNLKIPPLSERTEDIPLLANHLLRQSADRHKPFVRAFSSDAMKRLMAAKWPGNVRQLVNV
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
IEQCVALTSSPVIGDALVEQALEGENTALPTFVEARNQFELNYLRKLLQITKGNVTHAAR
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCCHHHHHH
MAGRNRTEFYKLLSRHELDANDFKE
HHCCCHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]