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The map label for this gene is yfhQ [H]

Identifier: 238895989

GI number: 238895989

Start: 3946636

End: 3947370

Strand: Reverse

Name: yfhQ [H]

Synonym: KP1_4116

Alternate gene names: 238895989

Gene position: 3947370-3946636 (Counterclockwise)

Preceding gene: 238895991

Following gene: 238895988

Centisome position: 75.21

GC content: 61.22

Gene sequence:

>735_bases
ATGCTGCAAAATATTCGAATCGTACTGGTAGAAACCTCCCATACCGGCAACATGGGCTCCGTAGCCCGCGCGATGAAAAC
CATGGGCTTAACTAACCTGTGGCTGGTCAATCCGCTGGTCAAACCCGATTCGCAGGCGATAGCCCTGGCCGCGGGCGCCA
GCGACGTGATCGGCAACGCGCAGATTGTCGATACCCTTGATGAAGCGCTGGCAGGCTGTAGCCTGGTGGTTGGCACCAGC
GCGCGCTCTCGCACGCTGCCGTGGCCGATGCTCGACCCGCGCGAATGCGGTCTGAAAAGCGTCGCCGAAGGTCAGCATGC
GCCGGTGGCGCTGGTCTTTGGCCGCGAGCGCGTTGGTCTGACCAACGATGAACTGCAGAAGTGCCACTATCACGTGGCGA
TCGCCGCGAACCCGGAGTACAGCTCGCTGAACCTGGCGATGGCGGTGCAGGTTATCGCCTATGAAGTGCGGATGGCCTGG
CTGGCGGCGCAGGAGCAAGCGCAGCCGGCGGTTGAGCATGAAGAGGCGCCGTACCCGCTGGTGGACGATCTGGAGCGATT
CTACGACCATCTTGAGCAGACGCTGCTGGCGACGGGCTTTATCCGCCCAAATCATCCGGGGCAGGTGATGAACAAGCTGC
GTCGTCTGTTCACCCGCGCGCGTCCGGAAAGCCAGGAGTTGAACATCCTGCGCGGCATGCTGGCGTCTATTGAGCAGCAG
AATAAAGGTAAGTAA

Upstream 100 bases:

>100_bases
TAAAGAACGGGAAAAAACGGCGCATATAATAGCAGCCAAATCTGCTTATGTCTCCGTTTTATGGTAGCATGCACGAATAA
TCTTTAGCCGAAGAACCATA

Downstream 100 bases:

>100_bases
CCGCGTTAACGCCGGATGGCGCTTCGCTTATCCGGGCTACGAATCGGGCTCGCCTGGCGGGATGAAGGTATCAGGCTATG
CGGAAGTAAAACGGCACGGA

Product: hypothetical tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEGQHAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LAAQEQAQPAVEHEEAPYPLVDDLERFYDHLEQTLLATGFIRPNHPGQVMNKLRRLFTRARPESQELNILRGMLASIEQQ
NKGK

Sequences:

>Translated_244_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEGQHAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LAAQEQAQPAVEHEEAPYPLVDDLERFYDHLEQTLLATGFIRPNHPGQVMNKLRRLFTRARPESQELNILRGMLASIEQQ
NKGK
>Mature_244_residues
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNAQIVDTLDEALAGCSLVVGTS
ARSRTLPWPMLDPRECGLKSVAEGQHAPVALVFGRERVGLTNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
LAAQEQAQPAVEHEEAPYPLVDDLERFYDHLEQTLLATGFIRPNHPGQVMNKLRRLFTRARPESQELNILRGMLASIEQQ
NKGK

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=93.0327868852459, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1790865, Length=153, Percent_Identity=36.6013071895425, Blast_Score=85, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26798; Mature: 26798

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHHCCH
QIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEGQHAPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHCCCCCCEEEEECCCCCCC
TNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLAAQEQAQPAVEHEEAPYPL
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
VDDLERFYDHLEQTLLATGFIRPNHPGQVMNKLRRLFTRARPESQELNILRGMLASIEQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NKGK
CCCC
>Mature Secondary Structure
MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDSQAIALAAGASDVIGNA
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCEEEEECCCHHHHCCH
QIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVAEGQHAPVALVFGRERVGL
HHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCHHHCCHHHHHCCCCCCEEEEECCCCCCC
TNDELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAWLAAQEQAQPAVEHEEAPYPL
CHHHHHHCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
VDDLERFYDHLEQTLLATGFIRPNHPGQVMNKLRRLFTRARPESQELNILRGMLASIEQQ
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NKGK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA