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The map label for this gene is ligA [H]

Identifier: 238895883

GI number: 238895883

Start: 3830327

End: 3832342

Strand: Reverse

Name: ligA [H]

Synonym: KP1_4005

Alternate gene names: 238895883

Gene position: 3832342-3830327 (Counterclockwise)

Preceding gene: 238895884

Following gene: 238895882

Centisome position: 73.02

GC content: 60.71

Gene sequence:

>2016_bases
ATGGAACCAATCGAACAACAACTGACTGAACTGCGAACCACGCTTCGCCATCATGAATATCTGTACCACGTTATGGACGC
GCCGGAAGTGCCGGATGCGGAATATGACCGCCTGATGCGCAAGCTGCGCGAGCTGGAGAGCCAGCACCCGGAGCTGATTA
CCCCGGATTCGCCAACTCAGAGGGTGGGGGCGGCGCCACTCACCGCCTTTAGCCAGATCCGCCATGAAGTGCCGATGCTG
TCGCTGGATAACGTGTTTGACGAAGAGAGCTTTCTCGCCTTCAACAAGCGGGTACAGGATCGCCTGAAAAGCACAGATCA
TCTTACCTACTGCTGCGAGCTTAAGCTCGACGGCCTCGCGGTGAGCATTCTCTATGAAAACGGCGTGTTGGTGCAGGCGG
CAACCCGCGGCGACGGCACCACCGGCGAAGATATTACCTCTAATGTGCGAACTATTCGCGCTATCCCCCTCAAGCTGCAT
GGCGAGAATATTCCTGCACGCCTTGAGGTACGTGGGGAAGTGTTCCTCCCGCAGGCCGGGTTTGAAAAAATCAATGAGGA
AGCTCGCCGCACCGGCGGGAAAGTGTTTGCTAACCCGCGTAATGCGGCAGCGGGTTCGCTGCGCCAGCTCGATCCGCGCA
TCACTGCGAAGCGACCGCTTACTTTCTTCTGCTACGGCGTGGGCGTGCTGGAGGGCGGCGAGCTGCCGGCCAGCCACTCG
GCTCGGTTGCTGCAGTTCAAAGCATGGGGGCTGCCGGTGAGCGATCGCGTCACCCTGTGCCATACCCCCGAGGAAGTGCT
TACTTACTATCGTAAAGTCGAGGAAGAGCGTCCCCACCTGGGCTTTGACATTGATGGCGTGGTGATCAAAGTCGATTCGC
TGGCGCTGCAGGAACAGCTGGGTTTCGTCGCCCGCGCGCCGCGCTGGGCCGTGGCCTTTAAGTTCCCGGCACAGGAGCAG
ATGACCTTCGTCCGGGACGTGGAGTTCCAGGTGGGCCGCACCGGGGCGATTACGCCGGTGGCGCGTCTGGAGCCGGTCCA
TGTTGCCGGCGTGTTGGTGAGCAATGCCACCCTGCATAATGCCGATGAAATTGAACGCTTAGGCTTGAAAATCGGCGACA
AGGTGGTGATTCGCCGGGCAGGCGATGTGATCCCGCAGGTGGTTAACGTGGTGCTTTCCGAGCGTCCGGCGGATGCCCGG
GATGTCGTCTTCCCGACCCACTGTCCTGTATGTCAGTCAGACGTTGAACGGGTGGAAGGCGAGGCGGTAGCCCGCTGTAC
GGGCGGCCTGATCTGCGGCGCGCAGCGCAAAGAGTCGCTCAAGCACTTCGTCTCCCGTCGCGCGCTCGACGTTGACGGCA
TGGGCGACAAGATCATCGACCAGCTGGTGGAGAAAGAGTACGTCCATACCCCGGCGGATCTGTTCCGGCTGACGGCGGGG
AAACTGACCGGGCTGGAGCGGATGGGGCCGAAGTCCGCGCAAAACGTAGTCAACGCCCTCGAGAAAGCCAAAGAGACGAC
CTTCGCACGTTTCCTCTACGCCTTAGGTATTCGCGAGGTAGGAGAAGCGACGGCGGCGGGCCTGGCGGCGCATTTCGGCA
CTCTCGAAGCCCTTGAGCAGGCTACCATTGAGGAGCTACAGAAAGTCCCTGATGTCGGCATCGTCGTCGCTACCCATACC
TTCAACTTCTTTGCCGAAGAGAGCAACCGCGATGTCATTGCGCAACTGCTGGCGGAGGGGGTTCGCTGGCCTGCGCCGGT
GGTGGTGAAGGCGGAAGAGATTGATAGCCCGTTTGCAGGCAAAACCGTGGTCCTTACCGGCAGCCTCAGCCAGCTGTCGC
GCGATGACGCCAAAGAGCGTCTGGTGGCGCTGGGGGCGAAGGTGGCAGGCAGCGTGTCGAAGAAAACCGACCTGGTGATT
GCCGGCGAAGCGGCGGGGTCTAAGCTGGCGAAAGCGCAGGAGCTGGGCATCGAGGTGATTGACGAAGCCGAAATGATGCG
TCTGCTGGGAGAATAA

Upstream 100 bases:

>100_bases
TATTCGCGAAGTGAAAGACGCGAACGCCTGACGTTCCGCGTTAACTCAATTCCTTCTCAACCCCCGCCTGTCGGGGGTTT
TTTATCATTGATGGTGTGAT

Downstream 100 bases:

>100_bases
CGTGGATAAAGCGCAGCTGGTCGAGATAGCCAATACCGAAATGCCGTTCGGTAAATACAAGGGACGTCGCTTAATCGATG
TCCCGGAAGAGTATTTATTG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRKLRELESQHPELITPDSPTQRVGAAPLTAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTDHLTYCCELKLDGLAVSILYENGVLVQAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEEARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPASHS
ARLLQFKAWGLPVSDRVTLCHTPEEVLTYYRKVEEERPHLGFDIDGVVIKVDSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLKIGDKVVIRRAGDVIPQVVNVVLSERPADAR
DVVFPTHCPVCQSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRALDVDGMGDKIIDQLVEKEYVHTPADLFRLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAHFGTLEALEQATIEELQKVPDVGIVVATHT
FNFFAEESNRDVIAQLLAEGVRWPAPVVVKAEEIDSPFAGKTVVLTGSLSQLSRDDAKERLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMMRLLGE

Sequences:

>Translated_671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRKLRELESQHPELITPDSPTQRVGAAPLTAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTDHLTYCCELKLDGLAVSILYENGVLVQAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEEARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPASHS
ARLLQFKAWGLPVSDRVTLCHTPEEVLTYYRKVEEERPHLGFDIDGVVIKVDSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLKIGDKVVIRRAGDVIPQVVNVVLSERPADAR
DVVFPTHCPVCQSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRALDVDGMGDKIIDQLVEKEYVHTPADLFRLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAHFGTLEALEQATIEELQKVPDVGIVVATHT
FNFFAEESNRDVIAQLLAEGVRWPAPVVVKAEEIDSPFAGKTVVLTGSLSQLSRDDAKERLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMMRLLGE
>Mature_671_residues
MEPIEQQLTELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRKLRELESQHPELITPDSPTQRVGAAPLTAFSQIRHEVPML
SLDNVFDEESFLAFNKRVQDRLKSTDHLTYCCELKLDGLAVSILYENGVLVQAATRGDGTTGEDITSNVRTIRAIPLKLH
GENIPARLEVRGEVFLPQAGFEKINEEARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPASHS
ARLLQFKAWGLPVSDRVTLCHTPEEVLTYYRKVEEERPHLGFDIDGVVIKVDSLALQEQLGFVARAPRWAVAFKFPAQEQ
MTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHNADEIERLGLKIGDKVVIRRAGDVIPQVVNVVLSERPADAR
DVVFPTHCPVCQSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRALDVDGMGDKIIDQLVEKEYVHTPADLFRLTAG
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAHFGTLEALEQATIEELQKVPDVGIVVATHT
FNFFAEESNRDVIAQLLAEGVRWPAPVVVKAEEIDSPFAGKTVVLTGSLSQLSRDDAKERLVALGAKVAGSVSKKTDLVI
AGEAAGSKLAKAQELGIEVIDEAEMMRLLGE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=670, Percent_Identity=92.089552238806, Blast_Score=1266, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=524, Percent_Identity=23.2824427480916, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73694; Mature: 73694

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS50172 BRCT ; PS00435 PEROXIDASE_1 ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIEQQLTELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRKLRELESQHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLTAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTDHLTYCCELKLDGLA
HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVQAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCHH
FEKINEEARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPASHS
HHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC
ARLLQFKAWGLPVSDRVTLCHTPEEVLTYYRKVEEERPHLGFDIDGVVIKVDSLALQEQL
CEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHH
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLKIGDKVVIRRAGDVIPQVVNVVLSERPADARDVVFPTHCPVCQSDVERVEG
HHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRALDVDGMGDKIIDQLVEKEYVHTPADLFRLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAHFGTLEALEQ
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIEELQKVPDVGIVVATHTFNFFAEESNRDVIAQLLAEGVRWPAPVVVKAEEIDSPFAG
HHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQLSRDDAKERLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCHHC
DEAEMMRLLGE
CHHHHHHHHCC
>Mature Secondary Structure
MEPIEQQLTELRTTLRHHEYLYHVMDAPEVPDAEYDRLMRKLRELESQHPELITPDSPTQ
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEECCCCCHH
RVGAAPLTAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRLKSTDHLTYCCELKLDGLA
HCCCCHHHHHHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEE
VSILYENGVLVQAATRGDGTTGEDITSNVRTIRAIPLKLHGENIPARLEVRGEVFLPQAG
EEEEEECCEEEEEECCCCCCCCHHHHHCCEEEEEEEEEEECCCCCEEEEECCEEECCCHH
FEKINEEARRTGGKVFANPRNAAAGSLRQLDPRITAKRPLTFFCYGVGVLEGGELPASHS
HHHHHHHHHHCCCCEEECCCCCCCCCHHHCCCCCCCCCCCEEEEEEEEEECCCCCCCCCC
ARLLQFKAWGLPVSDRVTLCHTPEEVLTYYRKVEEERPHLGFDIDGVVIKVDSLALQEQL
CEEEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHH
GFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRTGAITPVARLEPVHVAGVLVSNATLHN
HHHHCCCCEEEEEECCCHHHHHHHHHCHHCCCCCCCCCCHHHCCCEEEEEEEEECCCCCC
ADEIERLGLKIGDKVVIRRAGDVIPQVVNVVLSERPADARDVVFPTHCPVCQSDVERVEG
HHHHHHHCCCCCCHHEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCC
EAVARCTGGLICGAQRKESLKHFVSRRALDVDGMGDKIIDQLVEKEYVHTPADLFRLTAG
HHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
KLTGLERMGPKSAQNVVNALEKAKETTFARFLYALGIREVGEATAAGLAAHFGTLEALEQ
HHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATIEELQKVPDVGIVVATHTFNFFAEESNRDVIAQLLAEGVRWPAPVVVKAEEIDSPFAG
HHHHHHHHCCCCEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCC
KTVVLTGSLSQLSRDDAKERLVALGAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCHHC
DEAEMMRLLGE
CHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA