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The map label for this gene is vacJ [H]

Identifier: 238895858

GI number: 238895858

Start: 3802099

End: 3802860

Strand: Reverse

Name: vacJ [H]

Synonym: KP1_3977

Alternate gene names: 238895858

Gene position: 3802860-3802099 (Counterclockwise)

Preceding gene: 238895862

Following gene: 238895856

Centisome position: 72.46

GC content: 56.82

Gene sequence:

>762_bases
ATGAATTATCGCCTGTCGGCGCTTGCGCTTGGCGCCACATTGCTGGTGGGATGTGCCAGTTCCAGCTCTGGCGATCGGCC
GCAGGGGCGCTCAGACCCGTTAGAAGGGTTTAACCGCACGATGTTTAACTTCAACTTTAACGTGGTTGATCCTTACGTGC
TTCGCCCCGTCGCGGTGGCATGGCGCGACTACGTCCCACAGCCGGCGCGCAACGGGTTAAGCAATTTTACCAGTAACCTG
GAAGAGCCGGCAGTGATGGTGAACTACTTCCTGCAGGGCGACCCCTATAAAGGTATGGTGCATTTTACCCGCTTCTTCCT
GAACACCATTCTCGGTATGGGTGGGCTTATCGATGTGGCCGGGATGGCGAATCCGCAGCTACAGCGGGTTGAGCCACATC
GTTTCGGCAGTACGCTTGGGCATTATGGCGTTGGCTATGGCCCGTATGTTCAGTTGCCGTTCTATGGAAGCTTTACCCTG
CGCGACGAAGGCGGCGATATGGCCGATGGCTTGTATCCGGTGCTCTCCTGGCTGACCTGGCCGATGTCGATCGGTAAATG
GGCGGTGGAAGGGATTGAGACACGTGCTCAGCTGCTGGATTCCGACGGCCTGCTGCGCCAGTCTTCCGACCCTTACATTC
TGATGCGTGAAGCCTACTTCCAGCGTCATGACTTTATCGCCAACGGCGGTAAGCTGACCCCGGCGGACAACCCGAATGCT
CAGGCTATTCAGGATGAGCTGAAGGATATCGATTCACAGTGA

Upstream 100 bases:

>100_bases
CAACGTGCTGTTACAATCGCTCACATCTTTACGGGCGGAAGCGTTGTCTTTCCGTCATGGATGGCAACTCGCGATAGCCA
TAAAAACCAGGGAGACATCT

Downstream 100 bases:

>100_bases
TCGGCGAATGATAAAAAAAGGGTGAACATAATGTTCACCCTTTTTTGTGGCGCGCTGCTTATCAGAAGCGGTAGTTGAAG
TTAGCGCCGTACAGCCAGGC

Product: lipoprotein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNYRLSALALGATLLVGCASSSSGDRPQGRSDPLEGFNRTMFNFNFNVVDPYVLRPVAVAWRDYVPQPARNGLSNFTSNL
EEPAVMVNYFLQGDPYKGMVHFTRFFLNTILGMGGLIDVAGMANPQLQRVEPHRFGSTLGHYGVGYGPYVQLPFYGSFTL
RDEGGDMADGLYPVLSWLTWPMSIGKWAVEGIETRAQLLDSDGLLRQSSDPYILMREAYFQRHDFIANGGKLTPADNPNA
QAIQDELKDIDSQ

Sequences:

>Translated_253_residues
MNYRLSALALGATLLVGCASSSSGDRPQGRSDPLEGFNRTMFNFNFNVVDPYVLRPVAVAWRDYVPQPARNGLSNFTSNL
EEPAVMVNYFLQGDPYKGMVHFTRFFLNTILGMGGLIDVAGMANPQLQRVEPHRFGSTLGHYGVGYGPYVQLPFYGSFTL
RDEGGDMADGLYPVLSWLTWPMSIGKWAVEGIETRAQLLDSDGLLRQSSDPYILMREAYFQRHDFIANGGKLTPADNPNA
QAIQDELKDIDSQ
>Mature_253_residues
MNYRLSALALGATLLVGCASSSSGDRPQGRSDPLEGFNRTMFNFNFNVVDPYVLRPVAVAWRDYVPQPARNGLSNFTSNL
EEPAVMVNYFLQGDPYKGMVHFTRFFLNTILGMGGLIDVAGMANPQLQRVEPHRFGSTLGHYGVGYGPYVQLPFYGSFTL
RDEGGDMADGLYPVLSWLTWPMSIGKWAVEGIETRAQLLDSDGLLRQSSDPYILMREAYFQRHDFIANGGKLTPADNPNA
QAIQDELKDIDSQ

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=253, Percent_Identity=88.1422924901186, Blast_Score=468, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28088; Mature: 28088

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYRLSALALGATLLVGCASSSSGDRPQGRSDPLEGFNRTMFNFNFNVVDPYVLRPVAVA
CCEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHCCEEEEECCCEECHHHHHHHHHH
WRDYVPQPARNGLSNFTSNLEEPAVMVNYFLQGDPYKGMVHFTRFFLNTILGMGGLIDVA
HHHHCCCHHHHHHHHHHHCCCCCCEEEHHEECCCCCCHHHHHHHHHHHHHHCCCCCEECC
GMANPQLQRVEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDEGGDMADGLYPVLSWLTW
CCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
PMSIGKWAVEGIETRAQLLDSDGLLRQSSDPYILMREAYFQRHDFIANGGKLTPADNPNA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEECCCCCCCH
QAIQDELKDIDSQ
HHHHHHHHHCCCC
>Mature Secondary Structure
MNYRLSALALGATLLVGCASSSSGDRPQGRSDPLEGFNRTMFNFNFNVVDPYVLRPVAVA
CCEEHHHHHHHHHHHHEECCCCCCCCCCCCCCCHHHHCCEEEEECCCEECHHHHHHHHHH
WRDYVPQPARNGLSNFTSNLEEPAVMVNYFLQGDPYKGMVHFTRFFLNTILGMGGLIDVA
HHHHCCCHHHHHHHHHHHCCCCCCEEEHHEECCCCCCHHHHHHHHHHHHHHCCCCCEECC
GMANPQLQRVEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRDEGGDMADGLYPVLSWLTW
CCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHH
PMSIGKWAVEGIETRAQLLDSDGLLRQSSDPYILMREAYFQRHDFIANGGKLTPADNPNA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCEECCCCCCCH
QAIQDELKDIDSQ
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]