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The map label for this gene is mepA [H]

Identifier: 238895849

GI number: 238895849

Start: 3792122

End: 3792946

Strand: Reverse

Name: mepA [H]

Synonym: KP1_3968

Alternate gene names: 238895849

Gene position: 3792946-3792122 (Counterclockwise)

Preceding gene: 238895850

Following gene: 238895848

Centisome position: 72.27

GC content: 62.42

Gene sequence:

>825_bases
ATGAAAAACACCGTTATCGCACTGCTTGCGCTGTTGGCTAGCGCCGGTAGCCTGGCCGCCACGCCATGGCAGAAAATAAC
CCAACCGATCGACGGCAGCGCCCAGTCAATCGGCGCGTTTTCCAATGGCTGTATCGTTGGCGCCGAGGCGCTCCCCCTGA
GTGCCCCCGGCTATCAGGTGATGCGTACCGATCAGCGTCGCTACTTCGGTCATCCGGATCTGGTTCAGTTTATTCAGCGC
CTGAGTAATCAGGTGCACAATAAAGGTATGGGAACCGTGCTGATTGGCGACATGGGGATGCCGGCGGGCGGGCGCTTTAA
CGGCGGGCACGCCAGCCACCAGACGGGCCTCGATGTCGATATCTTCCTTCAGCTGCCGCAAACGCGCTGGACATCGTCCC
AGCTGTTGAAGCCGCAGGCGCTGGACCTGGTCGCCAGCGATGGCAAGCATGTGGTGCCGTCGCTATGGAGCCCGCAAATC
AGCCAGCTTATCAAGCTGGCGGCGGAAGATAGCGAAGTTACCCGCATCTTCGTCAACCCGGCGATTAAACAGCAGCTGTG
CCTGGACGCGGGCAGCGACCGCCAGTGGCTGCGTAAAGTCCGTCCCTGGTTCCAGCATCGCGCTCATATGCACGTCCGAC
TGCGTTGCCCGGCGGGAAGTCTGGAGTGCGAGGATCAGGCGCCGCCGCCGCCCGGCGATGGCTGCGGAGCGGAGCTGCAG
AGCTGGTTCGAACCACCTAAACCCGGGTCTACCCCCCCTGTGAAGAAGACGCCGCCGCCGCTGCCGCCTTCCTGCCAGGC
GCTACTGGATGAGCATGTGCTGTAA

Upstream 100 bases:

>100_bases
CGATCGCTGAGGCGATGCTGGCGATCGTTCTGATGGATCACTTTATGCGTCAGCGCGCGCAGAATGGCGACGTGACGACG
ACAATTCCACGCTGGTAACC

Downstream 100 bases:

>100_bases
TGGATAGTTTTATCGAGTTGTTTGCCGTATCGCCGCTGGTGCTGGTGGTACTCTTCTTTGTCGCCATCCTGGCCGGTTTT
ATTGACTCACTGGCGGGAGG

Product: penicillin-insensitive murein endopeptidase

Products: Split Peptidoglycan Strands [C]

Alternate protein names: D-alanyl-D-alanine-endopeptidase; DD-endopeptidase [H]

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MKNTVIALLALLASAGSLAATPWQKITQPIDGSAQSIGAFSNGCIVGAEALPLSAPGYQVMRTDQRRYFGHPDLVQFIQR
LSNQVHNKGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPQTRWTSSQLLKPQALDLVASDGKHVVPSLWSPQI
SQLIKLAAEDSEVTRIFVNPAIKQQLCLDAGSDRQWLRKVRPWFQHRAHMHVRLRCPAGSLECEDQAPPPPGDGCGAELQ
SWFEPPKPGSTPPVKKTPPPLPPSCQALLDEHVL

Sequences:

>Translated_274_residues
MKNTVIALLALLASAGSLAATPWQKITQPIDGSAQSIGAFSNGCIVGAEALPLSAPGYQVMRTDQRRYFGHPDLVQFIQR
LSNQVHNKGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPQTRWTSSQLLKPQALDLVASDGKHVVPSLWSPQI
SQLIKLAAEDSEVTRIFVNPAIKQQLCLDAGSDRQWLRKVRPWFQHRAHMHVRLRCPAGSLECEDQAPPPPGDGCGAELQ
SWFEPPKPGSTPPVKKTPPPLPPSCQALLDEHVL
>Mature_274_residues
MKNTVIALLALLASAGSLAATPWQKITQPIDGSAQSIGAFSNGCIVGAEALPLSAPGYQVMRTDQRRYFGHPDLVQFIQR
LSNQVHNKGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVDIFLQLPQTRWTSSQLLKPQALDLVASDGKHVVPSLWSPQI
SQLIKLAAEDSEVTRIFVNPAIKQQLCLDAGSDRQWLRKVRPWFQHRAHMHVRLRCPAGSLECEDQAPPPPGDGCGAELQ
SWFEPPKPGSTPPVKKTPPPLPPSCQALLDEHVL

Specific function: Involved in the removal of murein from the sacculus. May also facilitate integration of nascent murein strands into the sacculus by cleaving the peptide bonds between neighboring strands in mature murein [H]

COG id: COG3770

COG function: function code M; Murein endopeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M74 family [H]

Homologues:

Organism=Escherichia coli, GI1788668, Length=274, Percent_Identity=84.3065693430657, Blast_Score=459, Evalue=1e-131,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR005073 [H]

Pfam domain/function: PF03411 Peptidase_M74 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 29700; Mature: 29700

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTVIALLALLASAGSLAATPWQKITQPIDGSAQSIGAFSNGCIVGAEALPLSAPGYQV
CCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCEEECCCCCCCCCCCHHH
MRTDQRRYFGHPDLVQFIQRLSNQVHNKGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
HCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IFLQLPQTRWTSSQLLKPQALDLVASDGKHVVPSLWSPQISQLIKLAAEDSEVTRIFVNP
EEEECCCCCCCHHHHCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEECH
AIKQQLCLDAGSDRQWLRKVRPWFQHRAHMHVRLRCPAGSLECEDQAPPPPGDGCGAELQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
SWFEPPKPGSTPPVKKTPPPLPPSCQALLDEHVL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKNTVIALLALLASAGSLAATPWQKITQPIDGSAQSIGAFSNGCIVGAEALPLSAPGYQV
CCHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCEEECCCCCCCCCCCHHH
MRTDQRRYFGHPDLVQFIQRLSNQVHNKGMGTVLIGDMGMPAGGRFNGGHASHQTGLDVD
HCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEE
IFLQLPQTRWTSSQLLKPQALDLVASDGKHVVPSLWSPQISQLIKLAAEDSEVTRIFVNP
EEEECCCCCCCHHHHCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHCCCCCEEEEEECH
AIKQQLCLDAGSDRQWLRKVRPWFQHRAHMHVRLRCPAGSLECEDQAPPPPGDGCGAELQ
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
SWFEPPKPGSTPPVKKTPPPLPPSCQALLDEHVL
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond [C]

Specific reaction: Split The D-Alanyl-Gamma-Meso-2,6-Diamino- Pimelyl Peptide Bond Connecting Neighboring Peptidoglycan Strands. [C]

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Endopeptidases of unknown catalytic mechanism [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA