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The map label for this gene is folC [H]

Identifier: 238895835

GI number: 238895835

Start: 3778302

End: 3779570

Strand: Reverse

Name: folC [H]

Synonym: KP1_3953

Alternate gene names: 238895835

Gene position: 3779570-3778302 (Counterclockwise)

Preceding gene: 238895836

Following gene: 238895834

Centisome position: 72.01

GC content: 62.81

Gene sequence:

>1269_bases
ATGGAAAAAGATCCTATTCCTCAGGCCACGTCGCCCCTGGCTACGTGGCTTTCTTATCTGGAACATCTGCACAGTAAAAC
CATCGACCTTGGGCTGGCGCGCGTCAGCGAGGTCGCCGGGCAGATGGATATCGTTAAACCGGCGCCGTTCGTTTTTACCG
TCGCGGGCACCAATGGCAAAGGCACCACCTGCCGCACGCTGGAAACCATCCTGATGGCGGCCGGTTATAAAGTGGGGGTC
TACAGTTCTCCGCATCTGGTGCGCTACACCGAACGCGTGCGCATTCAGGGGGCTGAACTGCCGGAGGCGGCGCATACGGC
TTCGTTTGCTGAAATCGAAGCGGCGCGGGGTGACATCTCGCTGACCTATTTTGAATACGGCACGCTCTCGGCGCTGTGGC
TGTTTAAGCAGGCGCAGCTGGATGTGGTGATCCTTGAGGTCGGGCTCGGCGGGCGTCTCGACGCCACCAACATCGTGGAT
GCCGACGTCGCGGTGGTCACCAGCATCGCCCTCGATCATACCGACTGGCTGGGGCCGGATCGCGAAAGTATCGGCCGGGA
AAAAGCGGGTATCTTTCGCGCCGGTAAACCGGCGGTGGTGGGGGAGCCGGATATGCCGCTCACCATCGCGGAAGTGGCTA
GCGAGAAGGGCGCGCTGCTCCAGCGCCGCGGCGTCGACTGGCGCTATGAGGTGGAAGGCGAAACATGGTCCTTCCGCGAT
ACGGCAGGGGCGTTAAGCCGCCTGCCTCTGCCTCAGGTGCCGTTGCCGAATGCGGCGACGGCCGTGGCGGCGCTGCGCGC
CAGCGGGCTTGCCGTGGATGACGCTATCCTGCGCGCAGGGATCCGCGATGCGATGCTCCCGGGACGCTTTCAGATAATCA
GCGACGCCCCGCGAGTGATCCTCGATGTGGCGCACAACCCGCACGCGGCGGCCTATCTCGCCGGGCGTCTCAAAACGTTA
GCGAAAACGGGACGCGTGCTGGCGGTTATTGGTATGCTGCATGATAAGGATATCGCCGGCACGTTGGCCAATCTGGCCCC
GGAAGTCGATGCCTGGTACTGCGCGCCGCTGGAAGGCCCGCGCGGCGCGACGGCAGAGCAGCTGGTGGAACATTTGCGCT
GCGGTACAGTCTATAGCAGCGTGGCGCAGGCGTGGCGTGCGGCCATGGCTGACGCTAAAGTGGAAGATACCGTGCTGGTG
TGTGGTTCGTTTCACACCGTAGCGCAGGTTATGGAAGAGATAGACGCGGGGAGAATCGGTGGCGAGTAA

Upstream 100 bases:

>100_bases
ACCGGATCAGGAACCTGAGGCCTGATAAAGCAGAGGGCAGGGCCTGGCGGCGCTGCCCTTTTTTATTCATACGCGTTAAC
CGTCAATTCTTACGGGCATC

Downstream 100 bases:

>100_bases
GTTTCAGAACCGTTTAGTCGGGACAATCGTGCTGGTGGCGCTGGGGGTGATTATCCTGCCAGGGCTGCTGGACGGGCAGA
AAAAGCATTACCAGGATGAG

Product: bifunctional folylpolyglutamate synthase/ dihydrofolate synthase

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGKGTTCRTLETILMAAGYKVGV
YSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRD
TAGALSRLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVILDVAHNPHAAAYLAGRLKTL
AKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGPRGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLV
CGSFHTVAQVMEEIDAGRIGGE

Sequences:

>Translated_422_residues
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGKGTTCRTLETILMAAGYKVGV
YSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRD
TAGALSRLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVILDVAHNPHAAAYLAGRLKTL
AKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGPRGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLV
CGSFHTVAQVMEEIDAGRIGGE
>Mature_422_residues
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGKGTTCRTLETILMAAGYKVGV
YSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRD
TAGALSRLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVILDVAHNPHAAAYLAGRLKTL
AKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGPRGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLV
CGSFHTVAQVMEEIDAGRIGGE

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=193, Percent_Identity=37.8238341968912, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI66932990, Length=193, Percent_Identity=37.8238341968912, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1788654, Length=416, Percent_Identity=81.0096153846154, Blast_Score=668, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17553148, Length=439, Percent_Identity=27.5626423690205, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=439, Percent_Identity=27.5626423690205, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=439, Percent_Identity=27.5626423690205, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=346, Percent_Identity=28.6127167630058, Blast_Score=123, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324815, Length=380, Percent_Identity=26.0526315789474, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24641571, Length=285, Percent_Identity=31.5789473684211, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581568, Length=177, Percent_Identity=36.1581920903955, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45090; Mature: 45090

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCC
GTTCRTLETILMAAGYKVGVYSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDIS
CCHHHHHHHHHHHCCCEEEECCCCCCEEEEHHHEEECCCCCCHHHHHHHHHHHHCCCCEE
LTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPD
EEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
RESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRD
HHHHCCHHHCCEECCCCCEECCCCCCEEHHHHHCCCCHHHHHCCCCEEEEECCCEEECHH
TAGALSRLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVI
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCEEEECCCCEEE
LDVAHNPHAAAYLAGRLKTLAKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGP
EEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHCCCCCCCEEEECCCCC
RGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLVCGSFHTVAQVMEEIDAGRIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCC
GE
CC
>Mature Secondary Structure
MEKDPIPQATSPLATWLSYLEHLHSKTIDLGLARVSEVAGQMDIVKPAPFVFTVAGTNGK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCEEEEEECCCCC
GTTCRTLETILMAAGYKVGVYSSPHLVRYTERVRIQGAELPEAAHTASFAEIEAARGDIS
CCHHHHHHHHHHHCCCEEEECCCCCCEEEEHHHEEECCCCCCHHHHHHHHHHHHCCCCEE
LTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVDADVAVVTSIALDHTDWLGPD
EEEEECCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
RESIGREKAGIFRAGKPAVVGEPDMPLTIAEVASEKGALLQRRGVDWRYEVEGETWSFRD
HHHHCCHHHCCEECCCCCEECCCCCCEEHHHHHCCCCHHHHHCCCCEEEEECCCEEECHH
TAGALSRLPLPQVPLPNAATAVAALRASGLAVDDAILRAGIRDAMLPGRFQIISDAPRVI
HHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCEEEECCCCEEE
LDVAHNPHAAAYLAGRLKTLAKTGRVLAVIGMLHDKDIAGTLANLAPEVDAWYCAPLEGP
EEECCCCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHCCCCCCCEEEECCCCC
RGATAEQLVEHLRCGTVYSSVAQAWRAAMADAKVEDTVLVCGSFHTVAQVMEEIDAGRIG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCC
GE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]