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The map label for this gene is nuoB [H]

Identifier: 238895804

GI number: 238895804

Start: 3752414

End: 3753088

Strand: Reverse

Name: nuoB [H]

Synonym: KP1_3921

Alternate gene names: 238895804

Gene position: 3753088-3752414 (Counterclockwise)

Preceding gene: 238895805

Following gene: 238895803

Centisome position: 71.51

GC content: 55.7

Gene sequence:

>675_bases
ATGGATTATACGCTCACCCGCATAGATCCTAACGGTGAGAACGACCGTTACCCCCTGCAGAAACAGGAGATCGTTACCGA
TCCTCTGGAGCAGGAAGTCAATAAAAGCGTGTATATGGGGAAACTCGAACACGCGCTGCATGACATGGTGAACTGGGGCC
GTAAGAACTCAATTTGGCCGTACAACTTCGGCCTTTCTTGCTGTTATGTGGAAATGGTGACGTCATTCACTGCGGTGCAT
GACGTTGCGCGTTTTGGGGCAGAAGTTCTGCGCGCCTCTCCGCGTCAGGCGGACCTGATGGTGGTCGCGGGAACATGCTT
TACCAAAATGGCGCCGGTTATTCAGCGTCTCTACGATCAGATGCTGGAGCCGAAGTGGGTGATCTCCATGGGCGCCTGCG
CCAACTCAGGCGGGATGTACGACATCTATTCCGTCGTCCAGGGCGTGGATAAATTCATTCCGGTCGATGTGTACATCCCG
GGCTGCCCGCCGCGTCCGGAGGCCTATATGCAGGCGCTGATGCTGCTGCAGGAGTCCATTGGTAAAGAACGTCGTCCGCT
CTCATGGGTGGTTGGCGATCAGGGCGTTTATCGCGCCAATATGCAGTCGGAACGCGAGCGTAAACGCGGCGAACGTATTG
CAGTGACCAATCTGCGGACGCCGGACGAGATTTAA

Upstream 100 bases:

>100_bases
CGCACTGGACTGGACGCCTGCGCGTTCCCGTCGCACGCTGGTTAACCCGGAAACAGACAGTCCCACTAACCGTCATATGC
AGTAAAAGCGAGGCAATAAG

Downstream 100 bases:

>100_bases
TTTGCGCCTGCGGGCGGGAGAACCACTTCGCAAATCAATCTACAACAGCGCGAAGTCTCGCCGGCAGTCACCACGGACCT
TTTGCAATGGTGAACAATAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKSVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI

Sequences:

>Translated_224_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKSVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI
>Mature_224_residues
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKSVYMGKLEHALHDMVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVH
DVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIP
GCPPRPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=145, Percent_Identity=54.4827586206897, Blast_Score=172, Evalue=2e-43,
Organism=Escherichia coli, GI1788624, Length=224, Percent_Identity=96.875, Blast_Score=449, Evalue=1e-128,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=41.8032786885246, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1788834, Length=160, Percent_Identity=33.75, Blast_Score=91, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=153, Percent_Identity=52.2875816993464, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI18859983, Length=145, Percent_Identity=54.4827586206897, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24642371, Length=145, Percent_Identity=54.4827586206897, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24651058, Length=145, Percent_Identity=53.7931034482759, Blast_Score=169, Evalue=1e-42,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 25519; Mature: 25519

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.4 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKSVYMGKLEHALHDMVNWGRKNSIWP
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI
CCCCCCCEEEECCCCEEECCHHHHHHHHCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKSVYMGKLEHALHDMVNWGRKNSIWP
CCCEEEEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
YNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTCFTKMAPVIQRLYDQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHH
MLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPPRPEAYMQALMLLQESI
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHH
GKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPDEI
CCCCCCCEEEECCCCEEECCHHHHHHHHCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA