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The map label for this gene is galS [H]

Identifier: 238895715

GI number: 238895715

Start: 3644288

End: 3645310

Strand: Reverse

Name: galS [H]

Synonym: KP1_3816

Alternate gene names: 238895715

Gene position: 3645310-3644288 (Counterclockwise)

Preceding gene: 238895716

Following gene: 238895714

Centisome position: 69.45

GC content: 61.29

Gene sequence:

>1023_bases
ATGATCACCATTCGTGATGTCGCCCGCCAGGCGGGCGTTTCCGTGGCCACCGTGTCGCGGGTGCTGAATAACAGCGCGCT
GGTGAGTCCGGATACGCGCGACGTGGTGATGAAAGCGGTGACCCAACTGGGCTACCGGCCCAACGCCAACGCCCAGGCGC
TGGCCACCCAGGTCAGCGATACCATCGGCGTGGTGGTGATGGACGTCTCCGACGCCTTCTTCGGCGCGCTGGTGAAAGCA
GTGGATACCGTTGCTCAGCAGCATCAGAAATATGTGCTGATCGGCAACAGCTATCACGAGGCGGAAAAAGAGCGTCACGC
CATCGAGGTGCTGATCCGTCAGCGCTGCTCGGCGCTGATCGTCCATTCGAAAGCGCTGAGCGATGATGAACTGAGCGACT
TTATGCAACATATCCCGGGGATGGTGCTGATCAACCGCATCGTGCCGGGGTATGCCCATCGCTGCGTCGGACTCGACAAC
GTCAGCGGCGCGCTGATGGCCACCCGTATGCTGCTCAATCATGGTCACCAGCGCATCGGCTATCTCTCCTCAAACCACGG
CATTGAAGATGACGATATGCGCCGGGAAGGGTGGAGCAAAGCGCTGCAGGAGCAAGGCATCATCGCCCCGGACAGCTGGA
TCGGTTCCGGCTCCCCGGATATGCAGGGCGGTGAAGCGGCGATGGTCGAACTGCTGGGGCGCAATCTCGGCCTCACCGCG
GTGTTTGCCTATAACGACAGCATGGCGGCCGGCGCGCTGACGACGCTGAAGGACAACGGCATTGTCGTGCCGCAGCATCT
GTCGCTGATTGGCTTTGATGATATTCCTATTTCCCGTTACACCGATCCTCAGCTCACCACCGTGCGCTATCCGGTAATGT
CGATGGCAAAGCTGGCCACCGAGCTGGCGCTGCTGGGGGCGGCCGGCAAGCTGGATCGCGAGGCAACGCACTGCTTTATG
CCGACGCTGGTGCGCCGTCATTCCGTTGCCCAGCGGCAAACTGTGGGGCCGATCACTAACTGA

Upstream 100 bases:

>100_bases
CTCACTCACGCAGCGGACCGCGGGTCGCTGCCAGAATAGCCTCCTTGCTAAGCACAGGAGGTGAATGTGACGTGCTGTAA
TTCTCTTAAGGTCGCTGAGT

Downstream 100 bases:

>100_bases
CGCGATCGCGGGATGTAACCGCTTTCAATTTGTGAGTAAATTCACAGTATATTAACATTCGCCTGATTATGATGGCAGCG
TTTACCAGTCTGAACCACGA

Product: DNA-binding transcriptional regulator GalS

Products: NA

Alternate protein names: Mgl repressor and galactose ultrainduction factor [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDVVMKAVTQLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDTVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCSALIVHSKALSDDELSDFMQHIPGMVLINRIVPGYAHRCVGLDN
VSGALMATRMLLNHGHQRIGYLSSNHGIEDDDMRREGWSKALQEQGIIAPDSWIGSGSPDMQGGEAAMVELLGRNLGLTA
VFAYNDSMAAGALTTLKDNGIVVPQHLSLIGFDDIPISRYTDPQLTTVRYPVMSMAKLATELALLGAAGKLDREATHCFM
PTLVRRHSVAQRQTVGPITN

Sequences:

>Translated_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDVVMKAVTQLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDTVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCSALIVHSKALSDDELSDFMQHIPGMVLINRIVPGYAHRCVGLDN
VSGALMATRMLLNHGHQRIGYLSSNHGIEDDDMRREGWSKALQEQGIIAPDSWIGSGSPDMQGGEAAMVELLGRNLGLTA
VFAYNDSMAAGALTTLKDNGIVVPQHLSLIGFDDIPISRYTDPQLTTVRYPVMSMAKLATELALLGAAGKLDREATHCFM
PTLVRRHSVAQRQTVGPITN
>Mature_340_residues
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDVVMKAVTQLGYRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKA
VDTVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCSALIVHSKALSDDELSDFMQHIPGMVLINRIVPGYAHRCVGLDN
VSGALMATRMLLNHGHQRIGYLSSNHGIEDDDMRREGWSKALQEQGIIAPDSWIGSGSPDMQGGEAAMVELLGRNLGLTA
VFAYNDSMAAGALTTLKDNGIVVPQHLSLIGFDDIPISRYTDPQLTTVRYPVMSMAKLATELALLGAAGKLDREATHCFM
PTLVRRHSVAQRQTVGPITN

Specific function: Repressor of the mgl operon. Binds galactose and D- fucose as inducers. GalS binds to an operator DNA sequence within its own coding sequence [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788474, Length=340, Percent_Identity=84.7058823529412, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1789202, Length=330, Percent_Identity=57.8787878787879, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1789068, Length=333, Percent_Identity=34.2342342342342, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1790369, Length=302, Percent_Identity=34.4370860927152, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=32.4324324324324, Blast_Score=168, Evalue=5e-43,
Organism=Escherichia coli, GI1790194, Length=304, Percent_Identity=29.6052631578947, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1787580, Length=318, Percent_Identity=28.3018867924528, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=302, Percent_Identity=25.8278145695364, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1786540, Length=298, Percent_Identity=30.2013422818792, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787906, Length=301, Percent_Identity=27.906976744186, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790715, Length=307, Percent_Identity=25.7328990228013, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1789456, Length=302, Percent_Identity=26.8211920529801, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1790689, Length=289, Percent_Identity=26.2975778546713, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36835; Mature: 36835

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDVVMKAVTQLGYRPNANAQALATQVSD
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDTVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCSALI
HHCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VHSKALSDDELSDFMQHIPGMVLINRIVPGYAHRCVGLDNVSGALMATRMLLNHGHQRIG
HHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHCC
YLSSNHGIEDDDMRREGWSKALQEQGIIAPDSWIGSGSPDMQGGEAAMVELLGRNLGLTA
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEE
VFAYNDSMAAGALTTLKDNGIVVPQHLSLIGFDDIPISRYTDPQLTTVRYPVMSMAKLAT
EEEECCCCHHCHHEEECCCCEECCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ELALLGAAGKLDREATHCFMPTLVRRHSVAQRQTVGPITN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITIRDVARQAGVSVATVSRVLNNSALVSPDTRDVVMKAVTQLGYRPNANAQALATQVSD
CCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
TIGVVVMDVSDAFFGALVKAVDTVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRCSALI
HHCEEEEECHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
VHSKALSDDELSDFMQHIPGMVLINRIVPGYAHRCVGLDNVSGALMATRMLLNHGHQRIG
HHHHCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHCC
YLSSNHGIEDDDMRREGWSKALQEQGIIAPDSWIGSGSPDMQGGEAAMVELLGRNLGLTA
EECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEE
VFAYNDSMAAGALTTLKDNGIVVPQHLSLIGFDDIPISRYTDPQLTTVRYPVMSMAKLAT
EEEECCCCHHCHHEEECCCCEECCCCCEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ELALLGAAGKLDREATHCFMPTLVRRHSVAQRQTVGPITN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 3146019 [H]