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The map label for this gene is lacA [C]

Identifier: 238895624

GI number: 238895624

Start: 3533042

End: 3533554

Strand: Reverse

Name: lacA [C]

Synonym: KP1_3712

Alternate gene names: 238895624

Gene position: 3533554-3533042 (Counterclockwise)

Preceding gene: 238895625

Following gene: 238895623

Centisome position: 67.32

GC content: 34.89

Gene sequence:

>513_bases
GTGTCACGCAAACAAGATAATCCCGATGTTCAAAGGAGTTTTTTAGTTTCCTTAATCAGATCCTTTATAAGAAATTGGAG
ATTTCAATTAATTAGATTTAAAAATAGAGGGAAAATTTCGCGGGGAAAAAATGCTTATGTTGGCCCAGGTGCAAATGTTT
ATATCCCTAATTTTGTAAAAATGGGTGATAACGTATCTATTGGTGCTGATTTTATTTCACAAGTGAATCTGACTATTGGA
AATGATAGTTTAATATCCTCACGTGTATCATTTATTGGTAACGACCATGATTTATTCAATGAGTCATCTTCAGCATATTT
CTCTGGAAGAAACAAGCCTGCCACTATAGTACTGGAAGGAGATAATTTTATCGGATTTGGTTCGGTTATTTTGGGAAATG
TCACGATAGGGAAAGGTGCTATTGTTGCAGCCTGTTCATTTGTTAATAAAGATGTCCCACCTAATTCTGTAGTAGGTGGG
GTACCTGCAAAAGTATTAAGAAATCGTTATTGA

Upstream 100 bases:

>100_bases
TTTCACGGGCGTCAATATAATATGTTTGACGGAGAAAAAATAGTTAATGATTGTAAACATAGGTATTCAGTTGAGACTAT
GGTTGATGGGGTAATTGAAA

Downstream 100 bases:

>100_bases
TTATAATTAATAGGAAACAAGAAATGACTAATGTAAAAAAAGCTCTGATTACCGGTATCACTGGGCAAGATGGTTCATAT
CTCGCAGAATTTCTGTTAGA

Product: galactoside O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MSRKQDNPDVQRSFLVSLIRSFIRNWRFQLIRFKNRGKISRGKNAYVGPGANVYIPNFVKMGDNVSIGADFISQVNLTIG
NDSLISSRVSFIGNDHDLFNESSSAYFSGRNKPATIVLEGDNFIGFGSVILGNVTIGKGAIVAACSFVNKDVPPNSVVGG
VPAKVLRNRY

Sequences:

>Translated_170_residues
MSRKQDNPDVQRSFLVSLIRSFIRNWRFQLIRFKNRGKISRGKNAYVGPGANVYIPNFVKMGDNVSIGADFISQVNLTIG
NDSLISSRVSFIGNDHDLFNESSSAYFSGRNKPATIVLEGDNFIGFGSVILGNVTIGKGAIVAACSFVNKDVPPNSVVGG
VPAKVLRNRY
>Mature_169_residues
SRKQDNPDVQRSFLVSLIRSFIRNWRFQLIRFKNRGKISRGKNAYVGPGANVYIPNFVKMGDNVSIGADFISQVNLTIGN
DSLISSRVSFIGNDHDLFNESSSAYFSGRNKPATIVLEGDNFIGFGSVILGNVTIGKGAIVAACSFVNKDVPPNSVVGGV
PAKVLRNRY

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=135, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1788345, Length=172, Percent_Identity=31.3953488372093, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 18560; Mature: 18429

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKQDNPDVQRSFLVSLIRSFIRNWRFQLIRFKNRGKISRGKNAYVGPGANVYIPNFVK
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEECCCCCEECCHHEE
MGDNVSIGADFISQVNLTIGNDSLISSRVSFIGNDHDLFNESSSAYFSGRNKPATIVLEG
CCCCCEECHHHHHEEEEEECCCHHHHHHHHHCCCCHHHCCCCCCEEECCCCCCEEEEEEC
DNFIGFGSVILGNVTIGKGAIVAACSFVNKDVPPNSVVGGVPAKVLRNRY
CCEEEECCEEEECEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SRKQDNPDVQRSFLVSLIRSFIRNWRFQLIRFKNRGKISRGKNAYVGPGANVYIPNFVK
CCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEECCCCCEECCHHEE
MGDNVSIGADFISQVNLTIGNDSLISSRVSFIGNDHDLFNESSSAYFSGRNKPATIVLEG
CCCCCEECHHHHHEEEEEECCCHHHHHHHHHCCCCHHHCCCCCCEEECCCCCCEEEEEEC
DNFIGFGSVILGNVTIGKGAIVAACSFVNKDVPPNSVVGGVPAKVLRNRY
CCEEEECCEEEECEEECCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]