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The map label for this gene is hisH [H]

Identifier: 238895600

GI number: 238895600

Start: 3504910

End: 3505500

Strand: Direct

Name: hisH [H]

Synonym: KP1_3684

Alternate gene names: 238895600

Gene position: 3504910-3505500 (Clockwise)

Preceding gene: 238895599

Following gene: 238895601

Centisome position: 66.78

GC content: 60.91

Gene sequence:

>591_bases
ATGAATGTGGTGATCCTTGATACCGGCTGCGCCAACCTCAATTCGGTGAAATCGGCGATTGGCCGCCACGGCTATGAGCC
GGTGGCCAGCCGCGATCCGGAAGTGGTATTGCGTGCCGATAAACTGTTTCTTCCCGGCGTCGGCACCGCCCAGGCGGCGA
TGGATCAGCTGCGCGACCGCGAGCTGATCGACCTGATCAAAGCCTGTACCCAGCCGGTGCTGGGGATTTGCTTGGGCATG
CAGCTGCTCGGCAAGCGCAGCGAGGAGAACAACGGCGTCGATCTGCTGGGCATTATCGAAGAAGAGGTGCCGAAGATGAC
CGACCACGGTCTGCCGCTGCCGCACATGGGCTGGAACCGGGTGTATGCCAAAGCTGGCGATCGGCTGTTCCGCGGCATTG
AAGAAGGCGCGTATTTCTACTTCGTGCACAGCTACGCCATGCCGGTCAACCCGTACACCATCGCCCAGTGCAATTACGGC
GAGGCGTTCACCGCCGCGGTGCAGAAAGATAACTTCTTTGGCGTGCAGTTTCACCCGGAGCGCTCCGGGAGCGCCGGGGC
GCAGCTGCTGAAAAACTTCCTGGAGATGTGA

Upstream 100 bases:

>100_bases
ACCACCGCGTGGAGAGCCTGTTCAAAGCCTTTGGCCGTACGCTGCGCCAGGCGATCCGCGTCCAGGGCGATGCCCTGCCG
TCTTCCAAAGGGGTGCTGTA

Downstream 100 bases:

>100_bases
TGATTATTCCTGCTTTAGATTTAATCGACGGCACCGTCGTTCGTCTCCATCAGGGCGATTACGGCCAGCAGCGCGACTAT
GGCAGCGATCCGCTGCCGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVASRDPEVVLRADKLFLPGVGTAQAAMDQLRDRELIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM

Sequences:

>Translated_196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVASRDPEVVLRADKLFLPGVGTAQAAMDQLRDRELIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM
>Mature_196_residues
MNVVILDTGCANLNSVKSAIGRHGYEPVASRDPEVVLRADKLFLPGVGTAQAAMDQLRDRELIDLIKACTQPVLGICLGM
QLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNRVYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYG
EAFTAAVQKDNFFGVQFHPERSGSAGAQLLKNFLEM

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=88.265306122449, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319725, Length=209, Percent_Identity=34.4497607655502, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.-

Molecular weight: Translated: 21590; Mature: 21590

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00442 GATASE_TYPE_I ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVILDTGCANLNSVKSAIGRHGYEPVASRDPEVVLRADKLFLPGVGTAQAAMDQLRDR
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHCCH
ELIDLIKACTQPVLGICLGMQLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHH
VYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYGEAFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCC
RSGSAGAQLLKNFLEM
CCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVVILDTGCANLNSVKSAIGRHGYEPVASRDPEVVLRADKLFLPGVGTAQAAMDQLRDR
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECEEECCCCCHHHHHHHHHCCH
ELIDLIKACTQPVLGICLGMQLLGKRSEENNGVDLLGIIEEEVPKMTDHGLPLPHMGWNR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCCCCCCHHH
VYAKAGDRLFRGIEEGAYFYFVHSYAMPVNPYTIAQCNYGEAFTAAVQKDNFFGVQFHPE
HHHHHHHHHHHHHHCCEEEEEEEEEECCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCC
RSGSAGAQLLKNFLEM
CCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]