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The map label for this gene is amyA [H]

Identifier: 238895479

GI number: 238895479

Start: 3365293

End: 3366780

Strand: Direct

Name: amyA [H]

Synonym: KP1_3550

Alternate gene names: 238895479

Gene position: 3365293-3366780 (Clockwise)

Preceding gene: 238895473

Following gene: 238895481

Centisome position: 64.12

GC content: 55.44

Gene sequence:

>1488_bases
ATGAAAAACCCCACCTTATTGCAATGTTTTCACTGGTACTACCCCACCGGCGGCGAGCTGTGGCCTGAGGTCGAAGCGCT
GGCGCCCAGCCTGAATGAAATCGGCATCAATATGGTCTGGTTGCCACCCGCCTACAAAGGCGCCTCGGGCGGTTACTCCG
TCGGCTATGATACCTACGATCTCTTTGACCTCGGCGAGTTTGATCAAAAAGGGTCTGTCGCCACCAAATATGGCGACAAA
GCGCAGTTGCTGGCGGCGATCAACGCCCTGAAGGAGCACAATATCGCCGTACTGCTGGATGTGGTGGTAAACCATAAAAT
GGGCGCTGATGAAAAAGAGGCTCTGCGCGTTCAGCGCGTCGATGAGCAGGATCGTACGCAAATTGACGAGGAGATCATTG
AGTGCGAGGCATGGACGCGCTACACCTTCCCGGTGCGCGCCGGACAGTATTCGCAGTTTGTCTGGGACTACAAATGTTTT
AGCGGCATTGACCACATCGAGAACCCGACGGAAGACGGTGTGTTTAAAATCGTCAACGATTACACCGGCGAAGGCTGGAA
TGAACAGGTCGATGATGAGCTCGGCAACTTTGACTATCTGATGGGCGCCAATATCGATTTCCGCAATCATGCAGTGACCG
AGGAGATCAAATACTGGGCGCGCTGGGTAATGGAGCAGACCGGCTGCGACGGTTTTCGTCTTGACGCGGTCAAGCATATC
CCCGCCTGGTTTTATAAAGCGTGGATCGAGCATGTTCAGGAGGTGGCCCCGCAGCCGCTGTTTATTGTAGCGGAATACTG
GTCCCATGAAGTGGAGAAGCTCCAGCAGTATATCGACCTGGTCGAGGGCAAAACGATGCTGTTTGATGCCCCACTGCAGA
TGAAATTTCATGAAGCGTCGCGCCAGGGCCGCGATTATGACATGAGCCAGATTTTCAGCGGGACGCTGGTCGAAGCCGAC
CCTTTCCATGCCGTCACTCTGGTCACCAATCATGATACGCAGCCGCTGCAGGCCCTCGAGGCGCCGGTTGAACCCTGGTT
CAAGCCCCTCGCCTATGCGCTGATCCTGCTGCGGGAGAATGGCGTGCCCTCGGTCTTTTATGCCGATCTCTTCGGCGCTT
CCTATGAGGATACCGGCGGCGACGGAGAGACCTATGCCATTGAGATGCCCGTTATCGAACAACTCCACGAGCTGATCGAC
GCCCGTCAGCGTTTTGCCCATGGCGTGCAAACCCTTTGGTTCGATCATCCTAACTGCATCGCCTTTAGTCGTAGCGGTAC
TGACGACGATCCAGGCTGCGTGGTGATCATGTCCAATGGCGATGAGGGCGAAAAGACGCTGACGCTGGGCGAAAACTATG
GCAACAAACGCTGGCGGGATTTTCTCGGTAACCGGGAAGAGATTGTCGAAACCGATGGCGAAGGCTGCGCGACCTTTACA
TGTAACGGGGGGAGCGTGAGCGTGTGGGTGTTAGAGGAGGTATTGTAG

Upstream 100 bases:

>100_bases
CCGCTTTAGGGTATAGCGAAAATCAGGGCTTTCCTCACCCTTTTTGCTGGATATCTCCCATACTGCAAAGGTCAGCCTGT
TGACGTCTGGAGCATGGAAG

Downstream 100 bases:

>100_bases
TCCTCTGACGCCGGGCGGGCCAGCTATGGCCCGCCAAAATCTCAGCGCGTGGAGGCCGGCGCGCTGACGGGCCCCGTCGC
TCGCGCTTTGGCCCACGCCT

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKNPTLLQCFHWYYPTGGELWPEVEALAPSLNEIGINMVWLPPAYKGASGGYSVGYDTYDLFDLGEFDQKGSVATKYGDK
AQLLAAINALKEHNIAVLLDVVVNHKMGADEKEALRVQRVDEQDRTQIDEEIIECEAWTRYTFPVRAGQYSQFVWDYKCF
SGIDHIENPTEDGVFKIVNDYTGEGWNEQVDDELGNFDYLMGANIDFRNHAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
PAWFYKAWIEHVQEVAPQPLFIVAEYWSHEVEKLQQYIDLVEGKTMLFDAPLQMKFHEASRQGRDYDMSQIFSGTLVEAD
PFHAVTLVTNHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELID
ARQRFAHGVQTLWFDHPNCIAFSRSGTDDDPGCVVIMSNGDEGEKTLTLGENYGNKRWRDFLGNREEIVETDGEGCATFT
CNGGSVSVWVLEEVL

Sequences:

>Translated_495_residues
MKNPTLLQCFHWYYPTGGELWPEVEALAPSLNEIGINMVWLPPAYKGASGGYSVGYDTYDLFDLGEFDQKGSVATKYGDK
AQLLAAINALKEHNIAVLLDVVVNHKMGADEKEALRVQRVDEQDRTQIDEEIIECEAWTRYTFPVRAGQYSQFVWDYKCF
SGIDHIENPTEDGVFKIVNDYTGEGWNEQVDDELGNFDYLMGANIDFRNHAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
PAWFYKAWIEHVQEVAPQPLFIVAEYWSHEVEKLQQYIDLVEGKTMLFDAPLQMKFHEASRQGRDYDMSQIFSGTLVEAD
PFHAVTLVTNHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELID
ARQRFAHGVQTLWFDHPNCIAFSRSGTDDDPGCVVIMSNGDEGEKTLTLGENYGNKRWRDFLGNREEIVETDGEGCATFT
CNGGSVSVWVLEEVL
>Mature_495_residues
MKNPTLLQCFHWYYPTGGELWPEVEALAPSLNEIGINMVWLPPAYKGASGGYSVGYDTYDLFDLGEFDQKGSVATKYGDK
AQLLAAINALKEHNIAVLLDVVVNHKMGADEKEALRVQRVDEQDRTQIDEEIIECEAWTRYTFPVRAGQYSQFVWDYKCF
SGIDHIENPTEDGVFKIVNDYTGEGWNEQVDDELGNFDYLMGANIDFRNHAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
PAWFYKAWIEHVQEVAPQPLFIVAEYWSHEVEKLQQYIDLVEGKTMLFDAPLQMKFHEASRQGRDYDMSQIFSGTLVEAD
PFHAVTLVTNHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELID
ARQRFAHGVQTLWFDHPNCIAFSRSGTDDDPGCVVIMSNGDEGEKTLTLGENYGNKRWRDFLGNREEIVETDGEGCATFT
CNGGSVSVWVLEEVL

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=495, Percent_Identity=86.4646464646465, Blast_Score=904, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56235; Mature: 56235

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPTLLQCFHWYYPTGGELWPEVEALAPSLNEIGINMVWLPPAYKGASGGYSVGYDTYD
CCCCCEEEEEEEECCCCCCCCCCHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCCCEE
LFDLGEFDQKGSVATKYGDKAQLLAAINALKEHNIAVLLDVVVNHKMGADEKEALRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCHHHHHHHHHC
DEQDRTQIDEEIIECEAWTRYTFPVRAGQYSQFVWDYKCFSGIDHIENPTEDGVFKIVND
CHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEEHHHHHCCCHHCCCCCCCCCEEEECC
YTGEGWNEQVDDELGNFDYLMGANIDFRNHAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
CCCCCCCCHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
PAWFYKAWIEHVQEVAPQPLFIVAEYWSHEVEKLQQYIDLVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
RQGRDYDMSQIFSGTLVEADPFHAVTLVTNHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHHCCCEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEECC
GVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELIDARQRFAHGVQTLWFDHPNCI
CCCCEEEHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
AFSRSGTDDDPGCVVIMSNGDEGEKTLTLGENYGNKRWRDFLGNREEIVETDGEGCATFT
EEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCCEEEEE
CNGGSVSVWVLEEVL
ECCCCEEEEEEECCC
>Mature Secondary Structure
MKNPTLLQCFHWYYPTGGELWPEVEALAPSLNEIGINMVWLPPAYKGASGGYSVGYDTYD
CCCCCEEEEEEEECCCCCCCCCCHHHHCCCHHHCCEEEEECCCCCCCCCCCCCCCCCCEE
LFDLGEFDQKGSVATKYGDKAQLLAAINALKEHNIAVLLDVVVNHKMGADEKEALRVQRV
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCEEEEEEHHCCCCCCCHHHHHHHHHC
DEQDRTQIDEEIIECEAWTRYTFPVRAGQYSQFVWDYKCFSGIDHIENPTEDGVFKIVND
CHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHEEHHHHHCCCHHCCCCCCCCCEEEECC
YTGEGWNEQVDDELGNFDYLMGANIDFRNHAVTEEIKYWARWVMEQTGCDGFRLDAVKHI
CCCCCCCCHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHH
PAWFYKAWIEHVQEVAPQPLFIVAEYWSHEVEKLQQYIDLVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
RQGRDYDMSQIFSGTLVEADPFHAVTLVTNHDTQPLQALEAPVEPWFKPLAYALILLREN
CCCCCCCHHHHHCCCEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEECC
GVPSVFYADLFGASYEDTGGDGETYAIEMPVIEQLHELIDARQRFAHGVQTLWFDHPNCI
CCCCEEEHHHHCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHEEEECCCCEE
AFSRSGTDDDPGCVVIMSNGDEGEKTLTLGENYGNKRWRDFLGNREEIVETDGEGCATFT
EEECCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHCCCHHHHHHCCCCCEEEEE
CNGGSVSVWVLEEVL
ECCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 9097040; 9278503; 1527488; 8371104 [H]