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The map label for this gene is hsrA [H]

Identifier: 238895462

GI number: 238895462

Start: 3349858

End: 3351279

Strand: Direct

Name: hsrA [H]

Synonym: KP1_3532

Alternate gene names: 238895462

Gene position: 3349858-3351279 (Clockwise)

Preceding gene: 238895460

Following gene: 238895464

Centisome position: 63.82

GC content: 60.48

Gene sequence:

>1422_bases
ATGAATACCGCGATCTCTTCCCGCGATGAACGTGCTTTTTCAGCGCCGGCGCTGCTGGTGGCCGGTGCCTTTTTTATGGA
GTTTCTCGACGGGACGGTGATCGCCACCGCCCTGCCCGATATGGCAAGGGATTTTGGCGTCACCGCGGTCGAACTGAATA
TCGGCATCAGCGCCTATCTGATTACCCTTGCGGTCCTGATCCCGGCCAGCGGCTGGATAGCCGATCGCTTCGGCGCCCGG
GCTATTTTTACTCTCGCCCTGGCGATCTTTACCCTTGCCTCGGTCTTCTGCGGCCTCTCCACCGAGGTGCATATCTTTGT
CGCCATGCGTATCCTTCAGGGAGTCGGCGGCGCGCTGATGGTCCCCGTTGGTCGCCTTGCGGTGCTACGCACCACGCCGA
AACACCAGCTGATCAAGGCCATCGCCACCCTGACCTGGCCGGCGCTGGTGGCCCCCATTATCGGCCCGCCTTTGGGCGGC
TTTATAACCCGCTACGCCAGCTGGCACTGGATCTTCTTTATCAATGTCCCGCTGGGCCTTGCCGCCATCATCCTTTCGCT
GCGCATTATCCCTGATATTCGCGAAACGGAACGGCGCTCCTTCGATCTCAGCGGATTTATCACTACCTCGGTCGCGATGG
TCAGTCTGGTGACCGCGATGGAGCGGCTCGGCGATCGCCAGCCGCAGATCTGGCCGACGCTGGCTCTCGCCGCGCTTGGC
TTCGGCTGTCTGTTATATTCCATTCGTCATTTCCGCCGGGCGGCGGCGCCTATGGTTCGTCTCGACGCCCTGCAGGTGCC
GACGTTCCGGGTGACAATGTACGGCGGCTCGTTGTTCCGGGCCTCCATCAGCGCGGTGCCTTTTCTGCTCCCCCTGCTGT
TCCAGGTGGGATTCGGTATGGATCCTTTTCATTCCGGCCTGCTGGTGCTGGCGGTGTTTGTCGGTAATTTAACCATTAAA
CCCGCCACCACACCGCTCATTCGCTGGCTGGGCTTTCGTCGGCTATTGCTGATCAATGGCGCGTTGAACGTCTGTTCGCT
GCTGGCTTGCGCGCTGCTTACGCCGCAGACGCCGGTCTGGGCCATCATGCTGATCCTTTATCTCGGCGGGGTGTTTCGCT
CAATCCAGTTTACCGGTGTAAGCACGCTGGCCTTCGCCGATGTCCCCGCCGCGCAGATGAGTGATGCTAATACGCTGTTC
AGTACCGCGTCGCAGCTGGCGGTAGGGCTGGGGATCACGCTCGGGGCGATTGGCATTCGCCTGGGCGAGCAGGTCGGCGA
CTGGCTGCATCTTACTGAACTACCGGGGATCAGCTTCCGCCTGTCGTTTGTGTTTATCGCCTTGATCTGTCTGGTAGGGA
TGATCGACAGCCTGCATCTGGCCAAAACGGCCGGCAGCAGCGTGTCGGAGAAGAAGAAATAG

Upstream 100 bases:

>100_bases
AATAAAACCTGCCGTCGCAGGTTTTTTTATCTACCCTACTGGCTGGCGGGTCGTCTCCTGCCGCTTTGCACAGGATCACA
CTCTTGATGGCAGTGACAAC

Downstream 100 bases:

>100_bases
AAGAAAAAATAAACGACGGAAGGGCCAGCGGCGCTGGCCCTGATGACGGTTAAGCGACGATCTGGCGAACAAAGTCTTCG
ATTGCTTTATCCTGGCAGTT

Product: putative MFS-family transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MNTAISSRDERAFSAPALLVAGAFFMEFLDGTVIATALPDMARDFGVTAVELNIGISAYLITLAVLIPASGWIADRFGAR
AIFTLALAIFTLASVFCGLSTEVHIFVAMRILQGVGGALMVPVGRLAVLRTTPKHQLIKAIATLTWPALVAPIIGPPLGG
FITRYASWHWIFFINVPLGLAAIILSLRIIPDIRETERRSFDLSGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALG
FGCLLYSIRHFRRAAAPMVRLDALQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGMDPFHSGLLVLAVFVGNLTIK
PATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVWAIMLILYLGGVFRSIQFTGVSTLAFADVPAAQMSDANTLF
STASQLAVGLGITLGAIGIRLGEQVGDWLHLTELPGISFRLSFVFIALICLVGMIDSLHLAKTAGSSVSEKKK

Sequences:

>Translated_473_residues
MNTAISSRDERAFSAPALLVAGAFFMEFLDGTVIATALPDMARDFGVTAVELNIGISAYLITLAVLIPASGWIADRFGAR
AIFTLALAIFTLASVFCGLSTEVHIFVAMRILQGVGGALMVPVGRLAVLRTTPKHQLIKAIATLTWPALVAPIIGPPLGG
FITRYASWHWIFFINVPLGLAAIILSLRIIPDIRETERRSFDLSGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALG
FGCLLYSIRHFRRAAAPMVRLDALQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGMDPFHSGLLVLAVFVGNLTIK
PATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVWAIMLILYLGGVFRSIQFTGVSTLAFADVPAAQMSDANTLF
STASQLAVGLGITLGAIGIRLGEQVGDWLHLTELPGISFRLSFVFIALICLVGMIDSLHLAKTAGSSVSEKKK
>Mature_473_residues
MNTAISSRDERAFSAPALLVAGAFFMEFLDGTVIATALPDMARDFGVTAVELNIGISAYLITLAVLIPASGWIADRFGAR
AIFTLALAIFTLASVFCGLSTEVHIFVAMRILQGVGGALMVPVGRLAVLRTTPKHQLIKAIATLTWPALVAPIIGPPLGG
FITRYASWHWIFFINVPLGLAAIILSLRIIPDIRETERRSFDLSGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALG
FGCLLYSIRHFRRAAAPMVRLDALQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGMDPFHSGLLVLAVFVGNLTIK
PATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVWAIMLILYLGGVFRSIQFTGVSTLAFADVPAAQMSDANTLF
STASQLAVGLGITLGAIGIRLGEQVGDWLHLTELPGISFRLSFVFIALICLVGMIDSLHLAKTAGSSVSEKKK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=471, Percent_Identity=33.7579617834395, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1788392, Length=404, Percent_Identity=36.6336633663366, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI1789042, Length=166, Percent_Identity=33.7349397590361, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87081983, Length=290, Percent_Identity=27.9310344827586, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=26.2135922330097, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790146, Length=166, Percent_Identity=31.3253012048193, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082320, Length=359, Percent_Identity=22.5626740947075, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=26.6666666666667, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50812; Mature: 50812

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTAISSRDERAFSAPALLVAGAFFMEFLDGTVIATALPDMARDFGVTAVELNIGISAYL
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCHHHHH
ITLAVLIPASGWIADRFGARAIFTLALAIFTLASVFCGLSTEVHIFVAMRILQGVGGALM
HHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
VPVGRLAVLRTTPKHQLIKAIATLTWPALVAPIIGPPLGGFITRYASWHWIFFINVPLGL
CCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEEECHHHH
AAIILSLRIIPDIRETERRSFDLSGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALG
HHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FGCLLYSIRHFRRAAAPMVRLDALQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGM
HHHHHHHHHHHHHHHCCHHHHHHEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
DPFHSGLLVLAVFVGNLTIKPATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVW
CHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AIMLILYLGGVFRSIQFTGVSTLAFADVPAAQMSDANTLFSTASQLAVGLGITLGAIGIR
HHHHHHHHHHHHHHHEECCCHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGEQVGDWLHLTELPGISFRLSFVFIALICLVGMIDSLHLAKTAGSSVSEKKK
HHHHHCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTAISSRDERAFSAPALLVAGAFFMEFLDGTVIATALPDMARDFGVTAVELNIGISAYL
CCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEECCHHHHH
ITLAVLIPASGWIADRFGARAIFTLALAIFTLASVFCGLSTEVHIFVAMRILQGVGGALM
HHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEE
VPVGRLAVLRTTPKHQLIKAIATLTWPALVAPIIGPPLGGFITRYASWHWIFFINVPLGL
CCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEEECHHHH
AAIILSLRIIPDIRETERRSFDLSGFITTSVAMVSLVTAMERLGDRQPQIWPTLALAALG
HHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
FGCLLYSIRHFRRAAAPMVRLDALQVPTFRVTMYGGSLFRASISAVPFLLPLLFQVGFGM
HHHHHHHHHHHHHHHCCHHHHHHEECCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCC
DPFHSGLLVLAVFVGNLTIKPATTPLIRWLGFRRLLLINGALNVCSLLACALLTPQTPVW
CHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
AIMLILYLGGVFRSIQFTGVSTLAFADVPAAQMSDANTLFSTASQLAVGLGITLGAIGIR
HHHHHHHHHHHHHHHEECCCHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGEQVGDWLHLTELPGISFRLSFVFIALICLVGMIDSLHLAKTAGSSVSEKKK
HHHHHCCEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]