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The map label for this gene is msbB [H]

Identifier: 238895432

GI number: 238895432

Start: 3319078

End: 3320052

Strand: Reverse

Name: msbB [H]

Synonym: KP1_3500

Alternate gene names: 238895432

Gene position: 3320052-3319078 (Counterclockwise)

Preceding gene: 238895433

Following gene: 238895429

Centisome position: 63.26

GC content: 56.82

Gene sequence:

>975_bases
ATGGAAACGAAAAAAAATAATATTGAGTTTATCCCTAAGTTTGAAAAATCCTTTTTACTGCCGCGCTACTGGGGCGCCTG
GCTGGGGGTCTTTGCCTTCGCCGGTATTGCGCTGACGCCGCCATCCTTTCGCGATCCGCTCCTCGGCAAACTCGGTCGTC
TGGTAGGGCGTCTGGCGAAAAGCTCGCGCCGCCGGGCGCAGATTAATTTGCTGTACTGCTTCCCGGAAAAAAGCGAGTAC
GAGCGGGAAGCGATTATCGACGCCATGTATGCCTCGGCGCCGCAGGCGATGGTGATGATGGCCGAGCTGGGTCTGCGCGA
TCCGCAGAAAATCCTTGCCCGCGTCGACTGGCAGGGGAAAGCGATCATCGACGAGATGCAGCGCAATAATGAGAAGGTTA
TTTTTCTCGTTCCGCATGCATGGGGTGTGGATATCCCGGCGATGCTAATGGCCTCCGGCGGGCAGAAGATGGCGGCGATG
TTCCATAACCAGGGTAACCCGGTGTTCGATTACGTGTGGAATACCGTGCGTCGTCGTTTTGGCGGGCGTATGCACGCGCG
CAACGATGGGATCAAACCGTTTATTCAGTCGGTGCGCCAGGGCTACTGGGGCTACTATCTGCCCGATCAGGATCACGGCG
CCGAACACAGCGAGTTTGTGGATTTCTTTGCCACCTACAAGGCCACGCTGCCGGCGATTGGCCGCTTAATGAAAGTATGC
CGCGCCCGCGTCGTACCGCTGTTCCCGGTCTACGATGGCAAGACGCACCGCCTGACGGTGCTGGTGCGCCCGCCGATGGA
CGATCTGCTGGACGCTGACGATACGACCATCGCCCGGCGGATGAACGAAGAAGTGGAAGTGTTTGTTAAGCCGCACACCG
AGCAGTATACCTGGATCCTGAAGCTGCTGAAAACGCGCAAGCCGGGTGAAATCGAGCCCTATAAACGCAAAGAGCTGTTC
ACGAAAAAGAAATAA

Upstream 100 bases:

>100_bases
GCCGGTGCCCCAGGGCGCCGGCTTTTTCTTTTGTAGGATACCCGACGTCTCGCTACACTATCCCATTATCTTGATTAAGC
AGTCGATCTGCGGATTGGGC

Downstream 100 bases:

>100_bases
AAAAAGCCTCCGTCAGGAGGCTTTTTTATCTCTACCGTGCTTACTCGACGGTGAGGATACGGGTGGTGTTGGTGCTGCCG
ATGGTGCTCATCACGTCACC

Product: lipid A biosynthesis (KDO)2-(lauroyl)-lipid IVA acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
METKKNNIEFIPKFEKSFLLPRYWGAWLGVFAFAGIALTPPSFRDPLLGKLGRLVGRLAKSSRRRAQINLLYCFPEKSEY
EREAIIDAMYASAPQAMVMMAELGLRDPQKILARVDWQGKAIIDEMQRNNEKVIFLVPHAWGVDIPAMLMASGGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRMHARNDGIKPFIQSVRQGYWGYYLPDQDHGAEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPVYDGKTHRLTVLVRPPMDDLLDADDTTIARRMNEEVEVFVKPHTEQYTWILKLLKTRKPGEIEPYKRKELF
TKKK

Sequences:

>Translated_324_residues
METKKNNIEFIPKFEKSFLLPRYWGAWLGVFAFAGIALTPPSFRDPLLGKLGRLVGRLAKSSRRRAQINLLYCFPEKSEY
EREAIIDAMYASAPQAMVMMAELGLRDPQKILARVDWQGKAIIDEMQRNNEKVIFLVPHAWGVDIPAMLMASGGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRMHARNDGIKPFIQSVRQGYWGYYLPDQDHGAEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPVYDGKTHRLTVLVRPPMDDLLDADDTTIARRMNEEVEVFVKPHTEQYTWILKLLKTRKPGEIEPYKRKELF
TKKK
>Mature_324_residues
METKKNNIEFIPKFEKSFLLPRYWGAWLGVFAFAGIALTPPSFRDPLLGKLGRLVGRLAKSSRRRAQINLLYCFPEKSEY
EREAIIDAMYASAPQAMVMMAELGLRDPQKILARVDWQGKAIIDEMQRNNEKVIFLVPHAWGVDIPAMLMASGGQKMAAM
FHNQGNPVFDYVWNTVRRRFGGRMHARNDGIKPFIQSVRQGYWGYYLPDQDHGAEHSEFVDFFATYKATLPAIGRLMKVC
RARVVPLFPVYDGKTHRLTVLVRPPMDDLLDADDTTIARRMNEEVEVFVKPHTEQYTWILKLLKTRKPGEIEPYKRKELF
TKKK

Specific function: Transfers myristate or laurate, activated on ACP, to (KDO)2-(lauroyl)-lipid IVA [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1788161, Length=323, Percent_Identity=83.28173374613, Blast_Score=570, Evalue=1e-164,
Organism=Escherichia coli, GI1787292, Length=309, Percent_Identity=29.126213592233, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI87082094, Length=307, Percent_Identity=27.0358306188925, Blast_Score=101, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011921 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 37458; Mature: 37458

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKKNNIEFIPKFEKSFLLPRYWGAWLGVFAFAGIALTPPSFRDPLLGKLGRLVGRLAK
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHH
SSRRRAQINLLYCFPEKSEYEREAIIDAMYASAPQAMVMMAELGLRDPQKILARVDWQGK
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCH
AIIDEMQRNNEKVIFLVPHAWGVDIPAMLMASGGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
GGRMHARNDGIKPFIQSVRQGYWGYYLPDQDHGAEHSEFVDFFATYKATLPAIGRLMKVC
CCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPVYDGKTHRLTVLVRPPMDDLLDADDTTIARRMNEEVEVFVKPHTEQYTWIL
HHHCCEEEEECCCCCEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCHHHHHHH
KLLKTRKPGEIEPYKRKELFTKKK
HHHHCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
METKKNNIEFIPKFEKSFLLPRYWGAWLGVFAFAGIALTPPSFRDPLLGKLGRLVGRLAK
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHH
SSRRRAQINLLYCFPEKSEYEREAIIDAMYASAPQAMVMMAELGLRDPQKILARVDWQGK
HCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHCCCCCH
AIIDEMQRNNEKVIFLVPHAWGVDIPAMLMASGGQKMAAMFHNQGNPVFDYVWNTVRRRF
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
GGRMHARNDGIKPFIQSVRQGYWGYYLPDQDHGAEHSEFVDFFATYKATLPAIGRLMKVC
CCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
RARVVPLFPVYDGKTHRLTVLVRPPMDDLLDADDTTIARRMNEEVEVFVKPHTEQYTWIL
HHHCCEEEEECCCCCEEEEEEECCCHHHHHCCCCHHHHHHCCCCEEEEECCCCHHHHHHH
KLLKTRKPGEIEPYKRKELFTKKK
HHHHCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]