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The map label for this gene is yjiO [H]

Identifier: 238895142

GI number: 238895142

Start: 3023406

End: 3024638

Strand: Direct

Name: yjiO [H]

Synonym: KP1_3182

Alternate gene names: 238895142

Gene position: 3023406-3024638 (Clockwise)

Preceding gene: 238895141

Following gene: 238895143

Centisome position: 57.6

GC content: 54.01

Gene sequence:

>1233_bases
ATGAAATTAGCGTTGGGACCGTACATCCGTTTTAGCAGAATCTTGTTATTTCCGCTGGCGATGGTTATCTATGATTTTTC
TGCTTATCTGACTACCGATCTCATTCAGCCGGGTATTATCCACATCATCCGGGAATTTGAGGCTGATGTTGCGTTAGCCC
CGGCCTCTGTCAGCCTCTATTTAGCCGGGGGGCTGGCATTACAGTGGCTGCTGGGGCCGCTCTCCGACCGCATTGGTCGC
CGCCCCGTACTACTGACCGGAGCCATCATCTTTTCTTTGGCCTGCTTCAGCATGATCTTTGTCACGTCGATTGATCAATA
TCTTATCGCCCGTTTTATCCAGGGAACCAGTATCTGCTTCATTTCGACAGTCGGTTATGTCTCCATCCAGGAGGCTTTTG
ATGAAAAAGAGTCTATCCGCATTATGGCGGCGCTGACATCCATCGTTTTGCTGGCGCCGGTAATCGGGCCCCTGGCGGGC
GCTGGCCTGATGAATTTCCTGCACTGGAAGTTATTGTTTGCGATCATCGGCGCCATGAGCTTGCTGGCCTGGGCGTTACT
GATTTTTAACATGCCAGAAACGGTCACCTCTCAGGGCCGGGGGTTTCGTCCGGGAGAGGTATTTTCCGAGTTTGTCCGCG
CCTTTAAACAGCCGGTGGTGCTCACGGGCGCGCTGGCGTTGTCATTCAGTAACCTGCCGATAATCACCTGGGTAGCACTC
TCTCCGGTGATCCTGATCGATGACGGCGGAATGTCGCGCGGCGCCTATGCCTGGACCCAGGTGCCTGTCTTTGGCGGCGT
TATTATTGCCAGCATCATCGTGGCCCGTTTTATCAAGGAACCGACCTCTCCCCGCTTTATCTGGCGCACTATCCCGATAC
AGCTGACCGGGTTGTTGGTATTACTGGCGGGAAATATTGCATGGCCCCATACCTGGTGGTGGTCCGTTTCAGGAACAAGT
CTCTATGCCCTCGGAACAGGCCTGTTGTTCCCCGTATTATTCCGTTTTGCGCTTTTTTCCCACTCCCTGCCGAAAGGGAC
GGTTTCAGCCACCATCAACATTGTGGCGCTCAGTTTTATGGCGGCCTCGGTGGAAGTAGCGCGCTGGGTTTACTTCCAGG
CTGGCGGCAGAATAGCCTTTCACTGCCTGGCGCTGATAGCGGGAATCGTCGTCATCATGCTGGTCTCGCGCTTGTTAAAA
CTCCGTCAACAACATCTCCTTCAGCCAGCCTGA

Upstream 100 bases:

>100_bases
TTGTCATTCCGCGGCCAGCCTGTGACGACAGCCGCAAGGGATCCACATCAACCAGGCCCCGATACCAGGGAAACCGTCAC
CGACCAGGGAGTCTTCGCGT

Downstream 100 bases:

>100_bases
AGGTTTAGGCCTTTTTGCCCGCAACAGGAGAACACTATGTTTTCATACATTATGCTGGGAACGAACGATCTGCCACGAGC
CATCAAGTTCTATGATCCGT

Product: putative sugar transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKLALGPYIRFSRILLFPLAMVIYDFSAYLTTDLIQPGIIHIIREFEADVALAPASVSLYLAGGLALQWLLGPLSDRIGR
RPVLLTGAIIFSLACFSMIFVTSIDQYLIARFIQGTSICFISTVGYVSIQEAFDEKESIRIMAALTSIVLLAPVIGPLAG
AGLMNFLHWKLLFAIIGAMSLLAWALLIFNMPETVTSQGRGFRPGEVFSEFVRAFKQPVVLTGALALSFSNLPIITWVAL
SPVILIDDGGMSRGAYAWTQVPVFGGVIIASIIVARFIKEPTSPRFIWRTIPIQLTGLLVLLAGNIAWPHTWWWSVSGTS
LYALGTGLLFPVLFRFALFSHSLPKGTVSATINIVALSFMAASVEVARWVYFQAGGRIAFHCLALIAGIVVIMLVSRLLK
LRQQHLLQPA

Sequences:

>Translated_410_residues
MKLALGPYIRFSRILLFPLAMVIYDFSAYLTTDLIQPGIIHIIREFEADVALAPASVSLYLAGGLALQWLLGPLSDRIGR
RPVLLTGAIIFSLACFSMIFVTSIDQYLIARFIQGTSICFISTVGYVSIQEAFDEKESIRIMAALTSIVLLAPVIGPLAG
AGLMNFLHWKLLFAIIGAMSLLAWALLIFNMPETVTSQGRGFRPGEVFSEFVRAFKQPVVLTGALALSFSNLPIITWVAL
SPVILIDDGGMSRGAYAWTQVPVFGGVIIASIIVARFIKEPTSPRFIWRTIPIQLTGLLVLLAGNIAWPHTWWWSVSGTS
LYALGTGLLFPVLFRFALFSHSLPKGTVSATINIVALSFMAASVEVARWVYFQAGGRIAFHCLALIAGIVVIMLVSRLLK
LRQQHLLQPA
>Mature_410_residues
MKLALGPYIRFSRILLFPLAMVIYDFSAYLTTDLIQPGIIHIIREFEADVALAPASVSLYLAGGLALQWLLGPLSDRIGR
RPVLLTGAIIFSLACFSMIFVTSIDQYLIARFIQGTSICFISTVGYVSIQEAFDEKESIRIMAALTSIVLLAPVIGPLAG
AGLMNFLHWKLLFAIIGAMSLLAWALLIFNMPETVTSQGRGFRPGEVFSEFVRAFKQPVVLTGALALSFSNLPIITWVAL
SPVILIDDGGMSRGAYAWTQVPVFGGVIIASIIVARFIKEPTSPRFIWRTIPIQLTGLLVLLAGNIAWPHTWWWSVSGTS
LYALGTGLLFPVLFRFALFSHSLPKGTVSATINIVALSFMAASVEVARWVYFQAGGRIAFHCLALIAGIVVIMLVSRLLK
LRQQHLLQPA

Specific function: Confers resistance to acriflavine, chloramphenicol, norfloxacin, ethidium bromide and TPP [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1790794, Length=398, Percent_Identity=59.2964824120603, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI1787065, Length=407, Percent_Identity=38.3292383292383, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI48994889, Length=223, Percent_Identity=31.390134529148, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790146, Length=368, Percent_Identity=25.8152173913043, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788509, Length=252, Percent_Identity=23.8095238095238, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44842; Mature: 44842

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLALGPYIRFSRILLFPLAMVIYDFSAYLTTDLIQPGIIHIIREFEADVALAPASVSLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCEEEE
LAGGLALQWLLGPLSDRIGRRPVLLTGAIIFSLACFSMIFVTSIDQYLIARFIQGTSICF
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISTVGYVSIQEAFDEKESIRIMAALTSIVLLAPVIGPLAGAGLMNFLHWKLLFAIIGAMS
EECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAWALLIFNMPETVTSQGRGFRPGEVFSEFVRAFKQPVVLTGALALSFSNLPIITWVAL
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHEECCCCEEEEHHH
SPVILIDDGGMSRGAYAWTQVPVFGGVIIASIIVARFIKEPTSPRFIWRTIPIQLTGLLV
CCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
LLAGNIAWPHTWWWSVSGTSLYALGTGLLFPVLFRFALFSHSLPKGTVSATINIVALSFM
HHHCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AASVEVARWVYFQAGGRIAFHCLALIAGIVVIMLVSRLLKLRQQHLLQPA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLALGPYIRFSRILLFPLAMVIYDFSAYLTTDLIQPGIIHIIREFEADVALAPASVSLY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEECCCCEEEE
LAGGLALQWLLGPLSDRIGRRPVLLTGAIIFSLACFSMIFVTSIDQYLIARFIQGTSICF
EHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ISTVGYVSIQEAFDEKESIRIMAALTSIVLLAPVIGPLAGAGLMNFLHWKLLFAIIGAMS
EECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLAWALLIFNMPETVTSQGRGFRPGEVFSEFVRAFKQPVVLTGALALSFSNLPIITWVAL
HHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEHHHHHEECCCCEEEEHHH
SPVILIDDGGMSRGAYAWTQVPVFGGVIIASIIVARFIKEPTSPRFIWRTIPIQLTGLLV
CCEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
LLAGNIAWPHTWWWSVSGTSLYALGTGLLFPVLFRFALFSHSLPKGTVSATINIVALSFM
HHHCCCCCCCCEEEEECCCEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AASVEVARWVYFQAGGRIAFHCLALIAGIVVIMLVSRLLKLRQQHLLQPA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]