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The map label for this gene is rnt [H]

Identifier: 238895034

GI number: 238895034

Start: 2918791

End: 2919384

Strand: Direct

Name: rnt [H]

Synonym: KP1_3064

Alternate gene names: 238895034

Gene position: 2918791-2919384 (Clockwise)

Preceding gene: 238895033

Following gene: 238895036

Centisome position: 55.61

GC content: 58.42

Gene sequence:

>594_bases
ATGTCCGAGAACGCTCAACTGAATGGTCTGTGCGACCGTTTCCGCGGTTTTTATCCGGTGGTGATCGATGTTGAAACCGC
AGGATTTAACGCCAAAACCGACGCCCTGCTGGAGATCGCCGCCATTACGTTGAAGATGGATGAGCACGGCTGGCTGATGC
CCGATGAAACGCTGCATTTCCATGTTGAACCTTTCGAGGGCGCCAATCTGCAGCCCGAAGCGCTGGCTTTTAACGGGATT
AACCCGCATGACCCGCAGCGCGGGGCCGTCAGCGAATACGATGCCCTGCATGCCATCTTCAAAATGGTGCGCAAAGGGAT
GAAAGAGAGCGACTGCAGCCGGGCAATCATGGTGGCGCACAATGCCACCTTTGACCACAGCTTCACCATGACCGCCGCCG
AACGCGCGGGACTTAAACGCAACCCTTTCCATCCGTTTGTTACCTTCGACACCGCCGCGCTAAGCGGGCTGGCGCTGGGC
CAGACGGTGCTGTCGAAAGCCTGTATTGCCGCGGGTATGCCGTTCGACGGCGCGCAGCTCACTCCGCCCTGTATGATACC
GAGCAGACGGCGCAGCTGTTTTGCGAGATCGTGA

Upstream 100 bases:

>100_bases
CCCGGGCGCTACGGCGCCCGTTGTTTTCACCCCTGCCTGCTGCAAGCCGCGGCAAAATTTGCCATAATGCGCGCTACTTT
TTTCCAGCTAAGAGAATCTG

Downstream 100 bases:

>100_bases
ACCGCTGGAAGCGTCTGGGCGGCTGGCCGCTGCCAGTGGCCACCCCGGAGTGATAAAAAAAGCGACTTCCAGGAAGTCGC
TTTTTTACTGATGGCGGGAA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGI
NPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALG
QTVLSKACIAAGMPFDGAQLTPPCMIPSRRRSCFARS

Sequences:

>Translated_197_residues
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGI
NPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALG
QTVLSKACIAAGMPFDGAQLTPPCMIPSRRRSCFARS
>Mature_196_residues
SENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHFHVEPFEGANLQPEALAFNGIN
PHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAHNATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQ
TVLSKACIAAGMPFDGAQLTPPCMIPSRRRSCFARS

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=179, Percent_Identity=85.4748603351955, Blast_Score=320, Evalue=5e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 21586; Mature: 21455

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHF
CCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHEEEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGINPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAH
EECCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
NATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMPFDGAQL
CCCCCCEEEEHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPPCMIPSRRRSCFARS
CCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
SENAQLNGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEHGWLMPDETLHF
CCCCCCHHHHHHHCCCEEEEEEEEECCCCCCHHHHEEEEEEEEEECCCCCCCCCCCEEE
HVEPFEGANLQPEALAFNGINPHDPQRGAVSEYDALHAIFKMVRKGMKESDCSRAIMVAH
EECCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
NATFDHSFTMTAAERAGLKRNPFHPFVTFDTAALSGLALGQTVLSKACIAAGMPFDGAQL
CCCCCCEEEEHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TPPCMIPSRRRSCFARS
CCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA